{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:34:29Z","timestamp":1792017269245,"version":"4.4.0"},"reference-count":53,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cLegumeGEneticREsources as a tool for the development of innovative and sustainable food TEchnological system\u201d supported under the \u201cThought for Food\u201d Initiative by the Agropolis Foundation (through the \u201cInvestissementsd\u2019avenir\u201d programme","award":["ANR-10-LABX-0001-01"],"award-info":[{"award-number":["ANR-10-LABX-0001-01"]}]},{"name":"Fondazione Cariplo","award":["ANR-10-LABX-0001-01"],"award-info":[{"award-number":["ANR-10-LABX-0001-01"]}]},{"name":"Daniel and Nina Carasso Foundation","award":["ANR-10-LABX-0001-01"],"award-info":[{"award-number":["ANR-10-LABX-0001-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background\/Objectives: Unhealthy nutrition and lifestyles contribute to the development of chronic diseases such as cardiovascular disease, type 2 diabetes, and colorectal cancer. The Western diet can impair gastrointestinal motility and function. The underlying mechanisms that lead to changes in the mucus barrier and mucin profiles in response to these dietary patterns are still being studied. In mice, dietary fiber intake can improve the intestinal mucosal barrier function, enhance the differentiation process of goblet cells, and increase acidic mucin production. Our study aimed to investigate the effects of a high-fat diet (HFD) on colonic mucin expression and to assess whether chickpea accessions, known for their nutritional benefits, can mitigate these adverse effects. Methods: We investigated the effects of an HFD and an HFD associated with two accessions of chickpeas (HFD + MG_13; HFD + PI358934) on the mucin expression in murine colons of mice by conventional histochemistry and lectin-histochemistry, combined with chemical treatment and enzymatic digestion and immunohistochemistry. We evaluated possible alterations of Muc2, the main mucin secreted by the mucous cells of the colon. Results: HFD significantly reduced the expression of the mucin Muc2 and altered its composition in the colon. Compared to the CTRL group, distal and proximal measurements for HFD + PI, respectively, showed reductions of 78% and 36%; for the distal colon, a reduction of 34% was also observed for both the HFD and HFD + MG_13 diets. Changes in mucin glycosylation, including sialylation and sulfation, as well as residues such as N-acetylglucosamine, GalNAc, Mannose, and Galactose, were observed, suggesting a beneficial influence of chickpeas on mucosal integrity. In HFD + MG_13 these effects were reduced and resulted similar to the control. Conclusions: HFD reduces Muc2 expression in the colon and alters mucin composition: chickpea accessions, particularly MG_13, partially restore Muc2 levels and mucin oligosaccharide profiles, suggesting protective effects on the intestinal mucosal barrier.<\/jats:p>","DOI":"10.3390\/nu17193035","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:48:37Z","timestamp":1758635317000},"page":"3035","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mucin Alterations in Response to High-Fat Diet and the Potential Protective Role of Chickpea Accessions"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0680-5251","authenticated-orcid":false,"given":"Donatella","family":"Mentino","sequence":"first","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4447-3910","authenticated-orcid":false,"given":"Daniela","family":"Semeraro","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nastasia","family":"Taldone","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3666-7291","authenticated-orcid":false,"given":"Stefano","family":"Pavan","sequence":"additional","affiliation":[{"name":"Department of Soil, Plant, and Food Sciences, University of Bari Aldo Moro, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0740-7677","authenticated-orcid":false,"given":"Francesco","family":"Caponio","sequence":"additional","affiliation":[{"name":"Department of Soil, Plant, and Food Sciences, University of Bari Aldo Moro, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9426-2874","authenticated-orcid":false,"given":"Patrizia","family":"Gena","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3118-5813","authenticated-orcid":false,"given":"Marianna","family":"Ranieri","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8890-0278","authenticated-orcid":false,"given":"Grazia","family":"Tamma","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1296-861X","authenticated-orcid":false,"given":"Marco Vito","family":"Guglielmi","sequence":"additional","affiliation":[{"name":"National Research Council of Italy (CNR)\u2014Water Research Institute (IRSA), 74123 Taranto, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5888-7906","authenticated-orcid":false,"given":"Giovanni","family":"Scillitani","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy"},{"name":"National Research Council of Italy (CNR)\u2014Water Research Institute (IRSA), 74123 Taranto, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5308-2586","authenticated-orcid":false,"given":"Stefania","family":"Fensore","sequence":"additional","affiliation":[{"name":"Department of Socio-Economic, Managerial and Statistical Studies, University of Chieti-Pescara, 65129 Pescara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0799-8032","authenticated-orcid":false,"given":"Maria","family":"Mastrodonato","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy"},{"name":"National Research Council of Italy (CNR)\u2014Water Research Institute (IRSA), 74123 Taranto, 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