{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:21:26Z","timestamp":1785410486404,"version":"3.56.0"},"reference-count":38,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondo Sectorial de Investigaci\u00f3n y Seguridad Social Project","award":["A3-S-37825"],"award-info":[{"award-number":["A3-S-37825"]}]},{"award":["A3-S-37825"],"award-info":[{"award-number":["A3-S-37825"]}],"id":[{"id":"https:\/\/ror.org\/059ex5q34","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Overweight and obesity are chronic diseases that result from complex interactions including genetics, environment, eating behaviors, and limited access to a healthy diet. Amaranth protein (AmProt) has several health benefits, but no studies have examined its effects on the modulation of children\u2019s gut microbiota. The work aimed to analyze serum levels and changes in gut microbiota in children aged 8\u201310 years with different body mass index (BMI) values after supplementation with AmProt. Methods: Participating children were allocated into three groups according to their BMI: normal weight (NW), overweight (OW), and with obesity (OB). Children received AmProt for 90 days. Levels of fasting blood glucose, cholesterol, triglycerides, and insulin were analyzed before and after diet supplementation. HOMA-IR and adinopectin\/leptin ratio were evaluated. Feces were collected and metagenome analysis was carried out. Results: No changes in glucose levels were observed across groups and treatments; however, cholesterol and triglycerides levels tended to decrease. The HOMA-IR value increased in relation to BMI and no changes were observed after treatment. Firmicutes were highly abundant in all groups. The lower abundance of Ruminococcus was observed in the OW and OB groups. In the OW group, Blautia, Butyricicoccus, and Roseburia were also observed in increased abundance. In all groups, AmProt consumption tended to increase the abundance of Coproccus, Prevotella, and Collinsella. Conclusions: Supplementation of the children\u2019s diet with AmProt showed an improvement in serum cholesterol and triglyceride levels, which could be related to changes in the microbiota related to lipid metabolism.<\/jats:p>","DOI":"10.3390\/nu18111690","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T11:02:51Z","timestamp":1779793371000},"page":"1690","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary Supplementation with Amaranth Protein Isolate Modulates the Gut Microbiota in Children with Overweight and Obesity: A Nonrandomized Trial"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4999-036X","authenticated-orcid":false,"given":"Ana P.","family":"Barba-de la Rosa","sequence":"first","affiliation":[{"name":"Molecular Biology Division, Potosino Institute of Scientific and Technological Research A.C., Camino a la Presa San Jose 2055, Lomas 4a Seccion, San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5679-1671","authenticated-orcid":false,"given":"Samuel","family":"Trevi\u00f1o","sequence":"additional","affiliation":[{"name":"Institute of Physiology, Meritorious Autonomous University of Puebla, Prolongaci\u00f3n De la 14 Sur 6301, Cd. Universitaria, Puebla 72592, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0201-695X","authenticated-orcid":false,"given":"Cesar\u00e9","family":"Ovando-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"SECIHTI-National Supercomputing Center, Potosino Institute of Scientific and Technological Research A.C., Camino a la Presa San Jose 2055, Lomas 4a Seccion, San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3347-3499","authenticated-orcid":false,"given":"Antonio","family":"De Le\u00f3n-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Molecular Biology Division, Potosino Institute of Scientific and Technological Research A.C., Camino a la Presa San Jose 2055, Lomas 4a Seccion, San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9360-8781","authenticated-orcid":false,"given":"Oscar de Jes\u00fas","family":"Calva-Cruz","sequence":"additional","affiliation":[{"name":"Molecular Biology Division, Potosino Institute of Scientific and Technological Research A.C., Camino a la Presa San Jose 2055, Lomas 4a Seccion, San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0315-7591","authenticated-orcid":false,"given":"Alberto","family":"Barrera-Pacheco","sequence":"additional","affiliation":[{"name":"Molecular Biology Division, Potosino Institute of Scientific and Technological Research A.C., Camino a la Presa San Jose 2055, Lomas 4a Seccion, San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Espitia-Rangel","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investigaciones Forestales Agr\u00edcolas y Pecuarias, Campo Experimental Valle de M\u00e9xico, Km 13.5 Carr. Los Reyes-Texcoco, Texcoco 56250, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1007\/s11121-021-01316-6","article-title":"Interventions to prevent obesity in Mexican children and adolescents: Systematic Review","volume":"23","year":"2022","journal-title":"Prev. Sci."},{"key":"ref_2","unstructured":"NCD Risk Factor Collaboration (NCD-RisC) (2024). Worldwide trends in underweight and obesity from 1990 to 2022: A pooled analysis of 3663 population-representative studies with 222 million children, adolescents, and adults. Lancet, 403, 1027\u20131050."