{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:42:07Z","timestamp":1765806127633,"version":"build-2065373602"},"reference-count":62,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T00:00:00Z","timestamp":1754697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Technology Innovation Leading Talents of Guangdong Special Support Plan","award":["2021TX06N107","2018YFC1603101"],"award-info":[{"award-number":["2021TX06N107","2018YFC1603101"]}]},{"DOI":"10.13039\/501100012166","name":"the National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021TX06N107","2018YFC1603101"],"award-info":[{"award-number":["2021TX06N107","2018YFC1603101"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Objectives: Deoxynivalenol (DON) is a ubiquitous mycotoxin detected in the environment and foodstuffs. DON exposure can lead to chronic intestinal inflammation. Therefore, intervention strategy needs to be established to prevent the intestinal inflammation caused by DON. Methods: The structure of Mesona chinensis Benth polysaccharide-3 (MCP-3), a major component isolated and purified from crude MCP, was analyzed using spectroscopic and chromatographic methods. In vitro assays were conducted on the potential antioxidant bioactivities of MCP-3 and its ameliorative effects on deoxynivalenol-induced oxidative stress in intestinal epithelial cells. Results: Saline-eluted MCP-3 was identified as an acidic heterogeneous polysaccharide with an average molecular weight of 16.014 kDa. Its major monosaccharide components were glucose (20.19%), galactose (11.82%), rhamnose (17.23%), galacturonic acid (29.72%), arabinose (7.11%), xylose (8.09%), mannose (2.79%), and glucuronic acid (3.04%). The main backbone of MCP-3 was composed of the following sequence: \u21924)-\u03b1-D-GalpA-6-(1\u21924)-\u03b1-GalpA-(1\u21924)-\u03b1-D-GalpA-6-(1\u21922)-\u03b1-L-Rhap-(1\u21924)-\u03b1-D-GalpA-6-(1\u21922,4)-\u03b1-L-Rhap-(1\u2192. MCP-3 showed strong antioxidant ability in in vitro assays. It effectively prevented redox imbalance induced by the mycotoxin deoxynivalenol in intestinal epithelial cell models based on Caco-2 and NCM460 cells. MCP-3 significantly increased (p &lt; 0.05) the activities of superoxide dismutase, glutathione peroxidase, and catalase, and significantly decreased (p &lt; 0.05) the levels of malondialdehyde and reactive oxygen species, thereby improving redox homeostasis. Conclusions: MCP-3 has potential as a natural antioxidant for use in functional food and nutraceutical industries to help regulate intestinal oxidative stress caused by mycotoxin DON.<\/jats:p>","DOI":"10.3390\/nu17162592","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T09:04:53Z","timestamp":1754903093000},"page":"2592","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Structural Characterization and Ameliorative Effects of Mesona chinensis Benth Polysaccharide Against Deoxynivalenol-Induced Oxidative Stress in Intestinal Epithelial Cells"],"prefix":"10.3390","volume":"17","author":[{"given":"Ai-Hua","family":"Zhong","sequence":"first","affiliation":[{"name":"Food Safety and Health Research Center, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong-Hongkong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China"},{"name":"Public Health Service Center, Bao\u2019an District, Shenzhen 518126, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiu-Yun","family":"Li","sequence":"additional","affiliation":[{"name":"Food Safety and Health Research Center, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong-Hongkong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Su","sequence":"additional","affiliation":[{"name":"Food Safety and Health Research Center, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong-Hongkong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Jun","family":"Huang","sequence":"additional","affiliation":[{"name":"Food Safety and Health Research Center, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong-Hongkong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangzhou Center for Food and Drug Evaluation, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Dan","family":"Wang","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science (2019RU014), China National Center for Food Safety Risk Assessment, Beijing 100022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Song","sequence":"additional","affiliation":[{"name":"Food Safety and Health Research Center, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong-Hongkong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6430-1302","authenticated-orcid":false,"given":"Yong-Ning","family":"Wu","sequence":"additional","affiliation":[{"name":"Food Safety and Health Research Center, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong-Hongkong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China"},{"name":"NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science (2019RU014), China National Center for Food Safety Risk Assessment, Beijing 100022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing-Fen","family":"Yang","sequence":"additional","affiliation":[{"name":"Food Safety and Health Research Center, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong-Hongkong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6611-6925","authenticated-orcid":false,"given":"Wei-Liang","family":"Wu","sequence":"additional","affiliation":[{"name":"Food Safety and Health Research Center, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong-Hongkong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"127257","DOI":"10.1016\/j.foodchem.2020.127257","article-title":"Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-alpha expression","volume":"330","author":"Huo","year":"2020","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1080\/17474124.2017.1343143","article-title":"The intestinal barrier: A fundamental role in health and 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