{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:26:59Z","timestamp":1767374819387,"version":"build-2065373602"},"reference-count":70,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"DALIM BIOTECH Corporation","award":["KHU-DALIM BIOTECH-20212131"],"award-info":[{"award-number":["KHU-DALIM BIOTECH-20212131"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background\/Objectives: Obesity is a major health concern that can lead to various chronic diseases. Little is known about the anti-obesity effect of a standardized hot water extract from the stems of Ipomoea batatas (WIB). This study aimed to evaluate the therapeutic potential of WIB as a natural alternative to conventional anti-obesity treatments by assessing its effects on body weight, fat accumulation, and key metabolic biomarkers in a high-fat diet-induced obesity model. Methods: A high-fat diet (HFD) induced obesity in C57BL\/6 mice. The mice were then treated orally with either orlistat (positive control) or WIB. Changes in body weight, food intake, and fat weight were measured, along with blood lipid profiles and adipokines. Western blot analyses were conducted to determine protein levels in each tissue. H&amp;E staining in white adipose tissue and liver, and the gut microbiota composition were analyzed. Results: WIB treatment significantly reduced body weight and fat mass compared to the HFD group and demonstrated comparable effects to orlistat. WIB improved blood lipid profiles and adipokine levels. H&amp;E staining revealed reduced fat accumulation in the white adipose tissue and liver. Also in those tissues, WIB restored expression levels of sterol regulatory element-binding protein-1 (SREBP-1) and CCAAT\/enhancer-binding protein \u03b1 (C\/EBP\u03b1) and increased AMP-activated protein kinase (AMPK) phosphorylation. In brown adipose tissue, WIB enhanced AMPK phosphorylation and upregulated thermogenic-related proteins, including peroxisome proliferator-activated receptor-gamma coactivator-1\u03b1 (PGC-1\u03b1), peroxisome proliferator-activated receptor \u03b1 (PPAR\u03b1), sirtuin 1 (SIRT1), uncoupling protein-1 (UCP-1), and cytochrome C oxidase subunit 4 (COX-IV). Analysis of gut microbiota revealed that WIB normalized \u03b2-diversity and reversed HFD-induced phyla imbalances (notably in Bacteroidetes, Firmicutes, and Proteobacteria). Conclusions: By reducing adiposity under the conditions tested in a murine model, improving metabolic markers, and favorably modulating gut microbiota, WIB demonstrates potential in mitigating obesity-related risks. These findings suggest that WIB may serve as a promising natural substance for the management of obesity. Further studies are warranted to confirm its efficacy and explore the potential underlying mechanisms in overweight or obese humans as a health supplement to help manage or prevent obesity.<\/jats:p>","DOI":"10.3390\/nu17101643","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T06:17:08Z","timestamp":1747030628000},"page":"1643","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Protective Effects of a Standardized Water Extract from the Stem of Ipomoea batatas L. Against High-Fat Diet-Induced Obesity"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8187-7734","authenticated-orcid":false,"given":"Chae-Won","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Fundamental Pharmaceutical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea"},{"name":"Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye Seul","family":"Yoon","sequence":"additional","affiliation":[{"name":"Development Department, Dalim Biotech Co., Ltd., Wonju-si 26348, Gangwon-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6386-5954","authenticated-orcid":false,"given":"Young-Seo","family":"Yoon","sequence":"additional","affiliation":[{"name":"BELABEL BIO. Inc., Gimhae-si 50969, Gyeongsangnam-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung-Sook","family":"Chung","sequence":"additional","affiliation":[{"name":"BELABEL BIO. Inc., Gimhae-si 50969, Gyeongsangnam-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi-ju","family":"Kim","sequence":"additional","affiliation":[{"name":"Development