{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T04:31:33Z","timestamp":1789792293408,"version":"4.0.1"},"reference-count":51,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gyeonggi Province","award":["D2403010#"],"award-info":[{"award-number":["D2403010#"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background\/Objectives: Green tripe (GRET) is rich in essential fatty acids, vitamins, calcium, phosphorus, and other nutrients and contains various beneficial microorganisms, including lactobacillus, along with feed components consumed by ruminants. Methods: In this study, Latilactobacillus sakei and L. curvatus were isolated from GRET and evaluated for their potential as probiotics, focusing on their anti-inflammatory properties and ability to modulate inflammatory responses. Results: When heat-killed L. sakei or L. curvatus (108 CFU\/mL) and their metabolites (0.5 mg\/mL) were applied to RAW 264.7 macrophages stimulated with LPS, nitric oxide (NO) production was reduced by approximately 10\u201335% and 2\u201311%, respectively. Furthermore, the expression levels of key anti-inflammatory cytokines, TNF-\u03b1 and IL-6, were suppressed by more than 5%. These effects were not due to cytotoxicity but instead due to genuine anti-inflammatory activity. In addition, both strains exhibited antioxidant activity, as demonstrated by their performance in ABTS and FRAP assays. Conclusions: These findings suggest that L. sakei and L. curvatus have significant antioxidant and anti-inflammatory properties, highlighting their potential as probiotics and prebiotics. Moreover, these newly isolated strains from GRET are expected to serve as valuable functional ingredients for developing health-promoting foods and dietary supplements.<\/jats:p>","DOI":"10.3390\/nu17152464","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T17:06:01Z","timestamp":1753722361000},"page":"2464","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antioxidant and Anti-Inflammatory Activities of Latilactobacillus curvatus and L. sakei Isolated from Green Tripe"],"prefix":"10.3390","volume":"17","author":[{"given":"Ga Hun","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Life Sciences, College of Bionano, Gachon University, Seongnamdaero 1342, Seongnam-si 461-701, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Hyun","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, College of Bionano, Gachon University, Seongnamdaero 1342, Seongnam-si 461-701, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, College of Bionano, Gachon University, Seongnamdaero 1342, Seongnam-si 461-701, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Kweon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, College of Bionano, Gachon University, Seongnamdaero 1342, Seongnam-si 461-701, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.fsi.2017.06.017","article-title":"Apple cider vinegar boosted immunomodulatory and health promoting effects of Lactobacillus casei in common carp (Cyprinus carpio)","volume":"67","author":"Safari","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Garcia-Gonzalez, N., Battista, N., Prete, R., and Corsetti, A. (2021). Health-promoting role of Lactiplantibacillus plantarum isolated from fermented foods. Microorganisms, 9.","DOI":"10.3390\/microorganisms9020349"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"106614","DOI":"10.1016\/j.micpath.2024.106614","article-title":"Acanthopanax senticosus cultures fermented by Lactobacillus rhamnosus enhanced immune response through improvement of antioxidant activity and inflammation in crucian carp (Carassius auratus)","volume":"190","author":"Ma","year":"2024","journal-title":"Microb. Pathog."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1002\/fsn3.2145","article-title":"The effect of yogurt fortified with Lactobacillus acidophilus and Bifidobacterium sp. probiotic in patients with lactose intolerance","volume":"9","author":"Masoumi","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.3389\/fmicb.2018.01953","article-title":"Probiotic Lactobacillus plantarum promotes intestinal barrier function by strengthening the epithelium and modulating gut microbiota","volume":"9","author":"Wang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Mu, Q., Tavella, V.J., and Luo, X.M. (2018). Role of Lactobacillus reuteri in human health and diseases. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.00757"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Blackwood, B.P., Yuan, C.Y., Wood, D.R., Nicolas, J.D., Grothaus, J.S., and Hunter, C.J. (2017). Probiotic Lactobacillus species strengthen intestinal barrier function and tight junction integrity in experimental necrotizing enterocolitis. J. Probiotics Health, 5.","DOI":"10.4172\/2329-8901.1000159"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Villena, J., Chiba, E., Tomosada, Y., Salva, S., Marranzino, G., Kitazawa, H., and Alvarez, S. (2012). Orally administered Lactobacillus rhamnosus modulates the respiratory immune response triggered by the viral pathogen-associated molecular pattern poly(I:C). BMC Immunol., 13.","DOI":"10.1186\/1471-2172-13-53"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Rastogi, S., and Singh, A. (2022). Gut microbiome and human health: Exploring how the probiotic genus Lactobacillus modulate immune responses. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.1042189"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"551","DOI":"10.5851\/kosfa.2015.35.4.551","article-title":"Characterization of selected Lactobacillus strains for use as probiotics","volume":"35","author":"Song","year":"2015","journal-title":"Korean J. Food Sci. Anim. Resour."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Bidossi, A., De Grandi, R., Toscano, M., Bottagisio, M., De Vecchi, E., Gelardi, M., and Drago, L. (2018). Probiotics Streptococcus salivarius 24SMB and Streptococcus oralis 89a interfere with biofilm formation of pathogens of the upper respiratory tract. BMC Infect. Dis., 18.","DOI":"10.1186\/s12879-018-3576-9"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-L\u00f3pez, A., Camelo-Castillo, A., Ferrer, M.D., Simon-Soro, \u00c1., and Mira, A. (2017). Health-associated niche inhabitants as oral probiotics: The case of Streptococcus dentisani. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00379"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1097\/MCG.0000000000001170","article-title":"Thirty years of Lactobacillus rhamnosus GG: A review","volume":"53","author":"Capurso","year":"2019","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4652","DOI":"10.3748\/wjg.v24.i41.4652","article-title":"Mucosal adhesion and anti-inflammatory effects of Lactobacillus rhamnosus GG in the human colonic mucosa: A proof-of-concept study","volume":"24","author":"Pagnini","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"9632","DOI":"10.1039\/D1FO00277E","article-title":"Exopolysaccharides produced by Lactobacillus rhamnosus GG alleviate hydrogen peroxide-induced intestinal oxidative damage and apoptosis through the Keap1\/Nrf2 and Bax\/Bcl-2 pathways in vitro","volume":"12","author":"Li","year":"2021","journal-title":"Food Funct."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Vougiouklaki, D., Tsironi, T., Tsantes, A.G., Tsakali, E., Van Impe, J.F., and Houhoula, D. (2023). Probiotic properties and antioxidant activity in vitro of lactic acid bacteria. Microorganisms, 11.","DOI":"10.3390\/microorganisms11051264"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2128","DOI":"10.1016\/j.lfs.2007.03.018","article-title":"Enhancement of gastric mucosal integrity by Lactobacillus rhamnosus GG","volume":"80","author":"Lam","year":"2007","journal-title":"Life Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1016\/j.clnu.2016.09.025","article-title":"Lactobacillus rhamnosus GG treatment improves intestinal permeability and modulates inflammatory response and homeostasis of spleen and colon in experimental model of Pseudomonas aeruginosa pneumonia","volume":"36","author":"Khailova","year":"2017","journal-title":"Clin. Nutr."},{"key":"ref_19","first-page":"80","article-title":"Rumen content as animal feed: A review","volume":"7","author":"Elfaki","year":"2016","journal-title":"J. Vet. Med. Anim. Prod."},{"key":"ref_20","first-page":"580","article-title":"Studies on nutrients and anti-nutrients of rumen digesta from three most domesticated ruminants in Nigeria","volume":"11","author":"Agbabiaka","year":"2012","journal-title":"Pak. J. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15232\/S1080-7446(15)30076-0","article-title":"The rumen microbiome: Composition, abundance, diversity, and new investigative tools","volume":"30","author":"Ossa","year":"2014","journal-title":"Prof. Anim. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Jami, E., and Mizrahi, I. (2012). Composition and similarity of bovine rumen microbiota across individual animals. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0033306"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"198","DOI":"10.3390\/microorganisms3020198","article-title":"Comparison of microbiological and probiotic characteristics of lactobacilli isolates from dairy food products and animal rumen contents","volume":"3","author":"Jose","year":"2015","journal-title":"Microorganisms"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.1128\/JB.01618-09","article-title":"Physiological and transcriptional response of Lactobacillus casei ATCC 334 to acid stress","volume":"192","author":"Broadbent","year":"2010","journal-title":"J. Bacteriol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1177\/0394632015590948","article-title":"Evaluation of resistance to low pH and bile salts of human Lactobacillus spp. isolates","volume":"28","author":"Fuochi","year":"2015","journal-title":"Int. J. Immunopathol. Pharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5252","DOI":"10.1038\/s41467-019-13118-0","article-title":"Characterization of antibiotic resistance genes in the species of the rumen microbiota","volume":"10","author":"Sabino","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hu, G., Jiang, H., Zong, Y., Datsomor, O., Kou, L., An, Y., Zhao, J., and Miao, L. (2022). Characterization of lactic acid-producing bacteria isolated from rumen: Growth, acid and bile salt tolerance, and antimicrobial function. Fermentation, 8.","DOI":"10.3390\/fermentation8080385"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ladha, G., and Jeevaratnam, K. (2020). A novel antibacterial compound produced by Lactobacillus plantarum LJR13 isolated from rumen liquor of goat effectively controls multi-drug resistant human pathogens. Microbiol. Res., 241.","DOI":"10.1016\/j.micres.2020.126563"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1007\/s12602-023-10139-6","article-title":"Heat-killed Latilactobacillus sakei CNSC001WB and Lactobacillus pentosus WB693 have an anti-inflammatory effect on LPS-stimulated RAW 264.7 cells","volume":"16","author":"Jung","year":"2024","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.intimp.2015.05.037","article-title":"Lactobacillus sakei K040706 evokes immunostimulatory effects on macrophages through TLR 2-mediated activation","volume":"28","author":"Jung","year":"2015","journal-title":"Int. Immunopharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"358","DOI":"10.4014\/jmb.2309.09007","article-title":"Heat-treated paraprobiotic Latilactobacillus sakei KU15041 and Latilactobacillus curvatus KU15003 show an antioxidant and immunostimulatory effect","volume":"34","author":"Hyun","year":"2023","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Zagorec, M., and Champomier-Verg\u00e8s, M.C. (2017). Lactobacillus sakei: A starter for sausage fermentation, a protective culture for meat products. Microorganisms, 5.","DOI":"10.3390\/microorganisms5030056"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.ijfoodmicro.2018.04.030","article-title":"Assertiveness of Lactobacillus sakei and Lactobacillus curvatus in a fermented sausage model","volume":"285","author":"Eisenbach","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s12602-017-9301-y","article-title":"Probiotic characteristics of Lactobacillus curvatus DN317, a strain isolated from chicken ceca","volume":"9","author":"Zommiti","year":"2017","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Castilho, N.P.A., Colombo, M., Oliveira LLde Todorov, S.D., and Nero, L.A. (2019). Lactobacillus curvatus UFV-NPAC1 and other lactic acid bacteria isolated from calabresa, a fermented meat product, present high bacteriocinogenic activity against Listeria monocytogenes. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1436-4"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"105446","DOI":"10.1016\/j.micpath.2022.105446","article-title":"Antibacterial and anti-biofilm activities of probiotic Lactobacillus curvatus BSF206 and Pediococcus pentosaceus AC1-2 against Streptococcus mutans","volume":"164","author":"Luan","year":"2022","journal-title":"Microb. Pathog."