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Despite its popularity, there is a notable lack of scientific research addressing the metabolic and immune responses of elite female athletes in this sport. This study aims to address this gap by investigating two world-class Olympic medalists, female BVb players, who represent a country with a rich history in the sport. Methods: Two athletes underwent a simulated competition day consisting of two matches. A standardized protocol was utilized to collect blood and urine samples at seven time points, allowing for analysis throughout the competition and recovery phases. The analysis included various electrolytes, as well as hematological, metabolic, and inflammatory markers. Additionally, we assessed selected hormones, such as insulin, serotonin, ACTH, and cortisol, along with amino acids related to energy metabolism and neurotransmitter synthesis. Results: Both athletes presented a trend toward electrolyte disturbances, especially hypokalemia, with a mean decrease of 15% and individual values reaching as low as 3.3 mmol\/L post-match. This indicates that BVb may pose a risk for such disturbances. Additionally, the matches led to 20% to 60% increases in muscle injury markers, with incomplete recovery even after a day of rest, signaling persistent physiological stress post-competition. This increase was matched by stimulating stress hormones (ACTH and cortisol rose up to 4-fold and 3-fold, respectively), and markers of exercise intensity, such as lactate and ammonium. Moreover, the simulated BVb competition day impacted the amino acid response, with the Fischer ratio (BCAA\/AAA) and blood tryptophan decreasing to a minimum of 60% of the initial levels and blood serotonin increasing by up to 180%, which are signs of an increased risk of central fatigue onset, according to the Fischer and Newsholme theory. Conclusions: The responses examined in this exploratory study contribute to a deeper understanding of the metabolic and immune demands placed on elite female BVb players, suggesting practical applications. By addressing the similar physiological responses observed among the athletes and emphasizing their unique individual responses\u2014despite following the same protocol under identical conditions and sharing similar life habits for an extended period\u2014this study highlights the critical necessity for the n-of-1 monitoring of athletes.<\/jats:p>","DOI":"10.3390\/nu17111924","type":"journal-article","created":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T03:57:34Z","timestamp":1749009454000},"page":"1924","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Influence of Competition Day Loads on the Metabolic and Immune Response of Olympic Female Beach Volleyball Athletes: A Sportomics Analysis"],"prefix":"10.3390","volume":"17","author":[{"given":"Renan","family":"Muniz-Santos","sequence":"first","affiliation":[{"name":"Lorraine Protein Biochemistry Group, Graduate Program in Neurology, Gaffr\u00e9e e Guinle University Hospital, Rio de Janeiro 20270-004, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7817-0884","authenticated-orcid":false,"given":"Flavio","family":"Bachini","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Biochemistry, The Federal University of the State of Rio de Janeiro, Rio de Janeiro 20290-250, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Bassini","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Biochemistry, The Federal University of the State of Rio de Janeiro, Rio de Janeiro 20290-250, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Alexandre","sequence":"additional","affiliation":[{"name":"Lorraine Protein Biochemistry Group, Graduate Program in Neurology, Gaffr\u00e9e e Guinle University Hospital, Rio de Janeiro 20270-004, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Jurisica","sequence":"additional","affiliation":[{"name":"Osteoarthritis Research Program, Division of Orthopedic Surgery, Schroeder Arthritis Institute and Data Science Discovery Centre for Chronic Diseases, Krembil Research Institute, University Health Network, Toronto, ON M5T 0S8, Canada"},{"name":"Departments of Medical Biophysics and Computer Science, Faculty of Dentistry, University of Toronto, Toronto, ON M5G IL7, Canada"},{"name":"Institute of Neuroimmunology, Slovak Academy of Sciences, 845 10 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinod","family":"Chandran","sequence":"additional","affiliation":[{"name":"Gladman-Krembil Psoriatic Arthritis Program, Schroeder Arthritis Institute, Krembil Research Institute, University Health Network, Toronto, ON M5T 0S8, Canada"},{"name":"Division of Rheumatology, Department of Medicine, Institute of Medical Science, Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, ON M5T 0S8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4180-2061","authenticated-orcid":false,"given":"L.","family":"Cameron","sequence":"additional","affiliation":[{"name":"Lorraine Protein Biochemistry Group, Graduate Program in Neurology, Gaffr\u00e9e e Guinle University Hospital, Rio de Janeiro 20270-004, RJ, Brazil"},{"name":"Gladman-Krembil Psoriatic Arthritis Program, Schroeder Arthritis Institute, Krembil Research Institute, University Health Network, Toronto, ON M5T 0S8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e16736","DOI":"10.7717\/peerj.16736","article-title":"Differences in external load among indoor and beach volleyball players during elite matches","volume":"12","author":"Hank","year":"2024","journal-title":"PeerJ"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1113\/EP090969","article-title":"Delineating the impacts of air temperature and humidity for endurance exercise","volume":"108","author":"Jenkins","year":"2023","journal-title":"Exp. 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