{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T23:19:17Z","timestamp":1788477557948,"version":"build-2803163510"},"reference-count":116,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"C J Koh Endowment fund","award":["C220331005"],"award-info":[{"award-number":["C220331005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Intestinal health is essential for maintaining systemic physiological balance through nutrient absorption, immune regulation, and host\u2013microbiota interactions. Atractylodes macrocephala (Baizhu), a traditional medicinal plant long used for gastrointestinal dysfunction, has attracted growing interest because its polysaccharides (AMPs) show promises in intestinal disorders. In this review, we summarize preclinical studies on AMPs identified through searches of PubMed, Web of Science, ScienceDirect, Google Scholar and the China National Knowledge Infrastructure (CNKI), focusing on their extraction, purification, structural features and gut-related activities. Experimental evidence suggests that AMPs are metabolized by gut microbiota into short-chain fatty acids and other bioactive metabolites that regulate mucosal immunity, enhance epithelial barrier function and modulate host metabolic pathways. AMPs have been shown to promote the growth of beneficial taxa, restore dysbiotic communities, up-regulate tight junction proteins, suppress intestinal inflammation and modulate gut\u2013brain axis signaling involved in intestinal motility and visceral sensitivity. These actions underlie their protective effects reported in models of irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), colorectal cancer, chemotherapy-induced mucosal injury, and metabolic-associated systemic inflammation. Overall, current data support AMPs as microbiota-modulating, immunomodulatory, epithelial-protective and neuro-regulatory agents with potential as functional food-derived interventions for intestinal health. In this review, we also highlight key limitations and priorities for future research on structure\u2013function relationships and clinical translation.<\/jats:p>","DOI":"10.3390\/nu17233722","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T16:31:52Z","timestamp":1764261112000},"page":"3722","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Polysaccharides from Atractylodes macrocephala: A Review of Mechanistic and Therapeutic Insights into Intestinal Disorders"],"prefix":"10.3390","volume":"17","author":[{"given":"Meng","family":"Li","sequence":"first","affiliation":[{"name":"School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6510-421X","authenticated-orcid":false,"given":"Chester Yan Jie","family":"Ng","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huangyan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9137-1445","authenticated-orcid":false,"given":"Wai Ching","family":"Lam","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore"},{"name":"Department of Epidemiology, Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3877-1914","authenticated-orcid":false,"given":"Linda","family":"Zhong","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1097\/CM9.0000000000003615","article-title":"The signature of the small intestinal epithelial and immune cells in health and diseases","volume":"138","author":"Gao","year":"2025","journal-title":"Chin. Med. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1126\/science.1124234","article-title":"Metagenomic analysis of the human distal gut microbiome","volume":"312","author":"Gill","year":"2006","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1136\/gutjnl-2013-304739","article-title":"Colonic mucosa-associated diffusely adherent afaC+ Escherichia coli expressing lpfA and pks are increased in inflammatory bowel disease and colon cancer","volume":"63","author":"Friswell","year":"2014","journal-title":"Gut"},{"key":"ref_4","first-page":"560","article-title":"Gut bacteria in health and disease","volume":"9","author":"Quigley","year":"2013","journal-title":"Gastroenterol. Hepatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/s41575-020-0286-8","article-title":"Global burden of irritable bowel syndrome: Trends, predictions and risk factors","volume":"17","author":"Black","year":"2020","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s11739-023-03374-w","article-title":"Gut microbiota, intestinal permeability, and systemic inflammation: A narrative review","volume":"19","author":"Petito","year":"2024","journal-title":"Intern. Emerg. