{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:03:17Z","timestamp":1792602197709,"version":"4.5.0"},"reference-count":58,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background and Objectives: Research on the impact of leucine on older sarcopenic patients is scarce, and investigations on this subject have led to contradictory findings in the literature. Our goal was to compile data from the available studies in the literature to explore the effect of leucine supplementation on parameters associated with sarcopenia in elderly individuals. Methods: The meta-analysis included older persons over 65 years of age who were recruited on the basis of the European Working Group on Sarcopenia in Older People sarcopenia criteria. Studies that were included were those in which at least one sarcopenia criterion was measured, including grip strength, appendicular skeletal muscle mass\/height2, gait speed, and the short physical performance battery index. Results: The meta-analysis included ten randomized controlled trials and one prospective study. The leucine group included 566 participants, whereas the placebo group included 567 patients. Patients receiving leucine and patients receiving a placebo had significantly different handgrip (p = 0.03), appendicular skeletal muscle mass\/height2 (p = 0.0.2), and gait speed (p = 0.008). Patients received a high dosage of leucine, and there was a significant difference in the appendicular skeletal muscle mass\/height2 (p = 0.02) and gait speed (p = 0.01) between the high dosage of the leucine group and the control group. When vitamin D was combined with leucine, the appendicular skeletal muscle mass\/height2 (p = 0.03) significantly differed between the leucine group receiving vitamin D and the control group. Conclusions: Low-quality evidence was found that older sarcopenic patients receiving leucine may show trends toward improved skeletal muscle strength, skeletal muscle quality, and physical performance. The capacity of leucine supplementation to have a beneficial therapeutic impact in older sarcopenic individuals is restricted when it is used alone without concurrent additional therapy.<\/jats:p>","DOI":"10.3390\/nu17152413","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T10:28:09Z","timestamp":1753352889000},"page":"2413","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Leucine Supplementation in Older Adults with Sarcopenia: A Meta-Analysis"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1239-9584","authenticated-orcid":false,"given":"Chienhsiu","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin Town 622401, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Hong","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin Town 622401, Taiwan"},{"name":"School of Medicine, Tzu Chi University, Hualien 97004, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1093\/ageing\/afq034","article-title":"Sarcopenia: European consensus on definition and diagnosis: Report of the European Working Group on Sarcopenia in Older People","volume":"39","author":"Baeyens","year":"2010","journal-title":"Age Ageing"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.jamda.2011.01.003","article-title":"Sarcopenia: An undiagnosed condition in older adults. Current consensus definition: Prevalence, etiology, and consequences. International working group on sarcopenia","volume":"12","author":"Fielding","year":"2011","journal-title":"J. Am. Med. Dir. Assoc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1093\/ageing\/afy169","article-title":"Sarcopenia: Revised European consensus on definition and diagnosis","volume":"48","author":"Bahat","year":"2019","journal-title":"Age Ageing"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1093\/gerona\/glx245","article-title":"Associations of sarcopenia definitions, and their components, with the incidence of recurrent falling and fractures: The Longitudinal Aging Study Amsterdam","volume":"73","author":"Schaap","year":"2018","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s40814-016-0067-x","article-title":"A feasibility study of implementing grip strength measurement into routine hospital practice (GRImP): Study protocol","volume":"2","author":"Ibrahim","year":"2016","journal-title":"Pilot Feasibility Stud."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/S0140-6736(14)62000-6","article-title":"Prognostic value of grip strength: Findings from the Prospective Urban Rural Epidemiology (PURE) study","volume":"386","author":"Leong","year":"2015","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1093\/epirev\/mxs006","article-title":"Adiposity, muscle mass, and muscle strength in relation to functional decline in older persons","volume":"35","author":"Schaap","year":"2013","journal-title":"Epidemiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2636","DOI":"10.1016\/S0140-6736(19)31138-9","article-title":"Sarcopenia","volume":"393","author":"Sayer","year":"2019","journal-title":"Lancet"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1093\/ageing\/afaa123","article-title":"The