Department, Dalim Biotech Co., Ltd., Wonju-si 26348, Gangwon-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7072-2602","authenticated-orcid":false,"given":"Geonha","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea"},{"name":"Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minsik","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea"},{"name":"Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5865-9228","authenticated-orcid":false,"given":"Young-Pyo","family":"Jang","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea"},{"name":"Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea"},{"name":"Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea"},{"name":"Department of Integrated Drug Development and Natural Products, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3141-3727","authenticated-orcid":false,"given":"Kyung-Tae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Fundamental Pharmaceutical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea"},{"name":"Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S231","DOI":"10.1089\/dia.2024.2516","article-title":"Obesity, NAFLD\/NASH, and Diabetes","volume":"26","author":"Prattichizzo","year":"2024","journal-title":"Diabetes Technol. Ther."},{"doi-asserted-by":"crossref","unstructured":"Blackstone, R.P., and Blackstone, R.P. (2016). Obesity-related diseases and syndromes: Insulin resistance, type 2 diabetes mellitus, non-alcoholic fatty liver disease, cardiovascular disease, and metabolic syndrome. Obes. Med. Pract. Essent. Guide, 83\u2013108.","key":"ref_2","DOI":"10.1007\/978-3-319-39409-1_5"},{"doi-asserted-by":"crossref","unstructured":"Longo, M., Zatterale, F., Naderi, J., Parrillo, L., Formisano, P., Raciti, G.A., Beguinot, F., and Miele, C. (2019). Adipose Tissue Dysfunction as Determinant of Obesity-Associated Metabolic Complications. Int. J. Mol. Sci., 20.","key":"ref_3","DOI":"10.3390\/ijms20092358"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1080\/21623945.2020.1870060","article-title":"Brown and beige adipose tissue: A novel therapeutic strategy for obesity and type 2 diabetes mellitus","volume":"10","author":"Cheng","year":"2021","journal-title":"Adipocyte"},{"doi-asserted-by":"crossref","unstructured":"Petito, G., Cioffi, F., Magnacca, N., de Lange, P., Senese, R., and Lanni, A. (2023). Adipose Tissue Remodeling in Obesity: An Overview of the Actions of Thyroid Hormones and Their Derivatives. Pharmaceuticals, 16.","key":"ref_5","DOI":"10.3390\/ph16040572"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"C375","DOI":"10.1152\/ajpcell.00379.2020","article-title":"Adipose tissue inflammation and metabolic dysfunction in obesity","volume":"320","author":"Kawai","year":"2021","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2672","DOI":"10.1039\/D0FO02185G","article-title":"Standardized hot water extract from the leaves of Hydrangea serrata (Thunb.) Ser. alleviates obesity via the AMPK pathway and modulation of the gut microbiota composition in high fat diet-induced obese mice","volume":"12","author":"Han","year":"2021","journal-title":"Food Funct."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"6424","DOI":"10.1039\/D4FO00759J","article-title":"Anti-obesity effects of a standardized ethanol extract of Eisenia bicyclis by regulating the AMPK signaling pathway in 3T3-L1 cells and HFD-induced mice","volume":"15","author":"Yoon","year":"2024","journal-title":"Food Funct."},{"doi-asserted-by":"crossref","unstructured":"Abu Shelbayeh, O., Arroum, T., Morris, S., and Busch, K.B. (2023). PGC-1alpha Is a Master Regulator of Mitochondrial Lifecycle and ROS Stress Response. Antioxidants, 12.","key":"ref_9","DOI":"10.3390\/antiox12051075"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1172\/JCI10268","article-title":"Peroxisome proliferator-activated receptor gamma coactivator-1 promotes cardiac mitochondrial biogenesis","volume":"106","author":"Lehman","year":"2000","journal-title":"J. Clin. Investig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"176737","DOI":"10.1016\/j.ejphar.2024.176737","article-title":"Diosgenin attenuates nonalcoholic hepatic steatosis through the hepatic SIRT1\/PGC-1alpha pathway","volume":"977","author":"Meng","year":"2024","journal-title":"Eur. J. Pharmacol."