{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:27:29Z","timestamp":1789050449510,"version":"build-2803163510"},"reference-count":36,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Youth Research Project of Wuxi Municipal Health Commission","award":["Q202416"],"award-info":[{"award-number":["Q202416"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>[Introduction] The study aimed to investigate the protective effect of Lycium ruthenicum polysaccharides (LRPs) against carbon tetrachloride (CCl4)-induced acute liver injury (ALI). [Method] After LRP was extracted and characterized, a CCl4-induced cell damage and mouse ALI model was established to evaluate its anti-inflammatory and antioxidant capacities. [Results] The results demonstrated that LRP markedly attenuated hepatocyte necrosis, alleviated cellular edema and degeneration, and preserved nuclear integrity to sustain hepatic function, thereby restoring hepatic architecture. It downregulated serum ALT and AST levels, reduced MDA content in liver tissue, and enhanced SOD activity. Additionally, LRP inhibited the expression of pro-inflammatory cytokines TNF-\u03b1 and IL-6, while upregulating the anti-inflammatory cytokine IL-10. [Conclusions] These findings suggest that LRP effectively alleviates CCl4-induced ALI through both antioxidant and anti-inflammatory effects, demonstrating its potential as a novel liver-protective agent.<\/jats:p>","DOI":"10.3390\/nu17213359","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T07:31:58Z","timestamp":1761550318000},"page":"3359","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Lycium ruthenicum Polysaccharides Alleviate CCl4-Induced Acute Liver Injury Through Antioxidant and Anti-Inflammatory Effects"],"prefix":"10.3390","volume":"17","author":[{"given":"Jie","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Recreation and Sports and Health Tourism, Russian Sports University, Moscow 105122, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunpeng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Recreation and Sports and Health Tourism, Russian Sports University, Moscow 105122, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Pei","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhua","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haotian","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4390-8046","authenticated-orcid":false,"given":"Wen","family":"Xiang","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Wei","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1038\/s41401-019-0213-0","article-title":"Protective effects of specific cannabinoid receptor 2 agonist GW405833 on concanavalin A-induced acute liver injury in mice","volume":"40","author":"Huang","year":"2019","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mu, W., Wang, Q., Jia, M., Dong, S., Li, S., Yang, J., and Liu, G. (2022). Hepatoprotective Effects of Albumin-Encapsulated Nanoparticles of a Curcumin Derivative COP-22 against Lipopolysaccharide\/D-Galactosamine-Induced Acute Liver Injury in Mice. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23094903"},{"key":"ref_3","first-page":"251","article-title":"In vitro differentiation of rhesus macaque bone marrow- and adipose tissue-derived MSCs into hepatocyte-like cells","volume":"20","author":"Wang","year":"2020","journal-title":"Exp. Ther. Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"You, H., Wang, L., Bu, F., Meng, H., Huang, C., Fang, G., and Li, J. (2022). Ferroptosis: Shedding Light on Mechanisms and Therapeutic Opportunities in Liver Diseases. Cells, 11.","DOI":"10.3390\/cells11203301"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhao, J.H., Li, J., Zhang, X.Y., Shi, S., Wang, L., Yuan, M.L., Liu, Y.P., and Wang, Y.D. (2023). Confusoside from Anneslea fragrans Alleviates Acetaminophen-Induced Liver Injury in HepG2 via PI3K-CASP3 Signaling Pathway. Molecules, 28.","DOI":"10.3390\/molecules28041932"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Rashid, U., Khan, M.R., and Sajid, M. (2016). Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat. BMC Complement. Altern. Med., 16.","DOI":"10.1186\/s12906-016-1445-x"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Kinaneh, S., Hijaze, W., Mansour-Wattad, L., Hammoud, R., Zaidani, H., Kabala, A., and Hamoud, S. (2022). Heparanase Inhibition Prevents Liver Steatosis in E(0) Mice. J. Clin. Med., 11.","DOI":"10.3390\/jcm11061672"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wang, M., Niu, J., Ou, L., Deng, B., Wang, Y., and Li, S. (2019). Zerumbone Protects against Carbon Tetrachloride (CCl(4))-Induced Acute Liver Injury in Mice via Inhibiting Oxidative Stress and the Inflammatory Response: Involving the TLR4\/NF-\u03baB\/COX-2 Pathway. Molecules, 24.","DOI":"10.3390\/molecules24101964"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Yang, C., Wu, P., Cao, Y., Yang, B., Liu, L., Chen, J., Zhuo, R., and Yao, X. (2022). Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1038467"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8105306","DOI":"10.1155\/2022\/8105306","article-title":"Huangjia Ruangan Granule Inhibits Inflammation in a Rat Model with Liver Fibrosis by Regulating TNF\/MAPK and NF-\u03baB Signaling Pathways","volume":"2022","author":"Cai","year":"2022","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2953502","DOI":"10.1155\/2020\/2953502","article-title":"Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice","volume":"2020","author":"Xiao","year":"2020","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Cui, B., Liu, L., Shi, T., Yin, M., Feng, X., and Shan, Y. (2023). The Ethanolic Extract of Lycium ruthenicum Ameliorates Age-Related Physiological Damage in Mice. Molecules, 28.","DOI":"10.3390\/molecules28227615"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Dong, M., Lu, J., Xue, H., Lou, Y., Li, S., Liu, T., Ding, Z., and Chen, X. (2024). Anthocyanins from Lycium ruthenicum Murray Mitigate Cadmium-Induced Oxidative Stress and Testicular Toxicity by Activating the Keap1\/Nrf2 Signaling Pathway. Pharmaceuticals, 17.","DOI":"10.3390\/ph17030322"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/j.toxrep.2021.03.019","article-title":"Lycium barbarum polysaccharides inhibit ischemia\/reperfusion-induced myocardial injury via the Nrf2 antioxidant pathway","volume":"8","author":"Liu","year":"2021","journal-title":"Toxicol. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.ijbiomac.2014.03.023","article-title":"Lycium ruthenicum polysaccharide attenuates inflammation through inhibiting TLR4\/NF-\u03baB signaling pathway","volume":"67","author":"Peng","year":"2014","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2588","DOI":"10.1002\/fsn3.1445","article-title":"Lycium ruthenicum Murr. alleviates nonalcoholic fatty liver in mice","volume":"8","author":"Lu","year":"2020","journal-title":"Food Sci. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2956","DOI":"10.1002\/fsn3.2892","article-title":"Synergistic antitumor effects of polysaccharides and anthocyanins from Lycium ruthenicum Murr. on human colorectal carcinoma LoVo cells and the molecular mechanism","volume":"10","author":"Qin","year":"2022","journal-title":"Food Sci. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1076029620960820","DOI":"10.1177\/1076029620960820","article-title":"Comparative Studies on the Anticoagulant Profile of Branded Enoxaparin and a New Biosimilar Version","volume":"26","author":"Qneibi","year":"2020","journal-title":"Clin. Appl. Thromb. Hemost."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Song, Z., Zhang, Y., Luo, Y., Ti, Y., Wang, W., Ban, Y., Tang, Y., Hou, Y., Xu, L., and Ming, J. (2022). Systematic evaluation on the physicochemical characteristics of a series polysaccharides extracted from different edible lilies by ultrasound and subcritical water. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.998942"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Liu, X., Chen, S., Liu, H., Xie, J., Hasan, K.M.F., Zeng, Q., Wei, S., and Luo, P. (2023). Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa). Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1078868"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Liu, L.L., Li, M.Y., Sun, Y.H., Yang, X.Y., Ma, M.X., Wang, H., and An, M.Z. (2022). A Facile Microwave Hydrothermal Method for Fabricating SnO(2)@C\/Graphene Composite With Enhanced Lithium Ion Storage Properties. Front. Chem., 10.","DOI":"10.3389\/fchem.2022.895749"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s13568-019-0785-6","article-title":"Synthesis of gold nanoparticles derived from mannosylerythritol lipid and evaluation of their bioactivities","volume":"9","author":"Bakur","year":"2019","journal-title":"AMB Express"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7907814","DOI":"10.1155\/2022\/7907814","article-title":"The Polysaccharides from the Aerial Parts of Bupleurum chinense DC Attenuate Epilepsy-Like Behavior through Oxidative Stress Signaling Pathways","volume":"2022","author":"Li","year":"2022","journal-title":"Oxidative Med. Cell Longev."