{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T13:02:25Z","timestamp":1776949345578,"version":"3.51.4"},"reference-count":80,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Constipation is a common gastrointestinal (GI) state for which probiotics have shown promise as a relief. This study examined the laxative effects of the strain Bifidobacterium animalis subsp. lactis CECT 8145 (BPL1\u00ae) in a loperamide-induced rat model of constipation. Methods: Fifty-nine rats were divided into control and loperamide-induced constipation groups. Animals received a 3-day intervention with either placebo or probiotic BPL1\u00ae at two doses: 1.5 \u00d7 108 CFU (colony-forming units) (low) and 3 \u00d7 109 CFU (high). The study assessed several parameters to determine the probiotic\u2019s effect, including: stool and gut characteristics, gastrointestinal transit time (GTT), gene expression and gut microbiome composition. Results: While loperamide significantly decreased stool number, weight and humidity, BPL1\u00ae supplementation effectively restored these parameters, being more pronounced at a high dose. Microbiome analysis showed that BPL1\u00ae at a low dose reduced the abundance of Muribaculaceae and Muribaculum gordoncarteri, associated with constipation. In addition, Muribaculaceae abundance was negatively correlated with stool humidity. Functional microbiome profiling indicated that BPL1\u00ae suppressed pathways related to mucin degradation, vancomycin resistance and isoleucine biosynthesis while promoting L-lactate and pyridoxal-P (vitamin B6) biosynthesis, which may support gut motility and barrier integrity. Conclusions:Bifidobacterium animalis subsp. lactis BPL1\u00ae exhibits potential as a functional probiotic for relieving constipation through improving stool excretion and consistency, inducing taxonomic changes and beneficial functional modulation of the intestinal microbiome. These findings justify further investigation into the mechanisms of BPL1\u00ae as a probiotic for constipation management.<\/jats:p>","DOI":"10.3390\/nu18081237","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T15:47:02Z","timestamp":1776181622000},"page":"1237","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bifidobacterium animalis subsp. lactis CECT 8145 BPL1\u00ae Laxative Effects in Loperamide-Induced Constipated SD Rats"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0948-7615","authenticated-orcid":false,"given":"Andrea","family":"Rodenes-Gavidia","sequence":"first","affiliation":[{"name":"ADM R&D Health & Wellness, Parque Cient\u00edfico Universitat de Val\u00e8ncia, Carrer del Catedr\u00e0tic Agust\u00edn Escardino Benlloch, 9, 46980 Paterna, Spain"},{"name":"PhD Programme in Biotechnology, Universitat Polit\u00e8cnica de Val\u00e8ncia, Cami de Vera s\/n, 46022 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0416-7102","authenticated-orcid":false,"given":"Anna","family":"Mas-Capdevilla","sequence":"additional","affiliation":[{"name":"Eurecat, Centre Tecnol\u00f2gic de Catalunya, Technological Unit of Nutrition and Health, Avinguda Universitat 1, 43204 Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2867-5754","authenticated-orcid":false,"given":"Adri\u00e1n","family":"Florit","sequence":"additional","affiliation":[{"name":"ADM R&D Health & Wellness, Parque Cient\u00edfico Universitat de Val\u00e8ncia, Carrer del Catedr\u00e0tic Agust\u00edn Escardino Benlloch, 9, 46980 Paterna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Enrique L\u00f3pez","sequence":"additional","affiliation":[{"name":"ADM R&D Health & Wellness, Parque Cient\u00edfico Universitat de Val\u00e8ncia, Carrer del Catedr\u00e0tic Agust\u00edn Escardino Benlloch, 9, 46980 Paterna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9425-1115","authenticated-orcid":false,"given":"Daniel","family":"Gonz\u00e1lez-Hedstr\u00f6m","sequence":"additional","affiliation":[{"name":"ADM R&D Health & Wellness, Parque Cient\u00edfico Universitat de Val\u00e8ncia, Carrer del Catedr\u00e0tic Agust\u00edn Escardino Benlloch, 9, 46980 Paterna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1939-5642","authenticated-orcid":false,"given":"Araceli","family":"Lamelas","sequence":"additional","affiliation":[{"name":"ADM R&D Health & Wellness, Parque Cient\u00edfico Universitat de Val\u00e8ncia, Carrer del Catedr\u00e0tic Agust\u00edn Escardino Benlloch, 9, 46980 Paterna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5689-8399","authenticated-orcid":false,"given":"Patricia","family":"Martorell","sequence":"additional","affiliation":[{"name":"ADM R&D Health & Wellness, Parque Cient\u00edfico Universitat de Val\u00e8ncia, Carrer del Catedr\u00e0tic Agust\u00edn Escardino Benlloch, 9, 46980 Paterna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6891-3573","authenticated-orcid":false,"given":"Empar","family":"Chenoll","sequence":"additional","affiliation":[{"name":"ADM R&D Health & Wellness, Parque Cient\u00edfico Universitat de Val\u00e8ncia, Carrer del Catedr\u00e0tic Agust\u00edn Escardino Benlloch, 9, 46980 Paterna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8676-5081","authenticated-orcid":false,"given":"Vanessa","family":"Illescas-Armijo","sequence":"additional","affiliation":[{"name":"ADM R&D Health & Wellness, Parque Cient\u00edfico Universitat de Val\u00e8ncia, Carrer del Catedr\u00e0tic Agust\u00edn Escardino Benlloch, 9, 46980 Paterna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9340-6207","authenticated-orcid":false,"given":"Juan","family":"Mart\u00ednez-Blanch","sequence":"additional","affiliation":[{"name":"ADM R&D Health & Wellness, Parque Cient\u00edfico Universitat de Val\u00e8ncia, Carrer del Catedr\u00e0tic Agust\u00edn Escardino Benlloch, 9, 46980 Paterna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8026-7375","authenticated-orcid":false,"given":"Anna","family":"Antol\u00edn","sequence":"additional","affiliation":[{"name":"Eurecat, Centre Tecnol\u00f2gic de Catalunya, Technological Unit of Nutrition and Health, Avinguda Universitat 1, 43204 Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5705-7807","authenticated-orcid":false,"given":"Juan Mar\u00eda","family":"Alcaide-Hidalgo","sequence":"additional","affiliation":[{"name":"Eurecat, Centre Tecnol\u00f2gic de Catalunya, Technological Unit of Nutrition and Health, Avinguda Universitat 1, 43204 Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0924-4920","authenticated-orcid":false,"given":"Roger","family":"Marin\u00e9-Casad\u00f3","sequence":"additional","affiliation":[{"name":"Eurecat, Centre Tecnol\u00f2gic de 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