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2331460","DOI":"10.1080\/19490976.2024.2331460","article-title":"Breaking the barriers: The role of gut homeostasis in Metabolic-Associated Steatotic Liver Disease (MASLD)","volume":"16","author":"Cubero","year":"2024","journal-title":"Gut Microbes"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"9621668","DOI":"10.1155\/2022\/9621668","article-title":"Inflammatory bowel disease therapeutics: A focus on probiotic engineering","volume":"2022","author":"Mishra","year":"2022","journal-title":"Mediat. Inflamm."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Dahiya, D., and Nigam, P.S. (2023). Biotherapy using probiotics as therapeutic agents to restore the gut microbiota to relieve gastrointestinal tract inflammation, IBD, IBS and prevent induction of cancer. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065748"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jep.2018.08.023","article-title":"The traditional uses, phytochemistry, and pharmacology of Atractylodes macrocephala Koidz.: A review","volume":"226","author":"Zhu","year":"2018","journal-title":"J. Ethnopharmacol."},{"key":"ref_11","first-page":"9420089","article-title":"Pharmacological Properties of Atractylodes macrocephala Koidz.: A Comprehensive Review","volume":"3","author":"Wang","year":"2025","journal-title":"Food Med. Homol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"12999","DOI":"10.1080\/10408398.2023.2259461","article-title":"Recent advances in polysaccharide biomodification by microbial fermentation: Production, properties, bioactivities, and mechanisms","volume":"64","author":"Wang","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1080\/10408398.2022.2119931","article-title":"Insoluble dietary fibers: Structure, metabolism, interactions with human microbiome, and role in gut homeostasis","volume":"64","author":"Baky","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.carbpol.2015.05.079","article-title":"Structure-function relationships of immunostimulatory polysaccharides: A review","volume":"132","author":"Ferreira","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Zhao, L., Zhang, T., and Zhang, K. (2024). Pharmacological effects of ginseng and ginsenosides on intestinal inflammation and the immune system. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1353614"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Liang, H., Tao, S., Wang, Y., Zhao, J., Yan, C., Wu, Y., Liu, N., and Qin, Y. (2024). Astragalus polysaccharide: Implication for intestinal barrier, anti-inflammation, and animal production. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1364739"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4125","DOI":"10.1002\/ptr.7607","article-title":"Crosstalk between traditional Chinese medicine-derived polysaccharides and the gut microbiota: A new perspective to understand traditional Chinese medicine","volume":"36","author":"Yue","year":"2022","journal-title":"Phytother. Res."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zhao, T., Wang, C., Liu, Y., Li, B., Shao, M., Zhao, W., and Zhou, C. (2025). The role of polysaccharides in immune regulation through gut microbiota: Mechanisms and implications. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1555414"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Kong, C., Zhou, S., Xu, C., Yang, J., Chen, J., Li, C., Yuan, Y., Peng, Z., Chang, G., and Yang, Y. (2025). Polysaccharides of Atractylodes macrocephala Koidz effectively improve the EpCAM(+\/\u2212) genotype-related inflammatory bowel diseases via modulating gut microbiota. Int. J. Biol. Macromol., 333.","DOI":"10.1016\/j.ijbiomac.2025.148855"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Luo, W., Zhang, K., Wang, Y., Ye, M., Zhang, Y., Xu, W., Chen, L., and Li, H. (2025). The Rhizome of Atractylodes macrocephala Koidz.: A comprehensive review on the traditional Uses, phytochemistry and pharmacology. Chem. Biodivers., 22.","DOI":"10.1002\/cbdv.202401879"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Jeong, D., Dong, G.Z., Lee, H.J., and Ryu, J.H. (2019). Anti-inflammatory compounds from Atractylodes macrocephala. Molecules, 24.","DOI":"10.3390\/molecules24101859"},{"key":"ref_22","first-page":"206","article-title":"Advances in the study of Atractylodes macrocephala Koidz","volume":"25","author":"Yang","year":"2002","journal-title":"Zhong Yao Cai"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Lin, F., Xu, Y., Liu, B., Li, H., and Chen, L. (2024). Research progress on extraction, separation, structure, and biological activities of polysaccharides from the genus Atractylodes: A review. Int. J. Biol. Macromol., 283.","DOI":"10.1016\/j.ijbiomac.2024.137550"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"115506","DOI":"10.1016\/j.jep.2022.115506","article-title":"Isolation, structure