clinical usefulness of muscle mass and strength measures in older people: A systematic review","volume":"50","author":"Lunt","year":"2021","journal-title":"Age Ageing"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.maturitas.2017.04.007","article-title":"Sarcopenia as a predictor of all-cause mortality among community-dwelling older people: A systematic review and meta-analysis","volume":"103","author":"Liu","year":"2017","journal-title":"Maturitas"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1002\/jcsm.12411","article-title":"Sarcopenia and its association with falls and fractures in older adults: A systematic review and meta-analysis","volume":"10","author":"Yeung","year":"2019","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1093\/ageing\/afs197","article-title":"Prevalence of sarcopenia in community-dwelling older people in the UK using the European Working Group on Sarcopenia in Older People (EWGSOP) definition: Findings from the Hertfordshire Cohort Study (HCS)","volume":"42","author":"Patel","year":"2013","journal-title":"Age Ageing"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1093\/ageing\/afu115","article-title":"Prevalence of and interventions for sarcopenia in ageing adults: A systematic review. Report of the International Sarcopenia Initiative (EWGSOP and IWGS)","volume":"43","author":"Landi","year":"2014","journal-title":"Age Ageing"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"98","DOI":"10.4235\/agmr.19.0028","article-title":"Pharmacological interventions for treatment of sarcopenia: Current status of drug development for sarcopenia","volume":"23","author":"Kwak","year":"2019","journal-title":"Ann. Geriatr. Med. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.physio.2019.08.005","article-title":"Exercise as a treatment for sarcopenia: An umbrella review of systematic review evidence","volume":"107","author":"Moore","year":"2020","journal-title":"Physiotherapy"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1007\/s12603-019-1196-8","article-title":"Exercise interventions for the prevention and treatment of sarcopenia: A systematic umbrella review","volume":"23","author":"Delaere","year":"2019","journal-title":"J. Nutr. Health Aging"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"71","DOI":"10.22540\/JFSF-04-071","article-title":"Current practice in the diagnosis and management of sarcopenia and frailty\u2014Results from a UK-wide survey","volume":"4","author":"Offord","year":"2019","journal-title":"J. Frailty Sarcopenia Falls"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.jamda.2013.05.021","article-title":"Evidence-based recommendations for optimal dietary protein intake in older people: A position paper from the PROT-AGE Study Group","volume":"14","author":"Bauer","year":"2013","journal-title":"J. Am. Med. Dir. Assoc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"49","DOI":"10.5535\/arm.20111","article-title":"Association between length of stay in the intensive care unit and sarcopenia among hemiplegic stroke patients","volume":"45","author":"Jang","year":"2021","journal-title":"Ann. Rehabil. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"210","DOI":"10.4235\/agmr.21.0068","article-title":"Combined impact of positive screen for sarcopenia and frailty on physical function, cognition and nutrition in the community-dwelling older adult","volume":"25","author":"Lee","year":"2021","journal-title":"Ann. Geriatr. Med. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1093\/gerona\/glw017","article-title":"Sarcopenia is associated with lower skeletal muscle capillarization and exercise capacity in older adults","volume":"71","author":"Prior","year":"2016","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.mcna.2011.02.008","article-title":"Treatment strategies for sarcopenia and frailty","volume":"95","author":"Rolland","year":"2011","journal-title":"Med. Clin. N. Am."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Ruggiero, M., Motti, M.L., Meccariello, R., and Mazzeo, F. (2025). Resveratrol and physical activity: A winning combination for maintaining health and well-being?. Nutrients, 17.","DOI":"10.3390\/nu17050837"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1113\/jphysiol.2006.110742","article-title":"Leucine supplementation improves muscle protein synthesis in elderly men independently of hyperaminoacidaemia","volume":"575","author":"Rieu","year":"2006","journal-title":"J. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.clnu.2012.01.005","article-title":"Leucine supplementation chronically improves muscle protein synthesis in older adults consuming the RDA for protein","volume":"31","author":"Casperson","year":"2012","journal-title":"Clin. Nutr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/nure.12019","article-title":"Nutritional strategies to attenuate muscle disuse atrophy","volume":"71","author":"Wall","year":"2013","journal-title":"Nutr. Rev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"625","DOI":"10.2337\/diacare.26.3.625","article-title":"Amino acid ingestion strongly enhances insulin secretion in patients with long-term type 2 diabetes","volume":"26","author":"Kruijshoop","year":"2003","journal-title":"Diabetes Care"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2616S","DOI":"10.3945\/jn.116.234518","article-title":"Leucine and mammalian target of rapamycin-dependent activation of muscle protein synthesis in aging","volume":"146","year":"2016","journal-title":"J. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Arnau, F.M., Fonfr\u00eda-Vivas, R., and Cauli, O. (2019). Beneficial effects of leucine supplementation on criteria for sarcopenia: A systematic review. Nutrients, 11.","DOI":"10.3390\/nu11102504"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.nut.2018.05.028","article-title":"Effects of a leucine-enriched amino acid supplement on muscle mass, muscle strength, and physical function in post-stroke patients with sarcopenia: A randomized controlled trial","volume":"58","author":"Yoshimura","year":"2019","journal-title":"Nutrition"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Arnau, F.M., Fonfr\u00eda-Vivas, R., Buigues, C., Castillo, Y., Molina, P., Hoogland, A.J., van Doesburg, F., Pruimboom, L., Fern\u00e1ndez-Garrido, J., and Cauli, O. (2020). Effects of leucine administration in sarcopenia: A randomized and placebo-controlled clinical trial. Nutrients, 12.","DOI":"10.3390\/nu12040932"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1016\/j.jamda.2015.05.021","article-title":"Effects of a vitamin D and leucine-enriched whey protein nutritional supplement on measures of sarcopenia in older adults, the PROVIDE study: A randomized, double-blind, placebo-controlled trial","volume":"16","author":"Bauer","year":"2015","journal-title":"J. Am. Med. Dir. Assoc."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Amasene, M., Besga, A., Echeverria, I., Urquiza, M., Ruiz, J.R., Rodriguez-Larrad, A., Aldamiz, M., Anaut, P., Irazusta, J., and Labayen, I. (2019). Effects of leucine-enriched whey protein supplementation on physical function in post-hospitalized older adults participating in 12-weeks of resistance training program: A randomized controlled trial. Nutrients, 11.","DOI":"10.3390\/nu11102337"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"LACE Study Group, Achison, M., Adamson, S., Akpan, A., Aspray, T., Avenell, A., Band, M.M., Bashir, T., Burton, L.A., and Cvoro, V. (2022). Effect of perindopril or leucine on physical performance in older people with sarcopenia: The LACE randomized controlled trial. J. Cachexia Sarcopenia Muscle, 13, 858\u2013871.","DOI":"10.1002\/jcsm.12934"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1007\/s12603-022-1853-1","article-title":"De-training effects following leucine-enriched whey protein supplementation and resistance training in older adults with sarcopenia: A randomized controlled trial with 24 weeks of follow-up","volume":"26","author":"Mori","year":"2022","journal-title":"J. Nutr. Health Aging"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Park, S., Chae, M., Park, H., and Park, K. (2021). Higher branched-chain amino acid intake is associated with handgrip strength among Korean older adults. Nutrients, 13.","DOI":"10.3390\/nu13051522"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.nut.2018.09.031","article-title":"Vitamin D, muscle recovery, sarcopenia, cachexia, and muscle atrophy","volume":"60","author":"Garcia","year":"2019","journal-title":"Nutrition"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1210\/jc.2014-3519","article-title":"Vitamin D and physical activity status: Associations with five-year changes in body composition and muscle function in community-dwelling older adults","volume":"100","author":"Scott","year":"2015","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Uchitomi, R., Oyabu, M., and Kamei, Y. (2020). Vitamin D and sarcopenia: Potential of vitamin D supplementation in sarcopenia prevention and treatment. Nutrients, 12.","DOI":"10.3390\/nu12103189"},{"key":"ref_40","first-page":"ED000142","article-title":"Updated guidance for trusted systematic reviews: A new edition of the Cochrane Handbook for Systematic Reviews of Interventions","volume":"10","author":"Cumpston","year":"2019","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Guo, Y., Fu, X., Hu, Q., Chen, L., and Zuo, H. (2022). The effect of leucine supplementation on sarcopenia-related measures in older adults: A systematic review and meta-analysis of 17 randomized controlled trials. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.929891"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Atkins, D., Eccles, M., Flottorp, S., Guyatt, G.H., Henry, D., Hill, S., Liberati, A., O\u2019Connell, D., Oxman, A.D., and Phillips, B. (2004). Systems for grading the quality of evidence and the strength of recommendations I: Critical appraisal of existing approaches The GRADE Working Group. BMC Health Serv. Res., 4.","DOI":"10.1186\/1472-6963-4-38"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1136\/bmj.39489.470347.AD","article-title":"GRADE: An emerging consensus on rating quality of evidence and strength of recommendations","volume":"336","author":"Guyatt","year":"2008","journal-title":"BMJ"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.clnu.2017.01.005","article-title":"Sufficient levels of 25-hydroxyvitamin D and protein intake required to increase muscle mass in sarcopenic older adults\u2014The PROVIDE study","volume":"37","author":"Verlaan","year":"2018","journal-title":"Clin. Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1532-5415.2011.03776.x","article-title":"Effects of exercise and amino acid supplementation on body composition and physical function in community-dwelling elderly Japanese sarcopenic women: A randomized controlled trial","volume":"60","author":"Kim","year":"2012","journal-title":"J. Am. Geriatr. Soc."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1093\/ajcn\/nqaa449","article-title":"Does supplementation with leucine-enriched protein alone and in combination with fish-oil-derived n-3 PUFA affect muscle mass, strength, physical performance, and muscle protein synthesis in well-nourished older adults? A randomized, double-blind, placebo-controlled trial","volume":"113","author":"Murphy","year":"2021","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"830","DOI":"10.3945\/ajcn.115.113357","article-title":"Whey protein, amino acids, and vitamin D supplementation with physical activity increases fat-free mass and strength, functionality, and quality of life and decreases inflammation in sarcopenic elderly","volume":"103","author":"Rondanelli","year":"2016","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1002\/jcsm.12532","article-title":"Improving rehabilitation in sarcopenia: A randomized-controlled trial utilizing a muscle-targeted food for special medical purposes","volume":"11","author":"Rondanelli","year":"2020","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Rondanelli, M., Gasparri, C., Barrile, G.C., Battaglia, S., Cavioni, A., Giusti, R., Mansueto, F., Moroni, A., Nannipieri, F., and Patelli, Z. (2022). Effectiveness of a novel food composed of leucine, omega-3 fatty acids and probiotic Lactobacillus paracasei PS23 for the treatment of sarcopenia in elderly subjects: A 2-month randomized double-blind placebo-controlled trial. Nutrients, 14.","DOI":"10.3390\/nu14214566"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/j.clnu.2020.08.017","article-title":"Effects of adequate dietary protein with whey protein, leucine, and vitamin D supplementation on sarcopenia in older adults: An open-label, parallel-group study","volume":"40","author":"Lin","year":"2021","journal-title":"Clin. Nutr."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Chang, M.C., and Choo, Y.J. (2023). Effects of whey protein, leucine, and vitamin D supplementation in patients with sarcopenia: A systematic review and meta-analysis. Nutrients, 15.","DOI":"10.3390\/nu15030521"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"104758","DOI":"10.1016\/j.archger.2022.104758","article-title":"Effects of leucine-rich protein supplements in older adults with sarcopenia: A systematic review and meta-analysis of randomized controlled trials","volume":"102","author":"Lee","year":"2022","journal-title":"Arch. Gerontol. Geriatr."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/j.clnu.2014.04.007","article-title":"Protein intake and exercise for optimal muscle function with aging: Recommendations from the ESPEN Expert Group","volume":"33","author":"Deutz","year":"2014","journal-title":"Clin. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Rondanelli, M., Nichetti, M., Peroni, G., Faliva, M.A., Naso, M., Gasparri, C., Perna, S., Oberto, L., Di Paolo, E., and Riva, A. (2021). Where to find leucine in food and how to feed elderly with sarcopenia in order to counteract loss of muscle mass: Practical advice. Front. Nutr., 7.","DOI":"10.3389\/fnut.2020.622391"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.jamda.2010.04.014","article-title":"Nutritional recommendations for the management of sarcopenia","volume":"11","author":"Morley","year":"2010","journal-title":"J. Am. Med. Dir. Assoc."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1093\/ajcn\/nqaa323","article-title":"Dietary leucine requirement of older men and women is higher than current recommendations","volume":"113","author":"Szwiega","year":"2021","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.maturitas.2021.01.002","article-title":"The effect of vitamin D plus protein supplementation on sarcopenia: A systematic review and meta-analysis of randomized controlled trials","volume":"145","author":"Gkekas","year":"2021","journal-title":"Maturitas"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1002\/jcsm.12976","article-title":"Effect of vitamin D monotherapy on indices of sarcopenia in community-dwelling older adults: A systematic review and meta-analysis","volume":"13","author":"Prokopidis","year":"2022","journal-title":"J. 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