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Recent studies have demonstrated that probiotics may alleviate constipation by restoring gut microbiota balance. Methods: This study investigated the effects of Weizmannia coagulans BC99 (formerly Bacillus coagulans BC99) on gut microbiota and intestinal function in a loperamide-induced mouse model of functional constipation. BALB\/c mice were randomly divided into six groups: control, model, phenolphthalein, BC99-L (2 \u00d7 107 CFU\/day), BC99-M (2 \u00d7 108 CFU\/day), and BC99-H (2 \u00d7 109 CFU\/day). After 14 days of supplementation, constipation was induced in mice via loperamide administration. Results: BC99 significantly increased fecal water content, gastrointestinal transit rate, microbial metabolic activity, and butyric acid production, while decreasing the abundance of inflammation-related metabolic pathways. Moreover, BC99 improved levels of gastrointestinal regulatory peptides, including motilin and somatostatin. The Firmicutes-to-Bacteroidetes ratio was elevated in the BC99-M and the BC99-H group compared to the model group, indicating that BC99 effectively modulated gut microbiota composition and host biosynthetic pathways. Conclusions:\u00a0 Weizmannia coagulans BC99 alleviated and prevented loperamide-induced functional constipation in mice by enhancing intestinal peristalsis and restoring gut microbial homeostasis.<\/jats:p>","DOI":"10.3390\/nu17101729","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T06:54:28Z","timestamp":1747724068000},"page":"1729","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Weizmannia Coagulans BC99 Prevents Loperamide-Induced Functional Constipation in Mice Through Increased Intestinal Peristalsis and Modulation of Gut Microbiota Dysbiosis"],"prefix":"10.3390","volume":"17","author":[{"given":"Cheng","family":"Li","sequence":"first","affiliation":[{"name":"College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China"},{"name":"Henan Engineering Research Center of Food Microbiology, Luoyang 450001, China"},{"name":"National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China"},{"name":"Department of Research and Development, Wecare Probiotics Co., Ltd., Suzhou 215200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Wecare Probiotics Co., Ltd., Suzhou 215200, China"},{"name":"Germline Stem Cells and Microenvironment Lab, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuguang","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Wecare Probiotics Co., Ltd., Suzhou 215200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan-Young","family":"Jeong","sequence":"additional","affiliation":[{"name":"Life Science Research Institute, Novarex Co., Ltd., Cheongju 28220, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye-Rim","family":"Kim","sequence":"additional","affiliation":[{"name":"Life Science Research Institute, Novarex Co., Ltd., Cheongju 28220, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2283-0318","authenticated-orcid":false,"given":"Shaobin","family":"Gu","sequence":"additional","affiliation":[{"name":"College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China"},{"name":"Henan Engineering Research Center of Food Microbiology, Luoyang 450001, China"},{"name":"National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/s13765-016-0182-7","article-title":"Effect of microencapsulated Bacillus subtilis strain CBD2-fermented grain on loperamide-induced constipation in mice","volume":"59","author":"Kim","year":"2016","journal-title":"Appl. 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