{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:04:23Z","timestamp":1791821063467,"version":"4.3.4"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T00:00:00Z","timestamp":1745971200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Horphag Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Gingival inflammation is highly prevalent and may impact systemic health. While professional mechanical plaque removal (PMPR) is the standard treatment, dietary interventions may provide additional benefits. The French maritime pine bark extract Pycnogenol\u00ae has anti-inflammatory and antioxidant properties, but its impact on inflammatory biomarkers in saliva and serum has not been studied in a controlled clinical trial. Methods: In this randomized, double-blind, placebo-controlled clinical trial, 91 participants received Pycnogenol\u00ae (100 mg twice daily; n = 46) or a placebo (n = 45) following PMPR. Saliva and serum samples were collected at baseline, and after two and three months. Inflammatory biomarkers (IL-1\u03b2, IL-6, MMP-8, and MMP-9) and polyphenol concentrations were analyzed using ELISA and LC-MS\/MS. Results: Pycnogenol\u00ae supplementation significantly reduced salivary MMP-8 levels (p = 0.0261), and serum IL-6 levels compared to placebo (p = 0.0409). Additionally, ferulic acid, caffeic acid, and the gut microbial metabolite 5-(3,4-dihydroxyphenyl)-\u03b3-valerolactone (M1) significantly increased in saliva following Pycnogenol\u00ae intake. A correlation analysis revealed a significant inverse association between bleeding on probing and M1 concentration in saliva (r = \u22120.3476, p = 0.0167). Conclusions: Dietary supplementation with Pycnogenol\u00ae significantly reduced key inflammatory biomarkers and increased polyphenol concentrations in saliva, suggesting a potential anti-inflammatory effect of Pycnogenol\u00ae on gingival inflammation. Trial registration: ClinicalTrials.gov (NCT05786820).<\/jats:p>","DOI":"10.3390\/nu17091546","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T05:10:32Z","timestamp":1746076232000},"page":"1546","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Impact of a Dietary Supplementation with French Maritime Pine Bark Extract Pycnogenol\u00ae on Salivary and Serum Inflammatory Biomarkers During Non-Surgical Periodontal Therapy\u2014A Randomized Placebo-Controlled Double-Blind Trial"],"prefix":"10.3390","volume":"17","author":[{"given":"Jasmin","family":"Bayer","sequence":"first","affiliation":[{"name":"Institute of Pharmacy and Food Chemistry, University of W\u00fcrzburg, 97074 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole Karoline","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Periodontology, University Hospital W\u00fcrzburg, 97070 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanine Veruschka","family":"Hess","sequence":"additional","affiliation":[{"name":"Department of Periodontology, University Hospital W\u00fcrzburg, 97070 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5646-3691","authenticated-orcid":false,"given":"Yvonne","family":"Jockel-Schneider","sequence":"additional","affiliation":[{"name":"Department of Periodontology, University Hospital W\u00fcrzburg, 97070 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5752-8311","authenticated-orcid":false,"given":"Petra","family":"H\u00f6gger","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Food Chemistry, University of W\u00fcrzburg, 97074 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Arango Duque, G., and Descoteaux, A. 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