{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T07:05:50Z","timestamp":1784531150466,"version":"3.55.0"},"reference-count":34,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004504","name":"Research Council of Lithuania","doi-asserted-by":"crossref","award":["No. P-MIP-24-209"],"award-info":[{"award-number":["No. P-MIP-24-209"]}],"id":[{"id":"10.13039\/501100004504","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Objectives: The aim of this study was to investigate the effect of the interaction between aging and high-fat diet (HFD) or ketogenic diet (KD) on denervation-induced muscle atrophy. Methods: In this study, 6-, 19- and 27-month-old male mice were studied after 12 weeks\u2019 exposure to a regular chow diet, RD (kcal distribution: 13% fat, 57% carbohydrate, 30% protein), HFD (kcal distribution: 60% fat, 20% carbohydrates, 20% protein), or KD (kcal distribution: 80% fat, &lt;1% carbohydrates, 20% protein). Gastrocnemius (GAS) and soleus (SOL) muscles were left denervated during the last 6 weeks of this 12-week dietary intervention (n = 10 for each group). Results: Denervation-induced atrophy was greater (p &lt; 0.001) in GAS compared to SOL. There were no differences between type 1 and type 2 muscle fiber atrophy in adult SOL muscle. Muscle atrophy did not depend on the diet and was greater in adult than old mice. Both HFD and KD feeding reduced IGF-1 levels (p &lt; 0.01) in GAS muscle compared with the RD independently of age. Myostatin levels in GAS muscle increased (p &lt; 0.01) with age independently of the diets. Conclusions: Denervation-induced muscle atrophy does not depend on dietary fat intake and proceeds at a slower rate in old mice compared to adult mice.<\/jats:p>","DOI":"10.3390\/nu18030478","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T14:12:46Z","timestamp":1770041566000},"page":"478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Aging in Combination with High-Fat or Ketogenic Diet on Skeletal Muscle Atrophy Following Denervation in C57BL\/6J Mice"],"prefix":"10.3390","volume":"18","author":[{"given":"Mantas","family":"Dirmontas","sequence":"first","affiliation":[{"name":"Department of Health Promotion and Rehabilitation, Lithuanian Sports University, LT-44221 Kaunas, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9522-6050","authenticated-orcid":false,"given":"Petras","family":"Minderis","sequence":"additional","affiliation":[{"name":"Institute of Sport Science and Innovations, Lithuanian Sports University, LT-44221 Kaunas, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4737-5817","authenticated-orcid":false,"given":"Aivaras","family":"Ratkevicius","sequence":"additional","affiliation":[{"name":"Department of Health Promotion and Rehabilitation, Lithuanian Sports University, LT-44221 Kaunas, Lithuania"},{"name":"Sports and Exercise Medicine Centre, Queen Mary University of London, London E1 4NS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.hcl.2013.04.002","article-title":"Nerve physiology","volume":"29","author":"Menorca","year":"2013","journal-title":"Hand Clin."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Rumora, A.E., Guo, K., Hinder, L.M., O\u2019Brien, P.D., Hayes, J.M., Hur, J., and Feldman, E.L. 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