{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T03:50:08Z","timestamp":1784001008239,"version":"3.55.0"},"reference-count":32,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background\/Objectives: Coeliac disease is an immune-mediated disorder of the gastrointestinal tract that may result in significant nutritional deficiencies. Effective management requires strict, lifelong adherence to a gluten-free diet. Both underdiagnosis and unnecessary dietary restrictions can adversely affect patients\u2019 health and quality of life. To assess adherence to the current recommendations for the laboratory diagnosis of coeliac disease and promote evidence-based practices while reducing inter-laboratory variability, the Spanish Group on Autoimmunity of the Spanish Society of Immunology conducted a nationwide survey. Methods: A thirty-item survey was distributed to fifty autoimmune laboratories across Spain. Data were collected through a structured Excel-based questionnaire comprising multiple-choice items, which was distributed via email to the participating laboratories. It explored practices related to the diagnosis of coeliac disease in the general population and among at-risk groups as well as approaches to patient follow-up and demand management. Results: Thirty-five laboratories completed the electronic questionnaire. For the serological screening of coeliac disease, all the respondents reported using IgA anti-tissue transglutaminase (tTG-IgA) antibody testing together with total IgA measurement to assess IgA competence. However, consistent use of anti-endomysial antibody testing and HLA genotyping and adherence to pre-analytical recommendations for accurate interpretation of results were not uniform across centres. Conclusions: At the time these data were collected (the third trimester of 2021), the 2020 ESPGHAN guidelines for the diagnosis of coeliac disease in the paediatric population had not yet been fully implemented in most of the laboratories surveyed. For diagnosing adults, most laboratories adhered to local and European guidelines.<\/jats:p>","DOI":"10.3390\/nu17122032","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T09:36:00Z","timestamp":1750239360000},"page":"2032","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Laboratory and Clinical Practices in the Study of Coeliac Disease in Children and Adults: Recommendations from a Spanish Multicentre Survey"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7120-5631","authenticated-orcid":false,"given":"Roc\u00edo","family":"Aguado","sequence":"first","affiliation":[{"name":"UGC Inmunologia-Alergologia, Maimonides Biomedical Research Institute of Cordoba (IMIBIC), Reina Sofia University Hospital of Cordoba, 14004 Cordoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2315-8671","authenticated-orcid":false,"given":"Juan","family":"Irure-Ventura","sequence":"additional","affiliation":[{"name":"Immunology Department, University Hospital Marqu\u00e9s de Valdecilla, 39008 Santander, Spain"},{"name":"Immunopathology Group, University Hospital Marqu\u00e9s de Valdecilla-IDIVAL, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4744-9986","authenticated-orcid":false,"given":"Maria Luisa","family":"Vargas","sequence":"additional","affiliation":[{"name":"Immunology and Genetics Department, Hospital Universitario de Badajoz, 06080 Badajoz, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garbi\u00f1e","family":"Roy","sequence":"additional","affiliation":[{"name":"Immunology Department, Hospital Universitario Ramon y Cajal, Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS), 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6994-2034","authenticated-orcid":false,"given":"Yvelise","family":"Barrios","sequence":"additional","affiliation":[{"name":"Immunology Laboratory, Hospital Universitario de Canarias, 38320 Santa Cruz de Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0126-158X","authenticated-orcid":false,"given":"Laura","family":"Mart\u00ednez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Immunology Department, Hospital de La Santa Creu I Sant Pau, Universitat Autonoma de Barcelona, C\/Sant Antoni M Claret 167, 08025 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0946-6002","authenticated-orcid":false,"given":"Beatriz","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Immunology Department, Hospital Universitario Virgen del Rocio (IBiS, CSIC, US), 41013 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Antonio","family":"Montes-Cano","sequence":"additional","affiliation":[{"name":"Immunology Department, Hospital Universitario Virgen del Rocio (IBiS, CSIC, US), 41013 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0562-427X","authenticated-orcid":false,"given":"Marcos","family":"L\u00f3pez-Hoyos","sequence":"additional","affiliation":[{"name":"Immunology Department, University Hospital Marqu\u00e9s de Valdecilla, 39008 Santander, Spain"},{"name":"Immunopathology Group, University Hospital Marqu\u00e9s de Valdecilla-IDIVAL, 39011 Santander, Spain"},{"name":"Department of Molecular Biology, University of Cantabria, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9092-8758","authenticated-orcid":false,"given":"Aurora","family":"Jurado","sequence":"additional","affiliation":[{"name":"UGC Inmunologia-Alergologia, Maimonides Biomedical Research Institute of Cordoba (IMIBIC), Reina Sofia University Hospital of Cordoba, 14004 Cordoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Stanciu, D., Staykov, H., Dragomanova, S., Tancheva, L., Pop, R.S., Ielciu, I., and Crisan, G. 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