and bioactivity of polysaccharides from Atractylodes macrocephala: A review","volume":"296","author":"Li","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1016\/j.carbpol.2017.07.041","article-title":"Structural analysis and immunomodulatory effect of polysaccharide from Atractylodis macrocephalae Koidz. on bovine lymphocytes","volume":"174","author":"Xu","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.ijbiomac.2020.06.269","article-title":"Isolation, purification, and structural characterization of polysaccharides from Atractylodis macrocephalae Rhizoma and their immunostimulatory activity in RAW264.7 cells","volume":"163","author":"Cui","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.ijbiomac.2020.11.144","article-title":"Atractylodis macrocephalae polysaccharides protect against DSS-induced intestinal injury through a novel lncRNA ITSN1-OT1","volume":"167","author":"Zong","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"926381","DOI":"10.1155\/2014\/926381","article-title":"The metabolism of polysaccharide from Atractylodes macrocephala Koidz and its effect on intestinal microflora","volume":"2014","author":"Wang","year":"2014","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Liu, X., Lin, X., Hong, H., Wang, J., Tao, Y., Huai, Y., Pang, H., Liu, M., Li, J., and Bo, R. (2024). Polysaccharide from Atractylodes macrocephala Koidz binding with Zinc Oxide Nanoparticles as a novel mucosal immune adjuvant for H9N2 inactivated vaccine. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25042132"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2338533","DOI":"10.1155\/2022\/2338533","article-title":"Polysaccharides from Rhizoma Atractylodis macrocephalae: A review on their extraction, purification, structure, and bioactivities","volume":"2022","author":"Luo","year":"2022","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ji, X., Yin, M., Nie, H., and Liu, Y. (2020). A review of isolation, chemical properties, and bioactivities of polysaccharides from Bletilla striata. Biomed. Res. Int., 2020.","DOI":"10.1155\/2020\/5391379"},{"key":"ref_32","first-page":"77","article-title":"Study on the extraction technology of alkali-soluble polysaccharides from Atractylodis macrocephalae Koidz","volume":"1","author":"He","year":"2010","journal-title":"Med. Plant"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"22220","DOI":"10.3390\/molecules201219837","article-title":"Multi-optimization of ultrasonic-assisted enzymatic extraction of Atratylodes macrocephala polysaccharides and antioxidants using response surface methodology and desirability function approach","volume":"20","author":"Pu","year":"2015","journal-title":"Molecules"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"18375","DOI":"10.1021\/acsomega.4c00364","article-title":"Ultrasound-Assisted Extraction of Atractylodes chinensis (DC.) Koidz. Polysaccharides and the Synergistic Antigastric Cancer Effect in Combination with Oxaliplatin","volume":"9","author":"Liang","year":"2024","journal-title":"ACS Omega"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2200114","DOI":"10.1002\/star.202200114","article-title":"Extraction, separation, purification, chemical characterizations, and biological activities of polysaccharides from Chinese herbal medicine: A review","volume":"74","author":"Zhang","year":"2022","journal-title":"Starch-St\u00e4rke"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Zhou, P., Xiao, W., Wang, X., Wu, Y., Zhao, R., and Wang, Y. (2022). A comparison study on polysaccharides extracted from Atractylodes chinensis (DC.) Koidz. Using different methods: Structural characterization and anti-SGC-7901 effect of combination with Apatinib. Molecules, 27.","DOI":"10.3390\/molecules27154727"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Yang, R., Fan, H., He, B., Ruan, Q., Wei, B., Han, B., Hao, X., Maoz, I., and Kai, G. (2023). Current progress of Atractylodes macrocephala Koidz.: A review of its biogeography, PAO-ZHI processing, biological activities, biosynthesis pathways, and technology applications. Med. Plant Biol., 2.","DOI":"10.48130\/MPB-2023-0005"},{"key":"ref_38","first-page":"647","article-title":"Studies on separation, purification and chemical structure of polysaccharide from Atractylodes macrocephala","volume":"24","author":"Chi","year":"2001","journal-title":"Zhong Yao Cai"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1007\/s10142-022-00863-7","article-title":"Effects of PAMK on lncRNA, miRNA, and mRNA expression profiles of thymic epithelial cells","volume":"22","author":"Wu","year":"2022","journal-title":"Funct. Integr. Genom."