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As such, nutraceuticals offer a compelling strategy to improve metabolic health through dietary means, especially for elderly persons who are unable to adhere to common therapeutic interventions. To address this, we examined the effects of supplementing aged sedentary rats with an MFGM\/EV-rich concentrate. Methods\/Results: In a 25-week study, 89-week-old male rats received either a milk sphingolipid-rich MFGM\/EV concentrate or a control supplement. Analysis of metabolic health using a battery of tests, including MSALL lipidomics of plasma, liver, and other peripheral tissues, revealed that MFGM\/EV supplementation promotes accretion of unique sphingolipid signatures, ameliorates ceramide biomarkers predictive of cardiovascular death, and has a general lipid-lowering effect. At the functional level, we find that these health-promoting effects are linked to increased lipoprotein particle turnover, showcased by reduced levels of triglyceride-rich particles, as well as a metabolically healthier liver, assessed using whole-body lipidomic flux analysis. Conclusions: Altogether, our work unveils that MFGM\/EV-containing food holds a potential for ameliorating age-related metabolic dysfunction in elderly individuals.<\/jats:p>","DOI":"10.3390\/nu17152529","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T08:51:41Z","timestamp":1754038301000},"page":"2529","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dietary Intake of a Milk Sphingolipid-Rich MFGM\/EV Concentrate Ameliorates Age-Related Metabolic Dysfunction"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3947-1606","authenticated-orcid":false,"given":"Richard","family":"Sprenger","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, 5230 Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8769-6968","authenticated-orcid":false,"given":"Kat","family":"Kiilerich","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, 5230 Odense, Denmark"},{"name":"Neurobiology Research Unit, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6014-2084","authenticated-orcid":false,"given":"Mikael","family":"Palner","sequence":"additional","affiliation":[{"name":"Neurobiology Research Unit, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ars\u00eanio","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CNRS, INSERM, l\u2019institut du thorax, 44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika\u00ebl","family":"Croyal","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CNRS, INSERM, l\u2019institut du thorax, 44000 Nantes, France"},{"name":"Nantes Universit\u00e9, CHU Nantes, Inserm, CNRS, SFR Sant\u00e9, Inserm UMS 016, CNRS UMS 3556, 44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Ostenfeld","sequence":"additional","affiliation":[{"name":"Arla Foods Ingredients Group P\/S, 8260 Viby J, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0535-9108","authenticated-orcid":false,"given":"Ann","family":"Bj\u00f8rnshave","sequence":"additional","affiliation":[{"name":"Arla Foods Ingredients Group P\/S, 8260 Viby J, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gitte","family":"Knudsen","sequence":"additional","affiliation":[{"name":"Neurobiology Research Unit, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4963-0276","authenticated-orcid":false,"given":"Christer","family":"Ejsing","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, 5230 Odense, Denmark"},{"name":"Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,31]]},"reference":[{"key":"ref_1","unstructured":"GBD 2021 Demographics Collaborators (2024). 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