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.ijbiomac.2022.04.203","article-title":"Characterization on structure and bioactivities of an exopolysaccharide from Lactobacillus curvatus SJTUF 62116","volume":"210","author":"Zhu","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Melian, C., Ploper, D., Cheh\u00edn, R., Vignolo, G., and Castellano, P. (2024). Impairment of Listeria monocytogenes biofilm developed on industrial surfaces by Latilactobacillus curvatus CRL1579 bacteriocin. Food Microbiol., 121.","DOI":"10.1016\/j.fm.2024.104491"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"184","DOI":"10.17642\/jcc.29.4.2","article-title":"Antifungal effect of chitosan hydrolysates against ginseng infectious fungi Fusarium foetens","volume":"29","author":"Lee","year":"2024","journal-title":"J. Chitin Chitosan"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.17642\/jcc.28.1.1","article-title":"Effects of chitosan as a growth regulator of the mealworm Tenebrio molitor","volume":"28","author":"Park","year":"2023","journal-title":"J. Chitin Chitosan"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.intimp.2009.12.011","article-title":"Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens","volume":"10","author":"Na","year":"2010","journal-title":"Int. Immunopharmacol."},{"key":"ref_42","first-page":"87","article-title":"Isolation and characterization of a bacterial isolate for polyhydroxyalkanoates production","volume":"19","author":"Kim","year":"2014","journal-title":"J. Chitin Chitosan"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/s00449-015-1514-4","article-title":"Isolation and characterization of a serine protease-producing marine bacterium Marinomonas arctica PT-1","volume":"39","author":"Yoo","year":"2016","journal-title":"Bioprocess Biosyst. Eng."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Avelelas, F., Horta, A., Pinto, L.F., Cotrim Marques, S., Marques Nunes, P., Pedrosa, R., and Leandro, S.M. (2019). Antifungal and antioxidant properties of chitosan polymers obtained from nontraditional Polybius henslowii sources. Mar. Drugs, 17.","DOI":"10.3390\/md17040239"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1016\/j.bmc.2004.11.002","article-title":"Antioxidant activity of differently regioselective chitosan sulfates in vitro","volume":"13","author":"Xing","year":"2005","journal-title":"Bioorg. Med. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3390\/jfb6010033","article-title":"Anticancer and anti-inflammatory properties of chitin and chitosan oligosaccharides","volume":"6","author":"Azuma","year":"2015","journal-title":"J. Funct. Biomater."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s00011-022-01584-0","article-title":"Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: A systematic review and meta-analysis","volume":"71","author":"Facchin","year":"2022","journal-title":"Inflamm. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"638","DOI":"10.4014\/jmb.2201.01015","article-title":"Immunostimulatory effect of heat-killed probiotics on RAW264.7 macrophages","volume":"32","author":"Noh","year":"2022","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.antiviral.2007.03.013","article-title":"Interactions of macrophages with probiotic bacteria lead to increased antiviral response against vesicular stomatitis virus","volume":"75","author":"Ivec","year":"2007","journal-title":"Antivir. Res."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Li, H., Chen, C., Li, Y., Li, Z., Li, C., and Luan, C. (2023). Antioxidant effects and probiotic properties of Latilactobacillus sakei MS103 isolated from sweet pickled garlic. Foods, 12.","DOI":"10.3390\/foods12234276"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e20200105","DOI":"10.1590\/0001-3765202020200105","article-title":"Antioxidant activity of Lactobacillus plantarum, Lactobacillus sakei and Lactobacillus curvatus strains isolated from fermented Turkish Sucuk","volume":"92","author":"Duz","year":"2020","journal-title":"An. Acad. Bras. Ci\u00eanc."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/15\/2464\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:17:36Z","timestamp":1760033856000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/15\/2464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,28]]},"references-count":51,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["nu17152464"],"URL":"https:\/\/doi.org\/10.3390\/nu17152464","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,28]]}}}