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.carbpol.2015.03.034","article-title":"Immune-enhancing activity of polysaccharides isolated from Atractylodis macrocephalae Koidz","volume":"126","author":"Sun","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"100224","DOI":"10.1016\/j.jcoa.2025.100224","article-title":"Determination of the composition and monosaccharide content of Atractylodes Polysaccharides using pre-column derivatization and a quantitative analysis of multicomponents by a single marker method","volume":"7","author":"Tu","year":"2025","journal-title":"J. Chromatogr. Open"},{"key":"ref_42","first-page":"p631","article-title":"Chromatographic analysis and atomic force microscope observation of polysaccharide extracted from Atractylodes macrocephala Koidz","volume":"24","author":"Wu","year":"2012","journal-title":"Nat. Prod. Res. Dev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.carbpol.2016.07.116","article-title":"Structure characterization of three polysaccharides and a comparative study of their immunomodulatory activities on chicken macrophage","volume":"153","author":"Fan","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"109777","DOI":"10.1016\/j.foodres.2020.109777","article-title":"Polysaccharides from Atractylodes macrocephala Koidz. Ameliorate ulcerative colitis via extensive modification of gut microbiota and host metabolism","volume":"138","author":"Feng","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"107692","DOI":"10.1016\/j.intimp.2021.107692","article-title":"Polysaccharide of Atractylodes macrocephala Koidz regulates LPS-mediated mouse hepatitis through the TLR4-MyD88-NFkappaB signaling pathway","volume":"98","author":"Guo","year":"2021","journal-title":"Int. Immunopharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.ijbiomac.2016.01.086","article-title":"Protective effect of a polysaccharide from Rhizoma Atractylodis macrocephalae on acute liver injury in mice","volume":"87","author":"Han","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"512","DOI":"10.3109\/13880209.2014.929152","article-title":"Macrophage activation by polysaccharides from Atractylodes macrocephala Koidz through the nuclear factor-kappaB pathway","volume":"53","author":"Ji","year":"2015","journal-title":"Pharm. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Li, W., Wang, X., Sun, X., Zhang, Y., and Xie, J. (2025). Stir-frying duration modulates structural characterization and functional properties of Atractylodes macrocephala polysaccharides. Int. J. Biol. Macromol., 306.","DOI":"10.1016\/j.ijbiomac.2025.141489"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Liang, Z., Yuan, Y., Liang, J., Wu, Y., Cui, J., Gu, H., Pi, D., Yi, Z., and Zhou, S. (2025). Atractylodes macrocephala Koidz. Polysaccharide Alleviates Chemotherapy-Induced Depression-Like Behaviors Through the Gut-Brain Axis. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262010189"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1080\/14786419.2014.995652","article-title":"Characterisation of oligosaccharides from Baizhu by HILIC-MS","volume":"29","author":"Lin","year":"2015","journal-title":"Nat. Prod. Res."},{"key":"ref_51","first-page":"438","article-title":"Studies on physico-chemical properties and hypoglycemic activity of complex polysaccharide AMP-B from Atractylodes macrocephala Koidz","volume":"38","author":"Shan","year":"2003","journal-title":"Yao Xue Xue Bao"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Tang, X., Liu, L., Wu, Y., Zhao, Y., Lu, C., and Zhao, R. (2024). An inulin-type polysaccharide from Atractylodis macrocephalae Rhizoma can relieve psoriasis. Int. J. Biol. Macromol., 280.","DOI":"10.1016\/j.ijbiomac.2024.135667"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.aej.2025.02.099","article-title":"Preparation and potential drug delivery applications of Atractylodes macrocephala polysaccharide-based nanoparticles","volume":"121","author":"Yue","year":"2025","journal-title":"Alex. Eng. J."},{"key":"ref_54","first-page":"674","article-title":"Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K(+) channel","volume":"16","author":"Zeng","year":"2018","journal-title":"Chin. J. Nat. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"101397","DOI":"10.1016\/j.rechem.2024.101397","article-title":"Structural characterization, immunomodulatory effect and immune-mediated antitumor activity of a novel polysaccharide from the rhizome of Atractylodis macrocephala Koidz","volume":"7","author":"Zhuang","year":"2024","journal-title":"Results Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"41","DOI":"10.2174\/0115734110283469231204061131","article-title":"Quantitative HPLC-based metabolomics approach for the discrimination of processed rhizomes of Atractylodes macrocephala","volume":"20","author":"Hai","year":"2024","journal-title":"Curr. Anal. