{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T18:01:13Z","timestamp":1769536873727,"version":"3.49.0"},"reference-count":57,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Malnutrition and sleep disturbances are common in childhood and are associated with neuropeptides that regulate appetite and circadian rhythms. Hypothalamic peptides such as orexin A, agouti-related protein (AgRP), proopiomelanocortin (POMC), and peptide YY (PYY) play important roles in energy balance and eating behavior; however, their specific functions in pediatric malnutrition remain unclear. This study aimed to determine the levels of these peptides in malnourished children and to examine their relationship with eating and sleep behaviors. Methods: This case\u2013control, cross-sectional study included 99 children aged 5\u201315 years diagnosed with malnutrition and 85 age-matched healthy controls. Blood samples were collected from all participants, and peptide levels were measured using ELISA. Additionally, the Children\u2019s Eating Behaviour Questionnaire (CEBQ) and the Children\u2019s Sleep Habits Questionnaire (CSHQ) were administered to assess eating patterns and sleep behaviors. Statistical comparisons and hierarchical logistic regression analyses were performed. Results: Orexin A and PYY levels were significantly higher in malnourished children than in controls (p &lt; 0.001). No significant group differences were found for AgRP and POMC, although AgRP tended to be lower and POMC higher in the malnutrition group. Regression analyses identified orexin A and PYY as significant predictors of malnutrition. Orexin A showed a positive correlation with emotional undereating. Sleep habits did not differ significantly between groups. Conclusions: Elevated orexin A and PYY levels may function as potential neuropeptidergic biomarkers of malnutrition. Their association with emotional eating highlights the psychobiological components of malnutrition. Further longitudinal studies are warranted to clarify causal mechanisms and support clinical translation.<\/jats:p>","DOI":"10.3390\/nu18030377","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T17:52:38Z","timestamp":1769190758000},"page":"377","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Relationship Between Orexin A, Peptide YY, AgRP, and POMC Levels and Sleep Disorders in Children with Malnutrition"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3494-1581","authenticated-orcid":false,"given":"Anna Carina","family":"Ergani","sequence":"first","affiliation":[{"name":"Department of Pediatric Gastroenterology, Konya City Hospital, 42020 Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8362-1934","authenticated-orcid":false,"given":"Mustafa Esad","family":"Tezcan","sequence":"additional","affiliation":[{"name":"Department of Child and Adolescent Mental Health, Faculty of Medicine, Selcuk University, 42250 Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8967-2924","authenticated-orcid":false,"given":"\u00dcmm\u00fcg\u00fcls\u00fcm","family":"Can","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Konya City Hospital, 42020 Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8319-2404","authenticated-orcid":false,"given":"Emine","family":"Arslan K\u0131l\u0131\u00e7o\u011flu","sequence":"additional","affiliation":[{"name":"Child Development Program, Department of Child Vocational, School of Health Services, KTO Karatay University, 42020 Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/nrn2092","article-title":"The neural circuit of orexin (hypocretin): Maintaining sleep and wakefulness","volume":"8","author":"Sakurai","year":"2007","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Taheri, S., Lin, L., Austin, D., Young, T., and Mignot, E. (2004). Short sleep duration is associated with reduced leptin, elevated ghrelin, and increased body mass index. PLoS Med., 1.","DOI":"10.1371\/journal.pmed.0010062"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1177\/0148607185009003309","article-title":"Nutritional depletions in critically ill children: Associations with physiologic instability and increased quantity of care","volume":"9","author":"Pollack","year":"1985","journal-title":"JPEN J. Parenter. Enteral Nutr."},{"key":"ref_4","unstructured":"UNICEF (2018). Malnutrition Rates Remain Alarming: Stunting Is Declining Too Slowly While Wasting Still Impacts the Lives of Far Too Many Young Children, UNICEF."