{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:31:14Z","timestamp":1760059874201,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2019-67017-29366"],"award-info":[{"award-number":["2019-67017-29366"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background\/Objectives: Colitis is a chronic condition affecting millions worldwide. Purple muscadine wine polyphenols have a unique composition and possible disease-preventive properties. This study aims to determine how dealcoholized muscadine wine (DMW) affects the development of colitis and gut microbiome in IL-10\u2212\/\u2212 mice, compared to wild types (WT). Methods: Six-week-old male IL-10\u2212\/\u2212 and WT C57BL\/6 mice were fed either a DMW-supplemented diet (4.8% v\/w) or a control diet based on AIN-93M for 154 days. Colitis severity was evaluated by disease activity, intestinal permeability, gene expression of cytokines and tight junction proteins in the colon, and inflammatory cytokines in the serum. Fecal samples were collected for gut microbiome profiling via 16S rRNA gene sequencing. Results: DMW contained predominantly anthocyanins and a significant amount of ellagic acid. IL-10\u2212\/\u2212 mice developed mild colitis as indicated by the disease activity index. DMW \u00d7 gene interactions decreased intestinal permeability, colonic mRNA levels of IL-1\u03b2, and serum TNF-\u03b1 in the IL-10\u2212\/\u2212 mice. DMW suppressed the colonic mRNA levels of IL-6, enhanced the gene expression of ZO-1, but did not influence the mRNA level of TNF-\u03b1 or occludin. While DMW did not alter \u03b1-diversity of the gut microbiome, it significantly influenced \u03b2-diversity in the WT mice. DMW significantly reduced the relative abundances of Akkermansia in the IL-10\u2212\/\u2212 and WT mice. DMW and DMW\u00d7gene interaction decreased the relative abundance of Parasutterella only in IL-10\u2212\/\u2212 mice. Conclusions: These results suggested that polyphenols from DMW interacted with genes to moderately alleviate the development of colitis in IL-10\u2212\/\u2212 mice and could be a useful dietary strategy for IBD prevention.<\/jats:p>","DOI":"10.3390\/nu17142327","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T08:24:17Z","timestamp":1752654257000},"page":"2327","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Dealcoholized Muscadine Wine on the Development of Spontaneous Colitis and Gut Microbiome in IL-10\u2212\/\u2212 Mice"],"prefix":"10.3390","volume":"17","author":[{"given":"Hao","family":"Li","sequence":"first","affiliation":[{"name":"Food Science and Human Nutrition Department, Institute of Food and Agriculture Sciences, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwei","family":"Gu","sequence":"additional","affiliation":[{"name":"Food Science and Human Nutrition Department, Institute of Food and Agriculture Sciences, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1038\/s41586-025-08940-0","article-title":"Global IBD Visualization of Epidemiology Studies in the 21st Century (GIVES-21) Research Group Global evolution of inflammatory bowel disease across epidemiologic stages","volume":"642","author":"Hracs","year":"2025","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Fanizza, J., Bencardino, S., Allocca, M., Furfaro, F., Zilli, A., Parigi, T.L., Fiorino, G., Peyrin-Biroulet, L., Danese, S., and D\u2019Amico, F. 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