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"155739","DOI":"10.1016\/j.phymed.2024.155739","article-title":"*The traditional Chinese medicine processing change chemical composition and pharmacological effectiveness: Taking Atractylodes macrocephala Koidz. and honey bran-fried Atractylodes macrocephala Koidz. as examples","volume":"130","author":"Hao","year":"2024","journal-title":"Phytomedicine"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"100850","DOI":"10.1016\/j.cofs.2022.100850","article-title":"Food glycomics in food science: Recent advances and future perspectives","volume":"46","author":"Tang","year":"2022","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.carbpol.2017.09.074","article-title":"Quantification of food polysaccharide mixtures by (1)H NMR","volume":"179","author":"Merkx","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Zeng, W., Chen, L., Xiao, Z., Li, Y., Ma, J., Ding, J., and Yang, J. (2023). Comparative study on the structural properties and bioactivities of three different molecular weights of lycium barbarum Polysaccharides. Molecules, 28.","DOI":"10.3390\/molecules28020701"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.ijbiomac.2020.06.003","article-title":"Immunomodulatory activity-guided isolation and characterization of a novel polysaccharide from Atractylodis macrocephalae Koidz","volume":"161","author":"Xue","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Bo, R., Liu, X., Wang, J., Wei, S., Wu, X., Tao, Y., Xu, S., Liu, M., Li, J., and Pang, H. (2022). Polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles: Characterization, immunological effect and mechanism. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.992502"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Yuan, S., Li, Y., Li, J., Xue, J.C., Wang, Q., Hou, X.T., Meng, H., Nan, J.X., and Zhang, Q.G. (2022). Traditional Chinese Medicine and Natural Products: Potential Approaches for Inflammatory Bowel Disease. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.892790"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"12242","DOI":"10.1080\/10408398.2023.2248631","article-title":"Recent progress in plant-derived polysaccharides with prebiotic potential for intestinal health by targeting gut microbiota: A review","volume":"64","author":"Wang","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Huang, S., He, H., Li, H., Li, C., Wang, F., Hu, Y., Liu, Y., Chen, L., and Chen, H. (2025). Modulating the intestinal flora involves the effect of Atractylodis macrocephalae Rhizoma polysaccharide on spleen deficiency diarrhea. Int. J. Biol. Macromol., 321.","DOI":"10.1016\/j.ijbiomac.2025.146562"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Hu, P., Yan, X., Zeng, Y., Jiang, Z., Liu, J., and Feng, W.W. (2023). An UPLC-MS\/MS method for targeted analysis of microbial and host tryptophan metabolism after administration of polysaccharides from Atractylodes macrocephala Koidz. in ulcerative colitis mice. J. Pharm. Biomed. Anal., 235.","DOI":"10.1016\/j.jpba.2023.115585"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"105160","DOI":"10.1016\/j.psj.2025.105160","article-title":"Protective effects of polysaccharide of Atractylodes macrocephala Koidz and Jiawei Si-jun-zi Decoction on gut health and immune function in cyclophosphamide-treated chicks","volume":"104","author":"Lu","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.ijbiomac.2021.12.042","article-title":"Protective effects of polysaccharides from Atractylodes macrocephalae Koidz. against dextran sulfate sodium induced intestinal mucosal injury on mice","volume":"195","author":"Kai","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Li, W., Lu, B., Pan, S., Bai, S., He, J., Li, B., Cao, N., Fu, X., Wei, J., and Chen, Y. (2025). Protection of LPS-induced intestinal injury in goslings by polysaccharide of Atractylodes macrocephala Koidz based on 16S rRNA and metabolomics analysis. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0326164"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"e14875","DOI":"10.1111\/nmo.14875","article-title":"Polysaccharide extracted from Atractylodes macrocephala improves the spleen deficiency constipation in mice by regulating the gut microbiota to affect the 5-HT synthesis","volume":"36","author":"Chen","year":"2024","journal-title":"Neurogastroenterol. Motil."