{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:05:49Z","timestamp":1785420349628,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20230610"],"award-info":[{"award-number":["BK20230610"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"award":["BK20230610"],"award-info":[{"award-number":["BK20230610"]}],"id":[{"id":"https:\/\/ror.org\/004svx814","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Health Commission of Nantong City","award":["MS2025074"],"award-info":[{"award-number":["MS2025074"]}]},{"award":["MS2025074"],"award-info":[{"award-number":["MS2025074"]}],"id":[{"id":"https:\/\/ror.org\/01xncyx73","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018557","name":"Nantong Science and Technology Project","doi-asserted-by":"publisher","award":["MS2024057"],"award-info":[{"award-number":["MS2024057"]}],"id":[{"id":"10.13039\/501100018557","id-type":"DOI","asserted-by":"publisher"}]},{"award":["MS2024057"],"award-info":[{"award-number":["MS2024057"]}],"id":[{"id":"https:\/\/ror.org\/02tx34m84","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Visceral pain is the primary symptom of functional gastrointestinal disorders, yet its spinal molecular mechanisms remain poorly defined. Methods: Using a 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced chronic inflammatory visceral pain model, the role of the spinal CXCL10\/CXCR3\/ERK signaling axis and the analgesic effect of docosahexaenoic acid (DHA) were investigated. Results: TNBS significantly upregulated CXCL10 and CXCR3 in spinal dorsal horn neurons and increased ERK phosphorylation. Intrathecal CXCL10-neutralizing antibody or CXCR3 antagonist NBI-74330 reduced visceral hypersensitivity and suppressed spinal ERK activation in TNBS mice. Exogenous CXCL10 induced CXCR3-dependent hyperalgesia and ERK phosphorylation in the spinal cord. Intrathecal DHA attenuated TNBS-induced visceral pain, downregulated spinal CXCL10\/CXCR3 expression, and inhibited ERK signaling. In Neuro-2a cells, DHA also blocked LPS-induced activation of the same pathway. Conclusions: This study suggests that the analgesic effect of DHA may involve the inhibition of the spinal CXCL10\/CXCR3\/ERK signaling pathway.<\/jats:p>","DOI":"10.3390\/nu18071113","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T15:20:47Z","timestamp":1774884047000},"page":"1113","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Docosahexaenoic Acid Attenuates Visceral Pain by Suppressing Spinal CXCL10\/CXCR3\/ERK Signaling"],"prefix":"10.3390","volume":"18","author":[{"given":"Xi","family":"Yin","sequence":"first","affiliation":[{"name":"Research Institute of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anqi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Research Institute of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Han","sequence":"additional","affiliation":[{"name":"Research Institute of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Qu","sequence":"additional","affiliation":[{"name":"Research Institute of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianya","family":"Zhao","sequence":"additional","affiliation":[{"name":"Research Institute of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Gong","sequence":"additional","affiliation":[{"name":"Research Institute of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorong","family":"Luo","sequence":"additional","affiliation":[{"name":"Research Institute of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8579-0632","authenticated-orcid":false,"given":"Xu","family":"Li","sequence":"additional","affiliation":[{"name":"Research Institute of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Lu","sequence":"additional","affiliation":[{"name":"Research Institute of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e70255","DOI":"10.1111\/cns.70255","article-title":"Discovery of Novel Pain Regulators Through Integration of Cross-Species High-Throughput Data","volume":"31","author":"Chen","year":"2025","journal-title":"CNS Neurosci. 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