{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T20:49:28Z","timestamp":1790455768084,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Asahi Soft Drinks Company","award":["20210402019GH"],"award-info":[{"award-number":["20210402019GH"]}]},{"name":"department of science and technology of Jilin province","award":["20210402019GH"],"award-info":[{"award-number":["20210402019GH"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: The differential effects of post-exercise rehydration beverages on inflammatory processes and organ protection remain incompletely characterized. This study investigated how beverages with distinct compositions influence urinary biomarkers following endurance exercise. Methods: In a randomized crossover design, eight trained male runners performed 6000 m pace running followed by consumption of 500 mL of either: water (Drink 1), hypotonic sports drink (Drink 2, 200 mOsm\/L), oral rehydration solution (Drink 3, 270 mOsm\/L), or modified hypotonic formulation (Drink 4, 200 mOsm\/L). After 60 min, participants completed a 1000 m time trial. Urine samples were collected at baseline, post-6000 m, and post-1000 m for analysis of biochemical parameters and inflammatory cytokines. Results: No significant differences in 1000 m performance were observed between trials. Drink 3 significantly reduced creatinine and uric acid excretion compared to other beverages (p &lt; 0.05), suggesting decreased waste product elimination. Creatinine-corrected intestinal fatty acid-binding protein values were lower with Drinks 2 and 3, indicating potential intestinal protection. Notably, Drink 4 showed modest but significant enhancement of IL-4 excretion (p &lt; 0.05, \u03b7p2 = 0.347), demonstrating beverage-specific modulation of anti-inflammatory cytokines with moderate effect sizes. Conclusions: Different beverage formulations exert distinct effects on waste product elimination, intestinal organ damage markers, and inflammatory cytokine profiles. These findings suggest that beverage selection should be tailored to specific recovery priorities and training contexts.<\/jats:p>","DOI":"10.3390\/nu17142379","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T07:56:14Z","timestamp":1753084574000},"page":"2379","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Beverage-Specific Modulation of Urinary Inflammatory Biomarkers After Endurance Running in Trained Males"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6572-5809","authenticated-orcid":false,"given":"Katsuhiko","family":"Suzuki","sequence":"first","affiliation":[{"name":"Faculty of Sport Sciences, Waseda University, Tokorozawa 359-1192, Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazue","family":"Kanda","sequence":"additional","affiliation":[{"name":"Faculty of Sport Sciences, Waseda University, Tokorozawa 359-1192, Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0606-5759","authenticated-orcid":false,"given":"Sihui","family":"Ma","sequence":"additional","affiliation":[{"name":"Faculty of Sport Sciences, Waseda University, Tokorozawa 359-1192, Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S102","DOI":"10.1519\/JSC.0000000000003298","article-title":"Effects of Hypohydration on Muscular Strength, Endurance, and Power in Women","volume":"35","author":"Gann","year":"2021","journal-title":"J. Strength Cond. Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ruggiero, M., Motti, M.L., Meccariello, R., and Mazzeo, F. (2025). Resveratrol and Physical Activity: A Successful Combination for the Maintenance of Health and Wellbeing?. Nutrients, 17.","DOI":"10.3390\/nu17050837"},{"key":"ref_3","first-page":"003","article-title":"Beyond the Hype: A Scientific Assessment of Sports Supplements","volume":"45","author":"Ganu","year":"2024","journal-title":"Int. J. Clin. Stud. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"55","DOI":"10.4085\/1062-6050-0682.22","article-title":"Oral Rehydration Beverages for Treating Exercise-Associated Dehydration: A Systematic Review, Part II. The Effectiveness of Alternatives to Carbohydrate-Electrolyte Drinks","volume":"60","author":"Borra","year":"2025","journal-title":"J. Athl. Train."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"34","DOI":"10.4085\/1062-6050-0682.22","article-title":"Oral rehydration beverages for treating exercise-associated dehydration: A systematic review, Part I. Carbohydrate-electrolyte solutions","volume":"60","author":"Borra","year":"2025","journal-title":"J. Athl. Train."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"10","DOI":"10.23937\/2469-5718\/1510122","article-title":"Characterization of exercise-induced cytokine release, the impacts on the body, the mechanisms and modulations","volume":"5","author":"Suzuki","year":"2019","journal-title":"Int. J. Sports Exerc. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1007\/s00421-018-3839-6","article-title":"Fluid intake restores retinal blood flow early after exhaustive exercise in healthy subjects","volume":"118","author":"Ikemura","year":"2018","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"H1598","DOI":"10.1152\/ajpheart.00525.2015","article-title":"Dehydration accelerates reductions in cerebral blood flow during prolonged exercise in the heat without compromising brain metabolism","volume":"309","author":"Trangmar","year":"2015","journal-title":"Am. J. Physiol.