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.immuni.2018.12.018","article-title":"The Short Chain Fatty Acid Butyrate Imprints an Antimicrobial Program in Macrophages","volume":"50","author":"Schulthess","year":"2019","journal-title":"Immunity"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1186\/s12964-023-01219-9","article-title":"Short-chain fatty acids in diseases","volume":"21","author":"Zhang","year":"2023","journal-title":"Cell Commun. Signal."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"He, Z., Guo, J., Zhang, H., Yu, J., Zhou, Y., Wang, Y., Li, T., Yan, M., Li, B., and Chen, Y. (2023). Atractylodes macrocephala Koidz polysaccharide improves glycolipid metabolism disorders through activation of aryl hydrocarbon receptor by gut flora-produced tryptophan metabolites. Int. J. Biol. Macromol., 253.","DOI":"10.1016\/j.ijbiomac.2023.126987"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1111\/bph.17409","article-title":"Atractylodes macrocephala Koidz polysaccharide ameliorates DSS-induced colitis in mice by regulating the gut microbiota and tryptophan metabolism","volume":"182","author":"Zhang","year":"2025","journal-title":"Br. J. Pharmacol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"102285","DOI":"10.1016\/j.psj.2022.102285","article-title":"Polysaccharide of Atractylodes macrocephala Koidz alleviate lipopolysaccharide-stimulated liver inflammation injury of goslings through miR-223\/NLRP3 axis","volume":"102","author":"Chen","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"119884","DOI":"10.1016\/j.jep.2025.119884","article-title":"Rhizome of Atractylodes macrocephala alleviates spleen-deficiency constipation in rats by modulating gut microbiota and bile acid metabolism","volume":"348","author":"Yang","year":"2025","journal-title":"J. Ethnopharmacol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/S2468-1253(24)00390-X","article-title":"The intestinal barrier: A pivotal role in health, inflammation, and cancer","volume":"10","author":"Neurath","year":"2025","journal-title":"Lancet Gastroenterol. Hepatol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-018-0126-x","article-title":"Mechanisms regulating intestinal barrier integrity and its pathological implications","volume":"50","author":"Chelakkot","year":"2018","journal-title":"Exp. Mol. Med."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/nri2653","article-title":"Intestinal mucosal barrier function in health and disease","volume":"9","author":"Turner","year":"2009","journal-title":"Nat. Rev. Immunol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1136\/gutjnl-2019-318427","article-title":"Leaky gut: Mechanisms, measurement and clinical implications in humans","volume":"68","author":"Camilleri","year":"2019","journal-title":"Gut"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"156977","DOI":"10.1016\/j.phymed.2025.156977","article-title":"Study on the effects of the Atractylodes macrocephala koidz-raphanus sativus l herb pair in alleviating senile constipation via the gut microbiota-SCFAs-5-HT axis","volume":"145","author":"Gao","year":"2025","journal-title":"Phytomedicine"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Ran, P., Jiang, F., Pan, L., Shu, Y., Hu, F., Wang, Y., Zhao, R., Wang, W., Mu, H., and Wang, J. (2025). Polysaccharide from Atractylodes macrocephala Koidz. alleviates pyrotinib-induced diarrhea through regulating cAMP\/LKB1\/AMPK\/CFTR pathway and restoring gut microbiota and metabolites. Int. J. Biol. Macromol., 308.","DOI":"10.1016\/j.ijbiomac.2025.142512"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1038\/ni.3319","article-title":"An innate antiviral pathway acting before interferons at epithelial surfaces","volume":"17","author":"Iversen","year":"2016","journal-title":"Nat. Immunol."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Gong, S., Zhang, B., Sun, X., Liang, W., Hong, L., Zhou, X., Li, W., Tian, Y., Xu, D., and Wu, Z. (2025). Polysaccharides of Atractylodes macrocephala Koidz alleviate LPS-induced bursa of fabricius injury in goslings by inhibiting EREG expression. Animals, 15.","DOI":"10.3390\/ani15010084"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Liu, C., Wang, S., Xiang, Z., Xu, T., He, M., Xue, Q., Song, H., Gao, P., and Cong, Z. (2022). The chemistry and efficacy benefits of polysaccharides from Atractylodes macrocephala Koidz. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.952061"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1089\/jmf.2017.3943","article-title":"Glycoproteins and Polysaccharides are the Main Class of Active Constituents Required for Lymphocyte Stimulation and Antigen-Specific Immune Response Induction by Traditional Medicinal Herbal Plants","volume":"20","author":"Son","year":"2017","journal-title":"J. Med. Food"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1016\/j.ijbiomac.2014.10.022","article-title":"Effect of selenylation modification on immune-enhancing activity of Atractylodes macrocephala polysaccharide","volume":"72","author":"Liu","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1089\/jmf.2018.4393","article-title":"Polysaccharide of Atractylodes macrocephala Koidz Enhances Cytokine Secretion by Stimulating the TLR4-MyD88-NF-kappaB Signaling Pathway in the Mouse Spleen","volume":"22","author":"Li","year":"2019","journal-title":"J. Med. Food."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Li, W., Guo, S., Xu, D., Li, B., Cao, N., Tian, Y., and Jiang, Q. (2018). Polysaccharide of Atractylodes macrocephala Koidz (PAMK) Relieves Immunosuppression in Cyclophosphamide-Treated Geese by Maintaining a Humoral and Cellular Immune Balance. Molecules, 23.","DOI":"10.3390\/molecules23040932"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Yang, M., Zhang, Q., Taha, R., Abdelmotalab, M.I., Wen, Q., Yuan, Y., Zhao, Y., Li, Q., Liao, C., and Huang, X. (2022). Polysaccharide from Atractylodes macrocephala Koidz. ameliorates DSS-induced colitis in mice by regulating the Th17\/Treg cell balance. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.1021695"},{"key":"ref_91","first-page":"9721353","article-title":"PAMK Relieves LPS-Induced Enteritis and Improves Intestinal Flora Disorder in Goslings","volume":"2021","author":"Li","year":"2021","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Chen, X., Yang, S., Long, X., Li, W., Li, B., Fu, C., Zhen, C., Xu, D., Fu, X., and Cao, N. (2025). Polysaccharide of Atractylodes macrocephala Koidz alleviate LPS-induced inflammatory liver injury by reducing pyroptosis of macrophage via regulating LncRNA GAS5\/miR-223-3p\/NLRP3 axis. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1593689"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"120063","DOI":"10.1016\/j.jep.2025.120063","article-title":"Polysaccharide of Atractylodes macrocephala Koidz alleviated fructose-induced salt-sensitive hypertension and renal injury via suppressing SIRT1\/Nlrp3\/caspase-1 pathway in mice","volume":"350","author":"Deng","year":"2025","journal-title":"J. Ethnopharmacol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"106084","DOI":"10.1016\/j.micpath.2023.106084","article-title":"A. macrocephala polysaccharide induces alterations to gut microbiome and serum metabolome in constipated mice","volume":"178","author":"Yang","year":"2023","journal-title":"Microb. Pathog."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.carbpol.2012.07.083","article-title":"Atractylodis macrocephalae Koidz. polysaccharides enhance both serum IgG response and gut mucosal immunity","volume":"91","author":"Xie","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"7111","DOI":"10.2147\/OTT.S219623","article-title":"Atractylodes macrocephala polysaccharides regulate the innate immunity of colorectal cancer cells by modulating the TLR4 signaling pathway","volume":"12","author":"Feng","year":"2019","journal-title":"OncoTargets Ther."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Yang, J., Ou, W., Lin, G., Wang, Y., Chen, D., Zeng, Z., Chen, Z., Lu, X., Wu, A., and Lin, C. (2024). PAMK Ameliorates Non-Alcoholic Steatohepatitis and Associated Anxiety\/Depression-like Behaviors Through Restoring Gut Microbiota and Metabolites in Mice. Nutrients, 16.","DOI":"10.3390\/nu16223837"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"eaaf6397","DOI":"10.1126\/scitranslmed.aaf6397","article-title":"Transplantation of fecal microbiota from patients with irritable bowel syndrome alters gut function and behavior in recipient mice","volume":"9","author":"Lynch","year":"2017","journal-title":"Sci. Transl. Med."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"G529","DOI":"10.1152\/ajpgi.00207.2012","article-title":"Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome","volume":"305","author":"Ringel","year":"2013","journal-title":"Am. J. Physiol.-Gastrointest. Liver Physiol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/s41575-018-0034-5","article-title":"New treatments and therapeutic targets for IBS and other functional bowel disorders","volume":"15","author":"Simren","year":"2018","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"2652","DOI":"10.1056\/NEJMra2002697","article-title":"Pathophysiology of Inflammatory Bowel Diseases","volume":"383","author":"Chang","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1038\/s41590-019-0415-0","article-title":"Targeting immune cell circuits and trafficking in inflammatory bowel disease","volume":"20","author":"Neurath","year":"2019","journal-title":"Nat. Immunol."