},{"doi-asserted-by":"crossref","unstructured":"Cheng, C.F., Ku, H.C., and Lin, H. (2018). PGC-1alpha as a Pivotal Factor in Lipid and Metabolic Regulation. Int. J. Mol. Sci., 19.","key":"ref_12","DOI":"10.3390\/ijms19113447"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"S17","DOI":"10.1093\/advances\/nmy078","article-title":"Diet, Gut Microbiota, and Obesity: Links with Host Genetics and Epigenetics and Potential Applications","volume":"10","author":"Milagro","year":"2019","journal-title":"Adv. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s11894-022-00859-0","article-title":"Gut Microbiome and Its Impact on Obesity and Obesity-Related Disorders","volume":"25","author":"Sankararaman","year":"2023","journal-title":"Curr. Gastroenterol. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.freeradbiomed.2016.11.037","article-title":"Protective effect of quercetin on high-fat diet-induced non-alcoholic fatty liver disease in mice is mediated by modulating intestinal microbiota imbalance and related gut-liver axis activation","volume":"102","author":"Porras","year":"2017","journal-title":"Free Radic. Biol. Med."},{"doi-asserted-by":"crossref","unstructured":"Zs\u00e1lig, D., Berta, A., T\u00f3th, V., Szab\u00f3, Z., Simon, K., Figler, M., Pusztafalvi, H., and Poly\u00e1k, \u00c9. (2023). A review of the relationship between gut microbiome and obesity. Appl. Sci., 13.","key":"ref_16","DOI":"10.3390\/app13010610"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1126\/science.1243787","article-title":"Microbiology. Fighting obesity with bacteria","volume":"341","author":"Walker","year":"2013","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1186\/s13098-024-01561-z","article-title":"Understanding the complex function of gut microbiota: Its impact on the pathogenesis of obesity and beyond: A comprehensive review","volume":"16","author":"Yarahmadi","year":"2024","journal-title":"Diabetol. Metab. Syndr."},{"doi-asserted-by":"crossref","unstructured":"Kimura, I., Inoue, D., Hirano, K., and Tsujimoto, G. (2014). The SCFA Receptor GPR43 and Energy Metabolism. Front. Endocrinol., 5.","key":"ref_19","DOI":"10.3389\/fendo.2014.00085"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1007\/s13679-020-00422-w","article-title":"Long-Term Efficacy and Safety of Anti-Obesity Treatment: Where Do We Stand?","volume":"10","author":"Tak","year":"2021","journal-title":"Curr. Obes. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1517\/14740338.2015.994502","article-title":"Safety assessment of FDA-approved (orlistat and lorcaserin) anti-obesity medications","volume":"14","author":"Halpern","year":"2015","journal-title":"Expert Opin. Drug Saf."},{"doi-asserted-by":"crossref","unstructured":"Kim, G.N., Shin, M.R., Shin, S.H., Lee, A.R., Lee, J.Y., Seo, B.I., Kim, M.Y., Kim, T.H., Noh, J.S., and Rhee, M.H. (2016). Study of Antiobesity Effect Through Inhibition of Pancreatic Lipase Activity of Diospyros Kaki Fruit and Citrus Unshiu Peel. BioMed Res. Int., 2016.","key":"ref_22","DOI":"10.1155\/2016\/1723042"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"13","DOI":"10.4093\/dmj.2012.36.1.13","article-title":"Anti-Obesity Drugs: A Review About Their Effects and Safety","volume":"36","author":"Kang","year":"2012","journal-title":"Diabetes Metab. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1159\/000214834","article-title":"Green tea (-)-epigallocatechin-3-gallate reduces body weight with regulation of multiple genes expression in adipose tissue of diet-induced obese mice","volume":"54","author":"Lee","year":"2009","journal-title":"Ann. Nutr. Metab."