{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:47:37Z","timestamp":1790729257679,"version":"4.1.0"},"reference-count":42,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanxi Health Committee","award":["zyytd2024038"],"award-info":[{"award-number":["zyytd2024038"]}]},{"DOI":"10.13039\/501100004480","name":"Natural Science Foundation of Shanxi Province","doi-asserted-by":"crossref","award":["202203021221259"],"award-info":[{"award-number":["202203021221259"]}],"id":[{"id":"10.13039\/501100004480","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Objectives: The main purpose of this study was to evaluate the potential preventive effect of Total Flavonoids from Sea Buckthorn Leaves (Fla) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice from an integrated perspective of \u201cgut microbiota\u2013host interaction,\u201d and to elucidate its regulatory mechanism within the microbiota\u2013metabolite\u2013barrier\u2013immune axis. Methods: A DSS-induced UC mouse model was established, and mice were randomly assigned into normal control, model, mesalazine, and Fla low, middle, and high\u2013dose groups. Disease severity, colonic barrier integrity, inflammatory cytokines, gut microbiota composition, and short-chain fatty acid levels were evaluated using histopathological, molecular biological, and metabolomic analyses. Result: Fla significantly ameliorated colonic damage and other pathological symptoms. It enhanced intestinal barrier integrity by upregulating ZO-1, E-cadherin, and MUC2 expression and suppressed inflammation by reducing TNF-\u03b1 and IL-6 levels. Furthermore, Fla reshaped gut microbiota, increasing beneficial Akkermansia abundance, and elevated short-chain fatty acid (SCFA) production, with the most pronounced effects observed at the middle dose. Conclusions: In summary, in this preventive model, Fla synergistically enhances intestinal barrier function and suppresses excessive local inflammatory responses by reshaping the gut microbiota and promoting SCFA production, ultimately alleviating UC. This study provides scientific evidence for the high-value utilization of sea buckthorn leaves (a by-product) and offers new insights for developing microbiome-based preventive strategies for UC.<\/jats:p>","DOI":"10.3390\/nu18040620","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T16:09:32Z","timestamp":1770998972000},"page":"620","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["From Microbiota to Defense: The Preventive Effect and Mechanism of Total Flavonoids from Sea Buckthorn Leaves in DSS-Induced Colitis"],"prefix":"10.3390","volume":"18","author":[{"given":"Ying","family":"Guo","sequence":"first","affiliation":[{"name":"The Fifth Clinical Medical College, Shanxi Medical University, Taiyuan 030012, China"},{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qihuiru","family":"Wang","sequence":"additional","affiliation":[{"name":"The Fifth Clinical Medical College, Shanxi Medical University, Taiyuan 030012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyu","family":"Guo","sequence":"additional","affiliation":[{"name":"The Fifth Clinical Medical College, Shanxi Medical University, Taiyuan 030012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongye","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Fifth Clinical Medical College, Shanxi Medical University, Taiyuan 030012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linjun","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5139-2057","authenticated-orcid":false,"given":"Xiaoqiong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4423-5085","authenticated-orcid":false,"given":"Xiangyu","family":"Bian","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4662-7207","authenticated-orcid":false,"given":"Jinjun","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijun","family":"Ma","sequence":"additional","affiliation":[{"name":"The Fifth Clinical Medical College, Shanxi Medical University, Taiyuan 030012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/j.tips.2024.08.003","article-title":"Ulcerative colitis: Clinical biomarkers, therapeutic targets, and emerging treatments","volume":"45","author":"Wangchuk","year":"2024","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/S0140-6736(23)00966-2","article-title":"Ulcerative colitis","volume":"402","author":"Honap","year":"2023","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s43556-024-00207-w","article-title":"Ulcerative colitis: Molecular insights and intervention therapy","volume":"5","author":"Liang","year":"2024","journal-title":"Mol. Biomed."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"106406","DOI":"10.3748\/wjg.v31.i19.106406","article-title":"Comparative study on the pathogenesis of Crohn\u2019s disease and ulcerative colitis","volume":"31","author":"Yang","year":"2025","journal-title":"World J. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1111\/apt.18495","article-title":"Meta-Analysis: Exclusive Enteral Nutrition in Adults with Ulcerative Colitis","volume":"61","author":"Chu","year":"2025","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.3748\/wjg.v29.i14.2078","article-title":"Role of prebiotics, probiotics, and synbiotics in management of inflammatory bowel disease: Current perspectives","volume":"29","author":"Roy","year":"2023","journal-title":"World J. Gastroenterol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Martyniak, A., Medy\u0144ska-Prz\u0119czek, A., W\u0119drychowicz, A., Skocze\u0144, S., and Tomasik, P.J. (2021). Prebiotics, Probiotics, Synbiotics, Paraprobiotics and Postbiotic Compounds in IBD. Biomolecules, 11.","DOI":"10.3390\/biom11121903"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wang, X., Yuan, W., Yang, C., Wang, Z., Zhang, J., Xu, D., Sun, X., and Sun, W. (2024). Emerging role of gut microbiota in autoimmune diseases. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1365554"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1186\/s12964-024-01611-z","article-title":"The development of probiotics and prebiotics therapy to ulcerative colitis: A therapy that has gained considerable momentum","volume":"22","author":"Guo","year":"2024","journal-title":"Cell Commun. Signal."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Jadhav, A., Jagtap, S., Vyavahare, S., Sharbidre, A., and Kunchiraman, B. (2023). Reviewing the potential of probiotics, prebiotics and synbiotics: Advancements in treatment of ulcerative colitis. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1268041"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"230014","DOI":"10.1098\/rsob.230014","article-title":"Short chain fatty acids: Key regulators of the local and systemic immune response in inflammatory diseases and infections","volume":"13","author":"Ney","year":"2023","journal-title":"Open Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.immuni.2013.12.007","article-title":"Activation of Gpr109a, receptor for niacin and the commensal metabolite butyrate, suppresses colonic inflammation and carcinogenesis","volume":"40","author":"Singh","year":"2014","journal-title":"Immunity"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Maniangat Luke, A., Kumari, N.S., Hegde, M.N., and Hegde, N.N. (2025). Metabolic interplay of SCFA\u2019s in the gut and oral microbiome: A link to health and disease. Front. Oral Health, 6.","DOI":"10.3389\/froh.2025.1646382"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2290213","DOI":"10.1080\/07853890.2023.2290213","article-title":"Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota","volume":"55","author":"Li","year":"2023","journal-title":"Ann. Med."},{"key":"ref_15","first-page":"564","article-title":"Ulcerative Colitis-Diagnostic and Therapeutic Algorithms","volume":"117","author":"Kucharzik","year":"2020","journal-title":"Dtsch. Arztebl. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1016\/S0140-6736(16)32126-2","article-title":"Ulcerative colitis","volume":"389","author":"Ungaro","year":"2017","journal-title":"Lancet"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"114086","DOI":"10.1016\/j.biopha.2022.114086","article-title":"The role and mechanism of flavonoid herbal natural products in ulcerative colitis","volume":"158","author":"Xue","year":"2023","journal-title":"Biomed. Pharmacother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"132531","DOI":"10.1016\/j.foodchem.2022.132531","article-title":"Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity","volume":"383","author":"Shen","year":"2022","journal-title":"Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e13724","DOI":"10.1111\/jfbc.13724","article-title":"Flavonoids from sea buckthorn: A review on phytochemistry, pharmacokinetics and role in metabolic diseases","volume":"45","author":"Liu","year":"2021","journal-title":"J. Food Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"142591","DOI":"10.1016\/j.foodchem.2024.142591","article-title":"Sea buckthorn polyphenols on gastrointestinal health and the interactions with gut microbiota","volume":"469","author":"Yuan","year":"2025","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"103746","DOI":"10.1016\/j.redox.2025.103746","article-title":"Anemoside B4 alleviates ulcerative colitis by attenuating intestinal oxidative stress and NLRP3 inflammasome via activating aryl hydrocarbon receptor through remodeling the gut microbiome and metabolites","volume":"85","author":"Wu","year":"2025","journal-title":"Redox Biol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Xu, X., Wu, J., Jin, Y., Huang, K., Zhang, Y., and Liang, Z. (2023). Both Saccharomyces boulardii and Its Postbiotics Alleviate Dextran Sulfate Sodium-Induced Colitis in Mice, Association with Modulating Inflammation and Intestinal Microbiota. Nutrients, 15.","DOI":"10.3390\/nu15061484"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Jin, Y., Wu, J., Huang, K., and Liang, Z. (2024). Heat-Killed Saccharomyces boulardii Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis by Restoring the Intestinal Barrier, Reducing Inflammation, and Modulating the Gut Microbiota. Nutrients, 16.","DOI":"10.3390\/nu16050702"},{"key":"ref_24","first-page":"6547","article-title":"Elucidating the Significance of Zonulin in the Pathogenesis of Chronic Inflammatory Disorders: Emphasis on Intestinal Barrier Function and Tight Junction Regulation","volume":"32","author":"Mousavi","year":"2024","journal-title":"Curr. Med. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101486","DOI":"10.1016\/j.jcmgh.2025.101486","article-title":"Fibroblast Growth Factor 20 Attenuates Colitis by Restoring Impaired Intestinal Epithelial Barrier Integrity and Modulating Macrophage Polarization via S100A9 in an NF-\u03baB-Dependent Manner","volume":"19","author":"Zhen","year":"2025","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/s41419-021-03843-0","article-title":"Epithelial PBLD attenuates intestinal inflammatory response and improves intestinal barrier function by inhibiting NF-\u03baB signaling","volume":"12","author":"Chen","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Mazurek-Mochol, M., Bonsmann, T., Mochol, M., Poniewierska-Baran, A., and Pawlik, A. (2024). The Role of Interleukin 6 in Periodontitis and Its Complications. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25042146"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"13866","DOI":"10.1038\/s41598-025-98869-1","article-title":"Exploring the mechanisms of Chaige Kangyi Recipe in treating recurrent pregnancy loss with insulin resistance","volume":"15","author":"Wu","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Reza, M.I., and Ambhore, N.S. (2025). Inflammation in Asthma: Mechanistic Insights and the Role of Biologics in Therapeutic Frontiers. Biomedicines, 13.","DOI":"10.20944\/preprints202504.2163.v1"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5526","DOI":"10.1038\/s41598-022-09559-1","article-title":"Molecular mechanisms of Huanglian jiedu decoction on ulcerative colitis based on network pharmacology and molecular docking","volume":"12","author":"Yang","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1007\/s00018-022-04649-9","article-title":"Deleting interleukin-10 from myeloid cells exacerbates atherosclerosis in Apoe-\/- mice","volume":"80","author":"Orecchioni","year":"2022","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s12672-025-01949-x","article-title":"Deciphering the metabolic landscape of colorectal cancer through the lens of AhR-mediated intestinal inflammation","volume":"16","author":"Swain","year":"2025","journal-title":"Discov. Oncol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Carretta, M.D., Quiroga, J., L\u00f3pez, R., Hidalgo, M.A., and Burgos, R.A. (2021). Participation of Short-Chain Fatty Acids and Their Receptors in Gut Inflammation and Colon Cancer. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.662739"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Coccia, C., Bonomi, F., Lo Cricchio, A., Russo, E., Peretti, S., Bandini, G., Lepri, G., Bartoli, F., Moggi-Pignone, A., and Guiducci, S. (2024). The Potential Role of Butyrate in the Pathogenesis and Treatment of Autoimmune Rheumatic Diseases. Biomedicines, 12.","DOI":"10.3390\/biomedicines12081760"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"101226","DOI":"10.1016\/j.isci.2020.101226","article-title":"Microbiota-Sourced Purines Support Wound Healing and Mucous Barrier Function","volume":"23","author":"Lee","year":"2020","journal-title":"iScience"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"128317","DOI":"10.1016\/j.micres.2025.128317","article-title":"Akkermansia muciniphila: A key player in gut microbiota-based disease modulation","volume":"301","author":"Shaheen","year":"2025","journal-title":"Microbiol. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e04249","DOI":"10.1002\/advs.202504249","article-title":"The Interplay between the Gut and Ketogenic Diets in Health and Disease","volume":"12","author":"Mu","year":"2025","journal-title":"Adv. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4844","DOI":"10.1038\/s41467-020-18696-y","article-title":"Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila","volume":"11","author":"Trastoy","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"9341","DOI":"10.1038\/s41467-025-64395-x","article-title":"Red palm olein biscuit supplementation modulates gut microbiota in vitamin A deficient rural Malaysian schoolchildren: A randomised controlled trial","volume":"16","author":"Tan","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6634821","DOI":"10.1155\/2021\/6634821","article-title":"Gut Immunity and Microbiota Dysbiosis Are Associated with Altered Bile Acid Metabolism in LPS-Challenged Piglets","volume":"2021","author":"Xiao","year":"2021","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Bondy, S.C. (2023). The Hormesis Concept: Strengths and Shortcomings. Biomolecules, 13.","DOI":"10.3390\/biom13101512"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Meneguzzo, F., Zabini, F., and Albanese, L. (2026). Green Extraction at Scale: Hydrodynamic Cavitation for Bioactive Recovery and Protein Functionalization\u2014A Narrative Review. Molecules, 31.","DOI":"10.3390\/molecules31010192"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/4\/620\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T05:19:31Z","timestamp":1771132771000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/4\/620"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,13]]},"references-count":42,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["nu18040620"],"URL":"https:\/\/doi.org\/10.3390\/nu18040620","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,13]]}}}