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"R572","DOI":"10.1152\/ajpregu.00674.2010","article-title":"Local network regulation of orexin neurons in the lateral hypothalamus","volume":"301","author":"Burt","year":"2011","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1159\/000514960","article-title":"Sleep, orexin and cognition","volume":"Volume 45","author":"Toor","year":"2021","journal-title":"The Orexin System: Basic Science and Role in Sleep Pathology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.jsmc.2015.02.005","article-title":"Sleep-related breathing disorder, cognitive functioning, and behavioral-psychiatric syndromes in children","volume":"10","year":"2015","journal-title":"Sleep Med. Clin."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Owens, J. (2007). Classification and epidemiology of childhood sleep disorders. Principles and Practice of Pediatric Sleep Medicine, Elsevier Science.","DOI":"10.1016\/j.jsmc.2007.05.009"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1002\/phar.1686","article-title":"Management of sleep disorders in children with neurodevelopmental disorders: A review","volume":"36","author":"Blackmer","year":"2016","journal-title":"Pharmacotherapy"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1152\/physrev.00015.2004","article-title":"Hormonal regulation of food intake","volume":"85","author":"Stanley","year":"2005","journal-title":"Physiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"229","DOI":"10.5483\/BMBRep.2015.48.4.272","article-title":"Network of hypothalamic neurons that control appetite","volume":"48","author":"Sohn","year":"2015","journal-title":"BMB Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/nrg862","article-title":"Genetic approaches to studying energy balance: Perception and integration","volume":"3","author":"Barsh","year":"2002","journal-title":"Nat. Rev. Genet."},{"key":"ref_13","first-page":"9","article-title":"Age-related alterations in the central thermoregulatory responsiveness to \u03b1-MSH","volume":"49","author":"Tenk","year":"2015","journal-title":"J. Therm. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/0165-6147(89)90192-2","article-title":"Interaction of CCK with pancreatic acinar cells","volume":"10","author":"Jensen","year":"1989","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1159\/000514963","article-title":"Hypocretin\/orexin receptor pharmacology and sleep phases","volume":"Volume 45","author":"Sun","year":"2021","journal-title":"The Orexin System: Basic Science and Role in Sleep Pathology"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mogavero, M.P., Godos, J., Grosso, G., Caraci, F., and Ferri, R. (2023). Rethinking the role of orexin in the regulation of REM sleep and appetite. Nutrients, 15.","DOI":"10.3390\/nu15173679"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bou\u00e2ouda, H., and Jha, P.K. (2023). Orexin and MCH neurons: Regulators of sleep and metabolism. Front. Neurosci., 17.","DOI":"10.3389\/fnins.2023.1230428"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Liu, S., Wang, X., Zheng, Q., Gao, L., and Sun, Q. (2022). Sleep deprivation and central appetite regulation. Nutrients, 14.","DOI":"10.3390\/nu14245196"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1017\/S0954422423000264","article-title":"Sleep deprivation in development of obesity: Effects on appetite regulation, energy metabolism, and dietary choices","volume":"38","author":"Akhlaghi","year":"2023","journal-title":"Nutr. Res. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1111\/1469-7610.00792","article-title":"Development of the children\u2019s eating behaviour questionnaire","volume":"42","author":"Wardle","year":"2001","journal-title":"J. Child Psychol. Psychiatry"},{"key":"ref_21","first-page":"4","article-title":"Turkish adaptation of the Children\u2019s Eating Behaviour Questionnaire","volume":"12","author":"Yilmaz","year":"2011","journal-title":"Anatol. J. Psychiatry"},{"key":"ref_22","first-page":"151","article-title":"Validity and reliability of the Turkish version of the Children\u2019s Sleep Habits Questionnaire","volume":"11","author":"Fis","year":"2010","journal-title":"Alpha J. Psychiatry"},{"key":"ref_23","first-page":"539","article-title":"Qualitative and quantitative approaches to study adoption of sustainable agricultural practices: A research-note on mixed method approach","volume":"5","author":"Sattar","year":"2017","journal-title":"Int. J. Agric. Ext. Rural Dev."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Cohen, J. (2013). Statistical Power Analysis for the Behavioral Sciences, Routledge.","DOI":"10.4324\/9780203771587"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3803\/EnM.2013.28.4.288","article-title":"Effects of chronic restraint stress on body weight, food intake, and hypothalamic gene expressions in mice","volume":"28","author":"Jeong","year":"2013","journal-title":"Endocrinol. Metab."