},{"doi-asserted-by":"crossref","unstructured":"Barrios-Nolasco, A., Dominguez-Lopez, A., Miliar-Garcia, A., Cornejo-Garrido, J., and Jaramillo-Flores, M.E. (2023). Anti-Inflammatory Effect of Ethanolic Extract from Tabebuia rosea (Bertol.) DC., Quercetin, and Anti-Obesity Drugs in Adipose Tissue in Wistar Rats with Diet-Induced Obesity. Molecules, 28.","key":"ref_25","DOI":"10.3390\/molecules28093801"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.foodchem.2016.03.079","article-title":"Antioxidants and alpha-glucosidase inhibitors from Ipomoea batatas leaves identified by bioassay-guided approach and structure-activity relationships","volume":"208","author":"Zhang","year":"2016","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4117","DOI":"10.1039\/D0FO02733B","article-title":"Sweet potato (Ipomoea batatas L.) leaf polyphenols ameliorate hyperglycemia in type 2 diabetes mellitus mice","volume":"12","author":"Luo","year":"2021","journal-title":"Food Funct."},{"doi-asserted-by":"crossref","unstructured":"Shih, C.K., Chen, C.M., Varga, V., Shih, L.C., Chen, P.R., Lo, S.F., Shyur, L.F., and Li, S.C. (2020). White sweet potato ameliorates hyperglycemia and regenerates pancreatic islets in diabetic mice. Food Nutr. Res., 64.","key":"ref_28","DOI":"10.29219\/fnr.v64.3609"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"126302","DOI":"10.1155\/2015\/126302","article-title":"Purple Sweet Potato Leaf Extract Induces Apoptosis and Reduces Inflammatory Adipokine Expression in 3T3-L1 Differentiated Adipocytes","volume":"2015","author":"Lee","year":"2015","journal-title":"Evid. Based Complement. Altern. Med."},{"doi-asserted-by":"crossref","unstructured":"Lee, C.L., Lee, S.L., Chen, C.J., Chen, H.C., Kao, M.C., Liu, C.H., Chen, J.Y., Lai, Y.T., and Wu, Y.C. (2016). Characterization of Secondary Metabolites from Purple Ipomoea batatas Leaves and Their Effects on Glucose Uptake. Molecules, 21.","key":"ref_30","DOI":"10.3390\/molecules21060745"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.biochi.2023.04.008","article-title":"Adipokines in obesity and metabolic-related-diseases","volume":"212","author":"Pestel","year":"2023","journal-title":"Biochimie"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"102114","DOI":"10.1016\/j.gde.2023.102114","article-title":"Adipose tissue lipid metabolism: Lipolysis","volume":"83","author":"Cho","year":"2023","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1007\/s00109-021-02176-x","article-title":"Recapitulating lipid accumulation and related metabolic dysregulation in human liver-derived organoids","volume":"100","author":"Wang","year":"2022","journal-title":"J. Mol. Med."},{"doi-asserted-by":"crossref","unstructured":"Saito, M., Matsushita, M., Yoneshiro, T., and Okamatsu-Ogura, Y. (2020). Brown Adipose Tissue, Diet-Induced Thermogenesis, and Thermogenic Food Ingredients: From Mice to Men. Front. Endocrinol., 11.","key":"ref_34","DOI":"10.3389\/fendo.2020.00222"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1017\/S0007114520000380","article-title":"Microbial dysbiosis-induced obesity: Role of gut microbiota in homoeostasis of energy metabolism","volume":"123","author":"Amabebe","year":"2020","journal-title":"Br. J. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12263-021-00703-6","article-title":"Gut microbiota in patients with obesity and metabolic disorders\u2014A systematic review","volume":"17","author":"Xu","year":"2022","journal-title":"Genes. Nutr."},{"doi-asserted-by":"crossref","unstructured":"(2021). Obesity: Causes, consequences, treatments, and challenges. J. Mol. Cell Biol., 13, 463\u2013465.","key":"ref_37","DOI":"10.1093\/jmcb\/mjab056"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s13237-023-00420-y","article-title":"Contribution of environmental, genetic and epigenetic factors to obesity-related metabolic syndrome","volume":"66","author":"Ghosh","year":"2023","journal-title":"Nucleus"},{"doi-asserted-by":"crossref","unstructured":"Smits, M.M., and Van Raalte, D.H. (2021). Safety of Semaglutide. Front. Endocrinol., 12.","key":"ref_39","DOI":"10.3389\/fendo.2021.645563"},{"doi-asserted-by":"crossref","unstructured":"Shaik Mohamed Sayed, U.F., Moshawih, S., Goh, H.P., Kifli, N., Gupta, G., Singh, S.K., Chellappan, D.K., Dua, K., Hermansyah, A., and Ser, H.L. (2023). Natural products as novel anti-obesity agents: Insights into mechanisms of action and potential for therapeutic management. Front. Pharmacol., 