{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T11:22:41Z","timestamp":1776424961197,"version":"3.51.2"},"reference-count":58,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Danone Nutrition Center Dietary Nutrition Research and Education Fund","award":["DIC2021-02"],"award-info":[{"award-number":["DIC2021-02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Accurate estimation of water and energy requirements is fundamental for establishing dietary reference values in young adults. However, evidence integrating objectively measured energy expenditure with detailed water turnover components remains limited in Chinese populations. Objectives: To quantify water intake, water loss, and energy expenditure in healthy young college students, and to examine how energy metabolism is associated with specific components of water turnover under free-living conditions. Methods: Twenty-one healthy adults aged 18\u201325 years participated in a 14-day observational study conducted in Beijing, China. Total energy expenditure (TEE) was measured over 14 days using the doubly labeled water (DLW) method. Physical activity was monitored over 7 consecutive days using a triaxial accelerometer. Water intake was assessed using multiple methods: water from beverages (including plain drinking water and other beverages) was recorded over 7 days using 24 h fluid intake records, while water from food was measured during days 5\u20137 using weighed food records combined with duplicate portion and direct drying methods. Urinary and fecal water loss were quantified using 24 h collections conducted during days 5\u20137. Metabolic water production and insensible water losses were estimated using established physiological equations. Multivariable linear regression analyses were conducted to examine associations between energy-related variables and components of water turnover. Results: Mean total daily water intake was 3023 mL, with water from beverages accounting for 54.1%, water from food for 36.7%, and metabolic water for 9.1%. Mean total daily water loss was 1931 mL, predominantly from urinary excretion (81.0%). DLW-measured TEE averaged 2018.6 kcal\/day and was higher in males than in females. Most regression models examining total water intake and beverage-derived water were not statistically significant, and no consistent associations were observed between these variables and total energy intake, TEE, or PAEE. In contrast, TEE was positively associated with metabolic water production and respiratory water loss (both p &lt; 0.001). Significant associations with total energy intake were observed for water from food and fecal water loss (both p &lt; 0.01), whereas other water intake components showed no significant associations. Conclusions: In young adults, energy metabolism appears to be more closely associated with physiologically regulated components of water turnover than with voluntary water intake. These findings suggest a divergence between endogenous and behaviorally regulated pathways of water turnover and highlight the importance of considering component-specific water dynamics when examining hydration and energy balance, although confirmation in larger studies is warranted.<\/jats:p>","DOI":"10.3390\/nu18081268","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T10:11:04Z","timestamp":1776420664000},"page":"1268","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Water and Energy Turnover in Chinese Young Adults: A Doubly Labeled Water Study of Metabolic Coupling"],"prefix":"10.3390","volume":"18","author":[{"given":"Xing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, 38 Xue Yuan Road, Haidian District, Beijing 100191, China"},{"name":"Clinical Nutrition Department, Ningbo Medical Center Li Huili Hospital, 1111 Jiangnan Road, Yinzhou District, Ningbo 315000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, 38 Xue Yuan Road, Haidian District, Beijing 100191, China"},{"name":"Office of National Nutrition Plan, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, 27 Nanwei Road, Xicheng District, Beijing 100050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianfen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, 38 Xue Yuan Road, Haidian District, Beijing 100191, China"},{"name":"Department of Student Nutrition, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, 27 Nanwei Road, Xicheng District, Beijing 100050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7713-5980","authenticated-orcid":false,"given":"Na","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, 38 Xue Yuan Road, Haidian District, Beijing 100191, China"},{"name":"Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, 38 Xue Yuan Road, Haidian District, Beijing 100191, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1152\/physrev.2000.80.2.649","article-title":"Human Body Composition: In Vivo Methods","volume":"80","author":"Ellis","year":"2000","journal-title":"Physiol. 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