{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:21:06Z","timestamp":1791400866760,"version":"4.3.3"},"reference-count":51,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Inne Nutrition and Health Research Fund for the Inne Institude of Nutrition and Health","award":["INHRF-GD-202407-031"],"award-info":[{"award-number":["INHRF-GD-202407-031"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Objective: Growth retardation in adolescents caused by nutritional deficiency requires effective intervention. A novel dietary supplement containing bamboo shoot extract, amino acids, and calcium citrate (Kidtal + Ca, KDTCa) was evaluated for its growth-promoting effects. Methods: After acclimatization, sixty-three 3-week-old male Sprague-Dawley (SD) rats were randomly divided into a normal control group and model groups. Growth retardation was induced in the modeling groups through calcium-deficient feeding, followed by administration of KDTCa, bamboo shoot extract and amino acids (Kidtal), or calcium citrate (CC). After 6 weeks of intragastric administration, the mechanical properties, microstructure, and growth plate development of bone were evaluated using three-point bending, micro-CT, and H&amp;E staining, respectively. Bone calcium\/phosphorus distribution and fecal calcium apparent absorption rate were measured by ICP-MS. Results: All inter-group differences were analyzed using one-way analysis of variance and checked using the Tuckey test. KDTCa treatment dose-dependently enhanced bone development in calcium-deficient rats. Compared to the model group, H-KDTCa significantly restored naso-anal length (p &lt; 0.05) and body weight (p &lt; 0.01). KDTCa supplementation significantly restored calcium and phosphorus levels in blood and bone. Three-point bending experiments showed that the stiffness and bending energy were increased by 142.58% and 384.7%. In bone microarchitecture, both bone mineral density (BMD) and microstructural parameters were significantly improved. These findings were consistent with the increased long bone length (p &lt; 0.05) and decreased serum BALP\/TRACP levels (p &lt; 0.001). Dose-dependent IGF-1 elevation (p &lt; 0.01) potentially mediated growth plate elongation by 35.34%. Notably, KDTCa increased calcium apparent absorption by 6.1% versus calcium-only supplementation at equal intake. Conclusions: KDTCa improves bone microstructure and strength, restores bone metabolism, and enhances growth plate height via promoting IGF-1 secretion to facilitate bone development. Further studies are needed to determine whether the components and calcium in Kidtal have a synergistic effect.<\/jats:p>","DOI":"10.3390\/nu17121966","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T05:11:49Z","timestamp":1749532309000},"page":"1966","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Calcium Supplement Combined with Dietary Supplement Kidtal Can Promote Longitudinal Growth of Long Bone in Calcium-Deficient Adolescent Rats"],"prefix":"10.3390","volume":"17","author":[{"given":"Haosheng","family":"Xie","sequence":"first","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Inne Institude of Nutrition and Health, Bismarckstra\u00dfe 37, 66121 Saarbr\u00fccken, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyang","family":"Guan","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunmei","family":"Shan","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwei","family":"Mi","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinfa","family":"Ye","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihui","family":"Liu","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yin","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Han","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1210\/edrv-8-4-426","article-title":"Mechanism of the Stimulatory Effect of Growth Hormone on Longitudinal Bone Growth","volume":"8","author":"Isaksson","year":"1987","journal-title":"Endocr. 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