14.","key":"ref_40","DOI":"10.3389\/fphar.2023.1182937"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ijbiomac.2022.12.002","article-title":"Resistant starch from sweet potatoes: Recent advancements and applications in the food sector","volume":"225","author":"Bodjrenou","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.foodchem.2009.05.005","article-title":"Effect of photoperiod on flavonoid pathway activity in sweet potato (Ipomoea batatas (L.) Lam.) leaves","volume":"118","author":"Carvalho","year":"2010","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"165","DOI":"10.4103\/0973-1296.80682","article-title":"Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves\u2019 extract from Ipomoea batatas (L.) Lam","volume":"7","author":"Pochapski","year":"2011","journal-title":"Pharmacogn. Mag."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"9966750","DOI":"10.1155\/2022\/9966750","article-title":"Flavonols and Flavones as Potential anti-Inflammatory, Antioxidant, and Antibacterial Compounds","volume":"2022","author":"Chagas","year":"2022","journal-title":"Oxid. Med. Cell. Longev."},{"doi-asserted-by":"crossref","unstructured":"Farhan, M. (2022). Green Tea Catechins: Nature\u2019s Way of Preventing and Treating Cancer. Int. J. Mol. Sci., 23.","key":"ref_45","DOI":"10.3390\/ijms231810713"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"421","DOI":"10.2147\/JIR.S228361","article-title":"Modulation of Chronic Inflammation by Quercetin: The Beneficial Effects on Obesity","volume":"13","author":"Sato","year":"2020","journal-title":"J. Inflamm. Res."},{"doi-asserted-by":"crossref","unstructured":"White, U. (2023). Adipose tissue expansion in obesity, health, and disease. Front. Cell Dev. Biol., 11.","key":"ref_47","DOI":"10.3389\/fcell.2023.1188844"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s13679-023-00518-z","article-title":"Obesity and Dyslipidemia: A Review of Current Evidence","volume":"12","author":"Vekic","year":"2023","journal-title":"Curr. Obes. Rep."},{"doi-asserted-by":"crossref","unstructured":"Huang, J.K., and Lee, H.C. (2022). Emerging Evidence of Pathological Roles of Very-Low-Density Lipoprotein (VLDL). Int. J. Mol. Sci., 23.","key":"ref_49","DOI":"10.3390\/ijms23084300"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"E653","DOI":"10.1152\/ajpendo.00620.2020","article-title":"100(th) anniversary of the discovery of insulin perspective: Insulin and adipose tissue fatty acid metabolism","volume":"320","author":"Carpentier","year":"2021","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/s41467-020-20500-w","article-title":"High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance","volume":"12","author":"Kumar","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.cca.2012.12.007","article-title":"Role of leptin and adiponectin in insulin resistance","volume":"417","author":"Yadav","year":"2013","journal-title":"Clin. Chim. Acta"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1007\/BF03344048","article-title":"Adipose tissue hormones","volume":"25","year":"2002","journal-title":"J. Endocrinol. Investig."},{"key":"ref_54","first-page":"23","article-title":"Regulation of Carbohydrate Metabolism, Lipid Metabolism, and Protein Metabolism by AMPK","volume":"107","author":"Angin","year":"2016","journal-title":"Exp. Suppl."},{"doi-asserted-by":"crossref","unstructured":"Zhang, S., Yan, L., and Kim, S.M. (2020). Vanadium-protein complex inhibits human adipocyte differentiation through the activation of beta-catenin and LKB1\/AMPK signaling pathway. PLoS ONE, 15.","key":"ref_55","DOI":"10.1371\/journal.pone.0239547"},{"doi-asserted-by":"crossref","unstructured":"Ambele, M.A., Dhanraj, P., Giles, R., and Pepper, M.S. (2020). Adipogenesis: A Complex Interplay of Multiple Molecular Determinants and Pathways. Int. J. Mol. Sci., 21.","key":"ref_56","DOI":"10.3390\/ijms21124283"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1007\/s11033-020-06036-8","article-title":"Molecular mechanism of down-regulating adipogenic transcription factors in 3T3-L1 adipocyte cells by bioactive anti-adipogenic compounds","volume":"48","author":"Guru","year":"2021","journal-title":"Mol. Biol. Rep."