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"R7","DOI":"10.1677\/joe.0.160r007","article-title":"The effect of the orexins on food intake: Comparison with neuropeptide Y, melanin-concentrating hormone and galanin","volume":"160","author":"Edwards","year":"1999","journal-title":"J. Endocrinol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1097\/00001756-200006260-00034","article-title":"Centrally administered orexin\/hypocretin activates HPA axis in rats","volume":"11","author":"Kuru","year":"2000","journal-title":"NeuroReport"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Tsujino, N., and Sakurai, T. (2013). Role of orexin in modulating arousal, feeding, and motivation. Front. Behav. Neurosci., 7.","DOI":"10.3389\/fnbeh.2013.00028"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/bs.irn.2017.06.006","article-title":"Orexin\/hypocretin system: Role in food and drug overconsumption","volume":"136","author":"Barson","year":"2017","journal-title":"Int. Rev. Neurobiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"E726","DOI":"10.1152\/ajpendo.00660.2009","article-title":"Orexin activation precedes increased NPY expression, hyperphagia, and metabolic changes in response to sleep deprivation","volume":"298","author":"Martins","year":"2010","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.smrv.2011.03.005","article-title":"In search of lost sleep: Secular trends in the sleep time of school-aged children and adolescents","volume":"16","author":"Matricciani","year":"2012","journal-title":"Sleep Med. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1177\/10870547221149198","article-title":"The relationship between eating-attitudes and clinical characteristics, Agouti-related peptide, and other biochemical markers in adult-attention deficit hyperactivity disorder","volume":"27","author":"Ceyhun","year":"2023","journal-title":"J. Atten. Disord."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"174611","DOI":"10.1016\/j.ejphar.2021.174611","article-title":"AgRP\/NPY and POMC neurons in the arcuate nucleus and their potential role in treatment of obesity","volume":"915","author":"Vohra","year":"2022","journal-title":"Eur. J. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.1210\/en.2002-220338","article-title":"Agouti-related protein (AGRP) has a central inhibitory action on the hypothalamic-pituitary-thyroid (HPT) axis","volume":"143","author":"Fekete","year":"2002","journal-title":"Endocrinology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1016\/j.cell.2009.04.022","article-title":"Loss of GABAergic signaling by AgRP neurons to the parabrachial nucleus leads to starvation","volume":"137","author":"Wu","year":"2009","journal-title":"Cell"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1038\/nn.2739","article-title":"AGRP neurons are sufficient to orchestrate feeding behavior rapidly and without training","volume":"14","author":"Aponte","year":"2011","journal-title":"Nat. Neurosci."},{"key":"ref_37","first-page":"1079","article-title":"Leptin and insulin pathways in POMC and AgRP neurons that modulate energy balance and glucose homeostasis","volume":"13","author":"Varela","year":"2012","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1743-7075-4-18","article-title":"The role of proopiomelanocortin (POMC) neurones in feeding behaviour","volume":"4","author":"Millington","year":"2007","journal-title":"Nutr. Metab."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.neuron.2004.06.004","article-title":"Leptin receptor signaling in POMC neurons is required for normal body weight homeostasis","volume":"42","author":"Balthasar","year":"2004","journal-title":"Neuron"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1677\/joe.0.1720411","article-title":"Pro-opiomelanocortin processing in the hypothalamus: Impact on melanocortin signalling and obesity","volume":"172","author":"Pritchard","year":"2002","journal-title":"J. Endocrinol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1172\/JCI30227","article-title":"Gastrointestinal regulation of food intake","volume":"117","author":"Cummings","year":"2007","journal-title":"J. Clin. Investig."