},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Mao, M., Meng, Q., Shentu, C., Dai, S., Zhu, N., Hu, C., Wu, Y., and Yuan, X. (2025). Atractylodes macrocephala polysaccharides 1 ameliorate ulcerative colitis by regulating gut microbiota and IL-17RA signaling pathway. Food Sci. Hum. Wellness.","DOI":"10.26599\/FSHW.2025.9250651"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1111\/joim.13171","article-title":"Aspects of colorectal cancer screening, methods, age and gender","volume":"289","author":"Hultcrantz","year":"2021","journal-title":"J. Intern. Med."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1002329","DOI":"10.37349\/etat.2025.1002329","article-title":"Dysbiosis and colorectal cancer: Conducive factors, biological and molecular role, and therapeutic prospectives","volume":"6","author":"Singh","year":"2025","journal-title":"Explor. Target Antitumor Ther."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Fan, J., Zhu, J., Zhu, H., Zhang, Y., and Xu, H. (2023). Potential therapeutic target for polysaccharide inhibition of colon cancer progression. Front. Med., 10.","DOI":"10.3389\/fmed.2023.1325491"},{"key":"ref_107","first-page":"1209","article-title":"Polysaccharide of Atractylodes macrocephala inhibits the growth of mice in-situ colon cancer HT-29 cell xenograft via activating immune cells","volume":"6","author":"Feng","year":"2019","journal-title":"Chin. J. Cancer Biother."},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Hong, B., Sobue, T., Choquette, L., Dupuy, A., Thompson, A., Burleson, J., Salner, A., Schauer, P., Joshi, P., and Fox, E. (2019). Chemotherapy-induced oral mucositis is associated with detrimental bacterial dysbiosis. Microbiome, 7.","DOI":"10.1186\/s40168-019-0679-5"},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"van Vliet, M.J., Harmsen, H.J., de Bont, E.S., and Tissing, W.J. (2010). The role of intestinal microbiota in the development and severity of chemotherapy-induced mucositis. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1000879"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"v139","DOI":"10.1093\/annonc\/mdv202","article-title":"Management of oral and gastrointestinal mucosal injury: ESMO Clinical Practice Guidelines for diagnosis, treatment, and follow-up","volume":"26","author":"Peterson","year":"2015","journal-title":"Ann. Oncol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/nature11552","article-title":"Functional interactions between the gut microbiota and host metabolism","volume":"489","author":"Tremaroli","year":"2012","journal-title":"Nature"},{"key":"ref_112","doi-asserted-by":"crossref","unstructured":"Woting, A., and Blaut, M. (2016). The Intestinal Microbiota in Metabolic Disease. Nutrients, 8.","DOI":"10.3390\/nu8040202"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1016\/j.jhep.2019.10.003","article-title":"The gut-liver axis in liver disease: Pathophysiological basis for therapy","volume":"72","author":"Albillos","year":"2020","journal-title":"J. Hepatol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"113014","DOI":"10.1016\/j.intimp.2024.113014","article-title":"Polysaccharide of Atractylodes macrocephala Koidz alleviates NAFLD-induced hepatic inflammation in mice by modulating the TLR4\/MyD88\/NF-kappaB pathway","volume":"141","author":"Chen","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1142\/S0192415X11008981","article-title":"Protective effects of Atractylodes macrocephala polysaccharide on liver ischemia-reperfusion injury and its possible mechanism in rats","volume":"39","author":"Jin","year":"2011","journal-title":"Am. J. Chin. Med."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"102480","DOI":"10.1016\/j.psj.2023.102480","article-title":"Polysaccharide of Atractylodes macrocephala Koidz alleviate lipopolysaccharide-induced liver injury in goslings via the p53 and FOXO pathways","volume":"102","author":"Zhang","year":"2023","journal-title":"Poult. Sci."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/23\/3722\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T05:32:01Z","timestamp":1764394321000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/17\/23\/3722"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,27]]},"references-count":116,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["nu17233722"],"URL":"https:\/\/doi.org\/10.3390\/nu17233722","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,27]]}}}