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1038\/s41556-023-01311-9","article-title":"EPAC1 enhances brown fat growth and beige adipogenesis","volume":"26","author":"Siddig","year":"2024","journal-title":"Nat. Cell Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"E488","DOI":"10.1152\/ajpendo.00310.2020","article-title":"An update on brown adipose tissue biology: A discussion of recent findings","volume":"320","author":"Gaspar","year":"2021","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s12986-022-00694-0","article-title":"Browning of the white adipose tissue regulation: New insights into nutritional and metabolic relevance in health and diseases","volume":"19","author":"Machado","year":"2022","journal-title":"Nutr. Metab."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1210\/endrev\/bnac015","article-title":"Brown Adipose Tissue\u2014A Translational Perspective","volume":"44","author":"Carpentier","year":"2023","journal-title":"Endocr. Rev."},{"doi-asserted-by":"crossref","unstructured":"Wu, L., Zhang, L., Li, B., Jiang, H., Duan, Y., Xie, Z., Shuai, L., Li, J., and Li, J. (2018). AMP-Activated Protein Kinase (AMPK) Regulates Energy Metabolism Through Modulating Thermogenesis in Adipose Tissue. Front. Physiol., 9.","key":"ref_62","DOI":"10.3389\/fphys.2018.00122"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1017\/S0029665114001700","article-title":"Gut microbiota and energy balance: Role in obesity","volume":"74","author":"Blaut","year":"2015","journal-title":"Proc. Nutr. Soc."},{"doi-asserted-by":"crossref","unstructured":"Wu, X., Zhang, G., Zhang, W., Zhou, J., Cong, H., Yang, G., and Liu, G. (2024). Rumen microbiota helps Tibetan sheep obtain energy more efficiently to survive in the extreme environment of the Qinghai-Tibet Plateau. Front. Microbiol., 15.","key":"ref_64","DOI":"10.3389\/fmicb.2024.1431063"},{"doi-asserted-by":"crossref","unstructured":"Stojanov, S., Berlec, A., and Strukelj, B. (2020). The Influence of Probiotics on the Firmicutes\/Bacteroidetes Ratio in the Treatment of Obesity and Inflammatory Bowel Disease. Microorganisms, 8.","key":"ref_65","DOI":"10.3390\/microorganisms8111715"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.2174\/1389450123666220429082702","article-title":"Targeting AMPK Signaling in the Liver: Implications for Obesity and Type 2 Diabetes Mellitus","volume":"23","author":"Wang","year":"2022","journal-title":"Curr. Drug Targets"},{"doi-asserted-by":"crossref","unstructured":"Lee, S.Y., Chung, K.S., Son, S.R., Lee, S.Y., Jang, D.S., Lee, J.K., Kim, H.J., Na, C.S., Lee, S.H., and Lee, K.T. (2022). A Botanical Mixture Consisting of Inula japonica and Potentilla chinensis Relieves Obesity via the AMPK Signaling Pathway in 3T3-L1 Adipocytes and HFD-Fed Obese Mice. Nutrients, 14.","key":"ref_67","DOI":"10.3390\/nu14183685"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"3837","DOI":"10.3748\/wjg.v27.i25.3837","article-title":"Gut microbiota in obesity","volume":"27","author":"Liu","year":"2021","journal-title":"World J. Gastroenterol."},{"key":"ref_69","first-page":"308","article-title":"The human gut microbiota: Metabolism and perspective in obesity","volume":"9","author":"Gomes","year":"2018","journal-title":"Gut Microbes"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1038\/s41467-022-28743-5","article-title":"Intestinal AMPK modulation of microbiota mediates crosstalk with brown fat to control thermogenesis","volume":"13","author":"Zhang","year":"2022","journal-title":"Nat. Commun."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/10\/1643\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:31:06Z","timestamp":1760031066000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/10\/1643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,12]]},"references-count":70,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["nu17101643"],"URL":"https:\/\/doi.org\/10.3390\/nu17101643","relation":{},"ISSN":["2072-6643"],"issn-type":[{"type":"electronic","value":"2072-6643"}],"subject":[],"published":{"date-parts":[[2025,5,12]]}}}