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1038\/nature00887","article-title":"Gut hormone PYY(3\u201336) physiologically inhibits food intake","volume":"418","author":"Batterham","year":"2002","journal-title":"Nature"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1159\/000079842","article-title":"Peptide YY directly inhibits ghrelin-activated neurons of the arcuate nucleus and reverses fasting-induced c-Fos expression","volume":"79","author":"Riediger","year":"2004","journal-title":"Neuroendocrinology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1523\/JNEUROSCI.1333-17.2017","article-title":"Restoring serotonergic homeostasis in the lateral hypothalamus rescues sleep disturbances induced by early-life obesity","volume":"38","author":"Gazea","year":"2018","journal-title":"J. Neurosci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.cmet.2005.08.003","article-title":"PYY3\u201336 inhibits the action potential firing activity of POMC neurons of arcuate nucleus through postsynaptic Y2 receptors","volume":"2","author":"Colmers","year":"2005","journal-title":"Cell Metab."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Menon, S., and Pe\u00f1alvo, J.L. (2019). Actions targeting the double burden of malnutrition: A scoping review. Nutrients, 12.","DOI":"10.3390\/nu12010081"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Ma, Z.F., Wang, C.W., and Lee, Y.Y. (2022). Malnutrition: A cause or a consequence of poverty?. Front. Public Health, 9.","DOI":"10.3389\/fpubh.2021.796435"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/S0140-6736(19)32473-0","article-title":"Economic effects of the double burden of malnutrition","volume":"395","author":"Nugent","year":"2020","journal-title":"Lancet"},{"key":"ref_49","unstructured":"Allison, D.B., and Baskin, M.L. (2009). Handbook of Assessment Methods for Eating Behaviors and Weight-Related Problems: Measures, Theory, and Research, Sage."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e12795","DOI":"10.1111\/ijpo.12795","article-title":"Psychometric properties of the Child Eating Behavior Questionnaire (CEBQ) in school-age children with overweight and obesity: A proposed three-factor structure","volume":"16","author":"Manzano","year":"2021","journal-title":"Pediatr. Obes."},{"key":"ref_51","first-page":"29","article-title":"Sleep regulation, physiology and development, sleep duration and patterns, and sleep hygiene in infants, toddlers, and preschool-age children","volume":"47","author":"Bathory","year":"2017","journal-title":"Curr. Probl. Pediatr. Adolesc. Health Care"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"113438","DOI":"10.1016\/j.physbeh.2021.113438","article-title":"Severe sleep restriction suppresses appetite independent of effects on appetite-regulating hormones in healthy young men without obesity","volume":"237","author":"Radcliffe","year":"2021","journal-title":"Physiol. Behav."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.ajcnut.2022.10.007","article-title":"The effect of modest changes in sleep on dietary intake and eating behavior in children: Secondary outcomes of a randomized crossover trial","volume":"117","author":"Morrison","year":"2023","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1002\/ncp.10588","article-title":"The use of visceral proteins as nutrition markers: An ASPEN position paper","volume":"36","author":"Evans","year":"2021","journal-title":"Nutr. Clin. Pract."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"370","DOI":"10.4240\/wjgs.v7.i12.370","article-title":"Hypoalbuminemia in colorectal cancer prognosis: Nutritional marker or inflammatory surrogate?","volume":"7","author":"Nazha","year":"2015","journal-title":"World J. Gastrointest. Surg."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Song, C., Sun, H., Wang, B., and Lu, H. (2021). Association between vitamin D status and undernutrition indices in children: A systematic review and meta-analysis of observational studies. Front. Pediatr., 9.","DOI":"10.3389\/fped.2021.665749"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e17500","DOI":"10.1097\/MD.0000000000017500","article-title":"Age-specific reference values for cerebrospinal fluid protein concentrations in children in southern China","volume":"98","author":"Liu","year":"2019","journal-title":"Medicine"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/3\/377\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T05:13:22Z","timestamp":1769490802000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/3\/377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,23]]},"references-count":57,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["nu18030377"],"URL":"https:\/\/doi.org\/10.3390\/nu18030377","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,23]]}}}