{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:12:17Z","timestamp":1792789937901,"version":"4.5.0"},"reference-count":35,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T00:00:00Z","timestamp":1755129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Basic Science Research Program","award":["2022R1C1C1011770"],"award-info":[{"award-number":["2022R1C1C1011770"]}]},{"name":"Basic Science Research Program","award":["RS-2023-00247455"],"award-info":[{"award-number":["RS-2023-00247455"]}]},{"name":"Basic Science Research Program","award":["RS-2024-00463250"],"award-info":[{"award-number":["RS-2024-00463250"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background\/Objectives: Sarcopenic obesity, defined by the coexistence of excessive fat accumulation and progressive muscle loss, is associated with an increased risk of metabolic dysfunction and physical disability. While 5,7-dimethoxyflavone (DMF), a bioactive flavone derived from Kaempferia parviflora, has demonstrated anti-obesity and muscle-preserving properties, its effects on sarcopenic obesity remain unclear. Methods: Four-week-old male C57BL\/6J mice were fed a high-fat diet (HFD) for 6 weeks to induce sarcopenic obesity, followed by 8 weeks of continued HFD with the oral administration of DMF. Muscle function was assessed through grip strength and treadmill running tests, while muscle and fat volumes were measured using micro-CT. Mechanistic analyses were performed using gene expression and Western blot analysis. Results: DMF significantly reduced body weight, fat mass, and adipocyte size while enhancing grip strength, endurance, skeletal muscle mass, and the muscle fiber cross-sectional area. In the gastrocnemius muscle, DMF increased the gene expression of peroxisome proliferator-activated receptor gamma coactivator-1\u03b1 (Ppargc1a) and its isoform Ppargc1a4, thereby promoting mitochondrial biogenesis. It also improved protein turnover by modulating protein synthesis and degradation via the phosphatidylinositol 3-kinase\/protein kinase B\/mechanistic target of rapamycin signaling pathway. In subcutaneous and brown adipose tissues, DMF increased mitochondrial DNA content and the expression of thermogenic and beige adipocyte-related genes. These findings suggest that DMF alleviates sarcopenic obesity by improving mitochondrial function and regulating energy metabolism in both skeletal muscle and adipose tissues via PGC-1\u03b1-mediated pathways. Thus, DMF represents a promising therapeutic candidate for the integrated management of sarcopenic obesity.<\/jats:p>","DOI":"10.3390\/nu17162642","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T15:16:37Z","timestamp":1755184597000},"page":"2642","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["5,7-Dimethoxyflavone Attenuates Sarcopenic Obesity by Enhancing PGC-1\u03b1\u2013Mediated Mitochondrial Function in High-Fat-Diet-Induced Obese Mice"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5661-2773","authenticated-orcid":false,"given":"Changhee","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2598-6066","authenticated-orcid":false,"given":"Mi-Bo","family":"Kim","sequence":"additional","affiliation":[{"name":"Graduate Program in Bioindustrial Engineering, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea"},{"name":"Department of Food Science and Nutrition, College of Fisheries Science, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8127-8132","authenticated-orcid":false,"given":"Sanggil","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, College of Fisheries Science, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Kwan","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea"},{"name":"Graduate Program in Bioindustrial Engineering, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1089\/jmf.2016.3800","article-title":"5,7-Dimethoxyflavone Attenuates Obesity by Inhibiting Adipogenesis in 3T3-L1 Adipocytes and High-Fat Diet-Induced Obese C57BL\/6J Mice","volume":"19","author":"Song","year":"2016","journal-title":"J. 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