-Heart Circ. Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Tominaga, T., Ma, S., Sugama, K., Kanda, K., Omae, C., Choi, W., Hashimoto, S., Aoyama, K., Yoshikai, Y., and Suzuki, K. (2021). Changes in urinary biomarkers of organ damage, inflammation, oxidative stress, and bone turnover following a 3000-m time trial. Antioxidants, 10.","DOI":"10.3390\/antiox10010079"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2632","DOI":"10.1001\/jama.291.21.2632","article-title":"Clinical concerns about reduced-osmolarity oral rehydration solution","volume":"291","author":"Nalin","year":"2004","journal-title":"Jama"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kung, S., Vakula, M.N., Kim, Y., England, D.L., Bergeson, J., Bressel, E., Lefevre, M., and Ward, R. (2022). No effect of a dairy-based, high flavonoid pre-workout beverage on exercise-induced intestinal injury, permeability, and inflammation in recreational cyclists: A randomized controlled crossover trial. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0277453"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1123\/ijsnem.2018-0288","article-title":"Exercise-induced illness and inflammation: Can immunonutrition and iron help?","volume":"29","author":"Castell","year":"2019","journal-title":"Int. J. Sport Nutr. Exerc. Metab."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Belval, L.N., Hosokawa, Y., Casa, D.J., Adams, W.M., Armstrong, L.E., Baker, L.B., Burke, L., Cheuvront, S., Chiampas, G., and Gonz\u00e1lez-Alonso, J. (2019). Practical hydration solutions for sports. Nutrients, 11.","DOI":"10.3390\/nu11071550"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1745-6215-10-27","article-title":"Design, analysis, and presentation of crossover trials","volume":"10","author":"Mills","year":"2009","journal-title":"Trials"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.jpainsymman.2014.11.294","article-title":"Which treatment is better? Ascertaining patient preferences with crossover randomized controlled trials","volume":"49","author":"Hui","year":"2015","journal-title":"J. Pain Symptom Manag."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Louis, T.A., Philip, W.L., and Polansky, M. (2019). Crossover and self-controlled designs in clinical research. Medical Uses of Statistics, CRC Press.","DOI":"10.1201\/9780429187445-5"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kanda, K., Sakuma, J., Akimoto, T., Kawakami, Y., and Suzuki, K. (2017). Detection of titin fragments in urine in response to exercise-induced muscle damage. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0181623"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s40279-021-01558-y","article-title":"The hydrating effects of hypertonic, isotonic and hypotonic sports drinks and waters on central hydration during continuous exercise: A systematic meta-analysis and perspective","volume":"52","author":"Rowlands","year":"2022","journal-title":"Sports Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1139\/apnm-2013-0434","article-title":"Postexercise rehydration: Potassium-rich drinks versus water and a sports drink","volume":"39","year":"2014","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1139\/apnm-2017-0361","article-title":"Carbohydrate and protein intake during exertional heat stress ameliorates intestinal epithelial injury and small intestine permeability","volume":"42","author":"Snipe","year":"2017","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1152\/japplphysiol.01032.2018","article-title":"Impact of exercise-induced hypohydration on gastrointestinal integrity, function, symptoms, and systemic endotoxin and inflammatory profile","volume":"126","author":"Costa","year":"2019","journal-title":"J. Appl. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"G155","DOI":"10.1152\/ajpgi.00066.2012","article-title":"Physiology and pathophysiology of splanchnic hypoperfusion and intestinal injury during exercise: Strategies for evaluation and prevention","volume":"303","author":"Lenaerts","year":"2012","journal-title":"Am. J. Physiol.-Gastrointest. Liver Physiol."},{"key":"ref_23","first-page":"622","article-title":"Effect of a sports drink based on highly-branched cyclic dextrin on cytokine responses to exhaustive endurance exercise","volume":"54","author":"Suzuki","year":"2014","journal-title":"J. Sports Med. Phys. Fit."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1152\/japplphysiol.00622.2016","article-title":"Recovery of the immune system after exercise","volume":"122","author":"Peake","year":"2017","journal-title":"J. Appl. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1096\/fj.201601222R","article-title":"Treating inflammation and infection in the 21st century: New hints from decoding resolution mediators and mechanisms","volume":"31","author":"Serhan","year":"2017","journal-title":"FASEB J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1152\/japplphysiol.00745.2016","article-title":"Optimizing the restoration and maintenance of fluid balance after exercise-induced dehydration","volume":"122","author":"Evans","year":"2017","journal-title":"J. Appl. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1123\/ijsnem.2018-0047","article-title":"Sucrose and sodium but not caffeine content influence the retention of beverages in humans under euhydrated conditions","volume":"29","author":"Maughan","year":"2019","journal-title":"Int. J. Sport Nutr. Exerc. Metab."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1007\/s40279-017-0759-2","article-title":"Selected in-season nutritional strategies to enhance recovery for team sport athletes: A practical overview","volume":"47","author":"Heaton","year":"2017","journal-title":"Sports Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1002\/j.2040-4603.2014.tb00555.x","article-title":"Optimal composition of fluid-replacement beverages","volume":"4","author":"Baker","year":"2014","journal-title":"Compr. Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5527\/wjn.v7.i1.1","article-title":"Fluid balance concepts in medicine: Principles and practice","volume":"7","author":"Roumelioti","year":"2018","journal-title":"World J. Nephrol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/nrneph.2013.232","article-title":"Fluid management for the prevention and attenuation of acute kidney injury","volume":"10","author":"Prowle","year":"2014","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Orr\u00f9, S., Imperlini, E., Nigro, E., Alfieri, A., Cevenini, A., Polito, R., Daniele, A., Buono, P., and Mancini, A. (2018). Role of functional beverages on sport performance and recovery. Nutrients, 10.","DOI":"10.3390\/nu10101470"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chatterjee, A., and Abraham, J. (2019). A comprehensive study on sports and energy drinks. Sports and Energy Drinks, Woodhead Publishing.","DOI":"10.1016\/B978-0-12-815851-7.00015-2"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Can, S., and Can, H. (2025). Functional Nutritional Strategies for Peak Athletic Performance. Evaluating the Effectiveness of Functional Ingredients in Sports Nutrition, IGI Global Scientific Publishing.","DOI":"10.4018\/979-8-3693-8699-6.ch001"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1249\/JSR.0000000000000475","article-title":"Sports drinks on the edge of a new era","volume":"17","author":"Sutehall","year":"2018","journal-title":"Curr. Sports Med. Rep."},{"key":"ref_36","first-page":"63","article-title":"Effects of Hypotonic and Isotonic Sports Drinks on Endurance Performance and Physiology","volume":"14","author":"Bonetti","year":"2010","journal-title":"Sportscience"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"71","DOI":"10.12703\/r\/10-71","article-title":"Recent advances in understanding the role of IL-4 signaling","volume":"10","author":"Keegan","year":"2021","journal-title":"Fac. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/1550-2783-11-39","article-title":"Menstrual cycle phase and carbohydrate ingestion alter immune response following endurance exercise and high intensity time trial performance test under hot conditions","volume":"11","author":"Hashimoto","year":"2014","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1080\/17461391.2021.1939428","article-title":"Fluid and electrolyte balance considerations for female athletes","volume":"22","author":"Galloway","year":"2022","journal-title":"Eur. J. Sport Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.bj.2020.02.005","article-title":"Differential inflammatory response of men and women subjected to an acute resistance exercise","volume":"44","author":"Fontana","year":"2021","journal-title":"Biomed. J."},{"key":"ref_41","first-page":"310","article-title":"Crossover trials","volume":"1","author":"Kenward","year":"2014","journal-title":"Methods Appl. Stat. Clin. Trials Concepts Princ. Trials Des."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1111\/j.1469-185X.2007.00027.x","article-title":"Effect size, confidence interval and statistical significance: A practical guide for biologists","volume":"82","author":"Nakagawa","year":"2007","journal-title":"Biol. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1152\/physrev.00038.2020","article-title":"Exercise under heat stress: Thermoregulation, hydration, performance implications, and mitigation strategies","volume":"101","author":"Eijsvogels","year":"2021","journal-title":"Physiol. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1080\/23328940.2020.1826841","article-title":"Kidney physiology and pathophysiology during heat stress and the modification by exercise, dehydration, heat acclimation and aging","volume":"8","author":"Chapman","year":"2021","journal-title":"Temperature"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1007\/s00421-023-05340-y","article-title":"Modelling inter-individual variability in acute and adaptive responses to interval training: Insights into exercise intensity normalisation","volume":"124","author":"Bossi","year":"2024","journal-title":"Eur. J. Appl. Physiol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/14\/2379\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:13:06Z","timestamp":1760033586000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/14\/2379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,21]]},"references-count":45,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["nu17142379"],"URL":"https:\/\/doi.org\/10.3390\/nu17142379","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,21]]}}}