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Yang, Y., Yin, X., Zhang, D., Zhang, B., Lu, J., and Wang, X. (2022). Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp. Molecules, 27.","DOI":"10.3390\/molecules27144385"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Li, X., Hou, H., Liu, H., Wang, H., Cai, L., An, M., Zhang, C., and Wu, Y. (2023). Identification of 3-Methoxyphenylacetic Acid as a Phytotoxin, Produced by Rhizoctonia solani AG-3 TB. Molecules, 28.","DOI":"10.3390\/molecules28020790"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.carbpol.2008.12.015","article-title":"Chemical structural and chain conformational characterization of some bioactive polysaccharides isolated from natural sources","volume":"76","author":"Yang","year":"2009","journal-title":"Carbohydr. Polym."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Sassi Aydi, S., Aydi, S., Ben Khadher, T., Ktari, N., Merah, O., and Bouajila, J. (2023). Polysaccharides from South Tunisian Moringa alterniflora Leaves: Characterization, Cytotoxicity, Antioxidant Activity, and Laser Burn Wound Healing in Rats. Plants, 12.","DOI":"10.3390\/plants12020229"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Hu, M., Liu, Y., Wang, L., Wang, J., Li, L., and Wu, C. (2019). Purification, Characterization of Two Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine and Their Anti-Inflammatory Effects on Mucus Secretion of Airway Epithelium. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20143553"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ahn, J.C., Mathiyalagan, R., Nahar, J., Ramadhania, Z.M., Kong, B.M., Lee, D.W., Choi, S.K., Lee, C.S., Boopathi, V., and Yang, D.U. (2022). Transcriptome expression profile of compound-K-enriched red ginseng extract (DDK-401) in Korean volunteers and its apoptotic properties. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.999192"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3276148","DOI":"10.1155\/2020\/3276148","article-title":"MitoQ Modulates Lipopolysaccharide-Induced Intestinal Barrier Dysfunction via Regulating Nrf2 Signaling","volume":"2020","author":"Zhang","year":"2020","journal-title":"Mediat. Inflamm."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Lu, N., Xu, S., Xiang, W., Mei, X., Hu, H., Tang, X., Gong, X., and Wang, X. (2025). Lycium ruthenicum Murr. Polysaccharide Attenuated Inflammatory Response and Intestinal Flora Dysbiosis in LPS-Induced Acute Lung Injury in Mice. Nutrients, 17.","DOI":"10.3390\/nu17182944"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.5114\/aoms.2020.94236","article-title":"Evaluation of the mechanism of cordyceps polysaccharide action on rat acute liver failure","volume":"16","author":"Gu","year":"2020","journal-title":"Arch. Med. Sci."},{"key":"ref_33","first-page":"20631","article-title":"Immuno-enhancement effects of Lycium ruthenicum Murr. polysaccharide on cyclophosphamide-induced immunosuppression in mice","volume":"8","author":"Gong","year":"2015","journal-title":"Int. J. Clin. Exp. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"112518","DOI":"10.1016\/j.mce.2025.112518","article-title":"The spleen-liver axis supports obesity-induced systemic and fatty liver inflammation via MDSC and NKT cell enrichment","volume":"601","author":"Brummer","year":"2025","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"145969","DOI":"10.1016\/j.foodchem.2025.145969","article-title":"Structural characterization of \u03b2-glucans from five edible fungi and structure-activity relationship on Dectin-1\/Toll like receptors activation","volume":"493","author":"Liu","year":"2025","journal-title":"Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"123477","DOI":"10.1016\/j.carbpol.2025.123477","article-title":"Synthesis and biological evaluation of mirror isomers of \u03b2-(1\u21923)-glucans as immune modulators","volume":"357","author":"Sun","year":"2025","journal-title":"Carbohydr. Polym."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/21\/3359\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T07:36:30Z","timestamp":1761550590000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/21\/3359"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,25]]},"references-count":36,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["nu17213359"],"URL":"https:\/\/doi.org\/10.3390\/nu17213359","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,25]]}}}