{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:09:21Z","timestamp":1770883761817,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T00:00:00Z","timestamp":1766188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Next Generation EU in the framework of PRIN 2022","award":["CUP I53D23004270006"],"award-info":[{"award-number":["CUP I53D23004270006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Alzheimer\u2019s disease and Parkinson\u2019s disease are multifactorial disorders causing severe disability, rising with the increase in life expectancy. Currently, the identification of natural compounds useful against these disorders is becoming an urgent necessity. In this study, we used polyphenol-enriched extracts obtained from leaves of Mediterranean plants, which are important in animal feeding (Lotus ornithopodioides, Hedysarum coronarium, Medicago sativa) and in the human Mediterranean diet (Cichorium intybus). Objectives: The aims of this study were as follows: (i) tentative identification of the organic compounds present in the extracts; (ii) determination of their effect on the activity of monoamine oxidase (MAO)-A and MAO-B, key enzymes involved in the metabolism of aminergic neurotransmitters, as well as on protein expression level of these enzymes in cell lines expressing basal MAO-A and MAO-B. Methods: The ability of plant polyphenol extracts to inhibit MAO-A and MAO-B activity was assessed by in vitro enzyme assays. The protein expression level was analyzed by Western blotting. Results: Our data demonstrate that all the extracts behaved as MAO-A and MAO-B inhibitors, although to a different extent and enzyme inhibition mechanism; among them, the extract from L. ornithopodioides induced a decrease in MAO-A protein level in human AGS gastric adenocarcinoma and SH-SY5Y neuroblastoma cell lines. Conclusions: These data reinforce the hypothesis that a plant-based diet and\/or integrative supplementation of pharmacological treatments can be considered for preventing and relieving symptoms of neurodegenerative diseases.<\/jats:p>","DOI":"10.3390\/nu18010022","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T08:35:27Z","timestamp":1766392527000},"page":"22","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Polyphenol-Enriched Extracts from Leaves of Mediterranean Plants as Natural Inhibitors of Monoamine Oxidase (MAO)-A and MAO-B Enzymes"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3783-2535","authenticated-orcid":false,"given":"Antonio","family":"D\u2019Errico","sequence":"first","affiliation":[{"name":"Department of Medical, Movement and Well-Being Sciences, University of Naples \u201cParthenope\u201d, Via Medina, 40, 80133 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6241-4856","authenticated-orcid":false,"given":"Rosarita","family":"Nasso","sequence":"additional","affiliation":[{"name":"Department of Medical, Movement and Well-Being Sciences, University of Naples \u201cParthenope\u201d, Via Medina, 40, 80133 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5359-6301","authenticated-orcid":false,"given":"Mario","family":"Ruggiero","sequence":"additional","affiliation":[{"name":"Department of Medical, Movement and Well-Being Sciences, University of Naples \u201cParthenope\u201d, Via Medina, 40, 80133 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9964-0862","authenticated-orcid":false,"given":"Rosario","family":"Rullo","sequence":"additional","affiliation":[{"name":"Institute for the Animal Production Systems in the Mediterranean Environment, Consiglio Nazionale Delle Ricerche, Piazzale Enrico Fermi 1, 80055 Portici, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6812-2747","authenticated-orcid":false,"given":"Emmanuele","family":"De Vendittis","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via S. Pansini 5, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4485-7383","authenticated-orcid":false,"given":"Mariorosario","family":"Masullo","sequence":"additional","affiliation":[{"name":"Department of Medical, Movement and Well-Being Sciences, University of Naples \u201cParthenope\u201d, Via Medina, 40, 80133 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5217-4710","authenticated-orcid":false,"given":"Filomena","family":"Mazzeo","sequence":"additional","affiliation":[{"name":"Department of Economics, Law, Cybersecurity and Sport Sciences, University of Naples \u201cParthenope\u201d, Via Della Repubblica, 32, 80035 Nola, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8507-4361","authenticated-orcid":false,"given":"Rosaria","family":"Arcone","sequence":"additional","affiliation":[{"name":"Department of Medical, Movement and Well-Being Sciences, University of Naples \u201cParthenope\u201d, Via Medina, 40, 80133 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S1474-4422(19)30411-9","article-title":"The global burden of neurological disorders: Translating evidence into policy","volume":"19","author":"Feigin","year":"2020","journal-title":"Lancet Neurol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/s41514-023-00120-6","article-title":"Assessing cognitive decline in the aging brain: Lessons from rodent and human studies","volume":"9","author":"Brito","year":"2023","journal-title":"npj Aging"},{"key":"ref_3","first-page":"S87","article-title":"Parkinson\u2019s disease: Health-related quality of life, economic cost, and implications of early treatment","volume":"16","author":"Chen","year":"2010","journal-title":"Am. 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