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100444","DOI":"10.1016\/j.metop.2026.100444","article-title":"Obesity-induced cognitive impairment: Underlying mechanisms and therapeutic prospects","volume":"29","author":"Liu","year":"2026","journal-title":"Metabol. Open"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1038\/s41522-024-00610-9","article-title":"Unlocking gut-liver-brain axis communication metabolites: Energy metabolism, immunity and barriers","volume":"10","author":"Sun","year":"2024","journal-title":"Npfj Biofilms Microbiomes"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e00479-24","DOI":"10.1128\/jb.00479-24","article-title":"Unravelling the gut microbiota\u2019s role in obesity: Key metabolites, microbial species, and therapeutic insights","volume":"207","author":"Iqbal","year":"2025","journal-title":"J. Bacteriol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.nutos.2023.10.008","article-title":"Effects of a high-protein diet and calcium caseinate supplementation on satiety perception and weight in children with overweight and obesity: A randomized clinical trial","volume":"52","author":"Feferbaum","year":"2023","journal-title":"Clin. Nutr. Open Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Wu, S., Bhat, Z.F., Gounder, R.S., Mohamed Ahmed, I.A., Al-Juhaimi, F.Y., Ding, Y., and Bekhit, A.E.-D.A. (2022). Effect of dietary protein and processing on gut microbiota- A systematic review. Nutrients, 4.","DOI":"10.3390\/nu14030453"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"112405","DOI":"10.1016\/j.foodres.2022.112405","article-title":"Amaranth proteins and peptides: Biological properties and food uses","volume":"164","author":"Zhu","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_9","unstructured":"Barba-de la Rosa, A.P., De Le\u00f3n-Rodr\u00edguez, A., Calva-Cruz, O.J., and Garc\u00eda-Campoy, A.H. (2025). Proceso Basado en Fluidos Supercr\u00edticos Para el Aprovechamiento de Semillas y Obtenci\u00f3n de Subproductos de Alto Valor Agregado. (429295), Mexican Patent."},{"key":"ref_10","unstructured":"FANTA (2025, May 15). Food and Nutrition Technical Assistance. BMI and BMI-for-Age Look-up Tables for Children and Adolescents 5\u201318 Years of Age and BMI Look-up Tables for Non-Pregnant, Non-Lactating Adults \u2265 19 Years of Age. Available online: https:\/\/www.fantaproject.org\/sites\/default\/files\/resources\/FANTA-BMI-charts-Jan2013-ENG_0.pdf."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Heo, A.S., and Lee, J.C. (2025). Development of a metabolic syndrome prediction model using HOMA-IR and multivariate factors. Appl. Sci., 15.","DOI":"10.3390\/app15062985"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s12011-013-9656-1","article-title":"Seven dietary minerals (Ca, P, Mg, Fe, Zn, Cu, and Mn) and their relationships with blood pressure and blood lipids in healthy adults with self-selected diet","volume":"153","author":"Kim","year":"2013","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Yang, Y., Fukui, R., Jia, H., and Kato, H. (2021). Amaranth supplementation improves hepatic lipid dysmetabolism and modulates gut microbiota in mice fed a high-fat diet. Foods, 10.","DOI":"10.3390\/foods10061259"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"100","DOI":"10.4274\/jcrpe.v2i3.100","article-title":"Insulin resistance in obese children and adolescents: HOMA-IR cut-off levels in the prepubertal and pubertal periods","volume":"2","author":"Kendirci","year":"2010","journal-title":"J. Clin. Res. Pediatr. Endocrinol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Fr\u00fchbeck, G., Catal\u00e1n, V., Rodr\u00edguez, A., Ram\u00edrez, B., Becerril, S., Salvador, J., Colina, I., and G\u00f3mez-Ambrosi, J. (2019). Adiponectin-leptin ratio is a functional biomarker of adipose tissue inflammation. Nutrients, 11.","DOI":"10.3390\/nu11020454"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/oby.2007.531","article-title":"Higher protein intake preserves lean mass and satiety with weight loss in pre-obese and obese women","volume":"15","author":"Leidy","year":"2007","journal-title":"Obesity"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Samudio-Cruz, M.A., Luna-Angulo, A., Cabrera-Ruiz, E., Landa-Solis, C., Rangel-L\u00f3pez, E., Carrilo-Mora, P., R\u00edos-Mart\u00ednez, J., Toledo-P\u00e9rez, R., Paniagua-P\u00e9rez, R., and Mart\u00ednez-Canseco, C.J. (2025). The bacterial composition of the gut microbiota of Mexicans with overweight and obesity: A systematic review. Microorganisms, 13.","DOI":"10.3390\/microorganisms13081727"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ily\u00e9s, T., Silaghi, C.N., and Cr\u00e2ciun, A.M. (2022). Diet-related changes of shorth-chain fatty acids in blood and feces in obesity and metabolic syndrome. Biology, 11.","DOI":"10.3390\/biology11111556"},{"key":"ref_19","first-page":"e13807","article-title":"Leptin is a potential biomarker of childhood obesity and an indicator of the effectivenss of weight-loss interventions","volume":"25","author":"Mahmood","year":"2023","journal-title":"Obes. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1016\/j.clnu.2022.06.007","article-title":"Gut microbiota is associated with metabolic health in children with obesity","volume":"41","author":"Alcazar","year":"2022","journal-title":"Clin. Nutr."},{"key":"ref_21","first-page":"612","article-title":"Comparison of eating habits and gut microbiota of preschool children with obesity","volume":"4","year":"2023","journal-title":"Explor. Med."},{"key":"ref_22","first-page":"11","article-title":"High relative abundance of firmicutes and increased TNF-a levels correlate with obesity in children","volume":"605","year":"2018","journal-title":"Salud Publica Mex."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5160","DOI":"10.1038\/s41467-023-40874-x","article-title":"Gut microbiota and fecal short chain fatty acids differ with adiposity and country of origin. The METS-mcirobiome study","volume":"14","author":"Maseng","year":"2023","journal-title":"Nat. Comm."},{"key":"ref_24","first-page":"180","article-title":"Low dietary fiber intake increases Collinsella abundance in the gut microbiota of overweight and obese pregnant women","volume":"9","author":"Barret","year":"2018","journal-title":"Gut Microbes"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2298246","DOI":"10.1080\/19490976.2023.2298246","article-title":"Collinsella aerofacines as a predictive marker of response to probitic treatment in non-constipated irritable bowel s\u00edndrome","volume":"16","author":"Gargari","year":"2024","journal-title":"Gut Microbes"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00203-023-03750-2","article-title":"Anti-obesity activity of human gut microbiota Bacteroides stercoris KGMB02265","volume":"206","author":"Ryu","year":"2024","journal-title":"Arch. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.jad.2024.04.050","article-title":"Coprococcus eutactus screened from healthy adolescents attenuates chronic restraint stress-induced depression-like changes in adolescent mice: Potential roles in the microbiome and neurotransmitter modulation","volume":"356","author":"Xu","year":"2024","journal-title":"J. Affect Disord."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e0054425","DOI":"10.1128\/msystems.00544-25","article-title":"A three-country analysis of the gut microbiome indicates taxon associations with diet vary by taxon resolution and population","volume":"10","author":"Khatib","year":"2025","journal-title":"mSystems"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1002\/fft2.417","article-title":"Prevotella copri\u2014A potential next-generation probiotic","volume":"5","author":"Zhang","year":"2024","journal-title":"Food Front."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e59","DOI":"10.1002\/imo2.59","article-title":"Multi-omics reveals different signatures of obesity-prone and obesity-resistant mice","volume":"2","author":"Wang","year":"2024","journal-title":"iMetaOmics"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Chandra, W., Jiang, H., and Liu, S.-J. (2024). The ambiguous correlation of Blautia with obesity: A systematic review. Microorganisms, 12.","DOI":"10.3390\/microorganisms12091768"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"16009","DOI":"10.1038\/npjbiofilms.2016.9","article-title":"Oral treatment with Eubacterium hallii improves insulin sensitivity in db\/db mice","volume":"2","author":"Udayappan","year":"2016","journal-title":"npj Biofilms Microbiomes"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e00094-18","DOI":"10.1128\/msystems.00094-18","article-title":"Butyrate producers as potential next-generation probiotics: Safety assessment of the administrationof Butyricicoccus pullicaecorum to healthy volunteers","volume":"3","author":"Boesmans","year":"2018","journal-title":"mSystems"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Nie, K., Ma, K., Luo, W., Shen, Z., Yang, Z., Xiao, M., Tong, T., Yang, Y., and Wang, X. (2021). Roseburia intestinalis: A beneficial gut organism from the discoveries in genus and species. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.757718"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.anaerobe.2018.10.009","article-title":"The gut microbiome of Mexican children affected by obesity","volume":"55","author":"Murugesan","year":"2019","journal-title":"Anaerobe"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Lakshmanan, A.P., Al Zaidan, S., Bangarusamy, D.K., Al-Shamari, S., Elhag, W., and Terranegra, A. (2022). Increased relative abundance of Ruminoccocus is associated with reduced cardiovascular risk in an obese population. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.849005"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.2337\/db19-1087","article-title":"Transketolase deficiency in adipose tissues protects mice from diet-induced obesity by promoting lipolysis","volume":"69","author":"Tian","year":"2020","journal-title":"Diabetes"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1021\/acsptsci.3c00028","article-title":"Distinct metabolomics profiling of serum samples from high-fat-diet-induced insulin-resistant mice","volume":"6","author":"Sharma","year":"2023","journal-title":"ACS Pharmacol. Transl. Sci."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/11\/1690\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T01:10:05Z","timestamp":1781053805000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/11\/1690"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,26]]},"references-count":38,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["nu18111690"],"URL":"https:\/\/doi.org\/10.3390\/nu18111690","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,26]]}}}