{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:38:01Z","timestamp":1788964681522,"version":"build-2803163510"},"reference-count":70,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015498","name":"Ministry for Culture and Innovation from the Source of the National Research, Development and Innovation Fund","doi-asserted-by":"publisher","award":["EK\u00d6P-24-3-SZTE-213"],"award-info":[{"award-number":["EK\u00d6P-24-3-SZTE-213"]}],"id":[{"id":"10.13039\/501100015498","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015763","name":"University of Szeged","doi-asserted-by":"crossref","award":["8685"],"award-info":[{"award-number":["8685"]}],"id":[{"id":"10.13039\/501100015763","id-type":"DOI","asserted-by":"crossref"}]},{"award":["8685"],"award-info":[{"award-number":["8685"]}],"id":[{"id":"https:\/\/ror.org\/01pnej532","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Objectives: Obesity and the associated metabolic dysfunction influence fertility performance at molecular levels and ABC transporters are considered as potential molecular factors affecting fertility both in the testis and sperm; therefore, we aimed to examine the effect of a short-term diet-induced obesity on testicular and spermatic ABC transporters in a rat model focusing on the expressions of P-glycoprotein (P-gp, Abcb1) and breast cancer resistance protein (BCRP, Abcg2). The testicular androgen state involving aromatase enzyme (Cyp19a1), androgen receptor (Ar), and testosterone levels were also evaluated. Methods: Obesity was induced in male Sprague Dawley rats by feeding a high-fat, high-sugar diet (HFHSD) for 10 weeks, and metabolic status was evaluated using a glucose tolerance test. The weight and size of reproductive organs were measured, and Abcb1a\/1b, Abcg2, Cyp19a1, and Ar expression in testes or sperm was determined by RT-PCR and Western blotting. At the same time, testosterone levels were measured by ELISA. Results: HFHSD successfully induced higher weight gain with glucose intolerance and reduced reproductive organ size. In obese rats, testicular Abcb1a and Abcb1b mRNA and P-gp protein expression were significantly higher, whereas testicular Abcg2 mRNA levels decreased. Spermatic Abcb1a, Abcb1b and Abcg2 mRNA expression also reduced in obesity. Neither testicular testosterone concentration nor Cyp19a1 and Ar mRNA expression levels changed after the 10-week obesogenic diet compared with controls. Conclusions: Overall, our study revealed infertility-related ABC transporter changes in obese male rats, suggesting that these alterations may predispose obese males to fertility impairments, even before the obesity-induced androgen dysregulation.<\/jats:p>","DOI":"10.3390\/nu18111829","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:10:42Z","timestamp":1780668642000},"page":"1829","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Obesity Triggers Dysregulation of Essential ABC Transporters in Rat Testis and Sperm"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1408-3286","authenticated-orcid":false,"given":"P\u00e9ter","family":"Szatm\u00e1ri","sequence":"first","affiliation":[{"name":"Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s Street 6, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2746-5372","authenticated-orcid":false,"given":"Kata Kira","family":"Kem\u00e9ny","sequence":"additional","affiliation":[{"name":"Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s Street 6, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienn","family":"Seres-Bokor","sequence":"additional","affiliation":[{"name":"Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s Street 6, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5219-8491","authenticated-orcid":false,"given":"Eszter","family":"Ducza","sequence":"additional","affiliation":[{"name":"Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s Street 6, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"ref_1","unstructured":"Lobstein, T., Powis, J., and Jackson-Leach, R. (2024). World Obesity Atlas 2024, World Obesity Federation. [6th ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2548","DOI":"10.1210\/jc.2004-0395","article-title":"Adipose Tissue as an Endocrine Organ","volume":"89","author":"Kershaw","year":"2004","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"155144","DOI":"10.1016\/j.cyto.2020.155144","article-title":"Adipocytokines: Are They the Theory of Everything?","volume":"133","author":"Maximus","year":"2020","journal-title":"Cytokine"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Singh, R., and Singh, K. (2017). Obesity, Spermatogenesis, and Male Infertility. Male Infertility: Understanding, Causes and Treatment, Springer.","DOI":"10.1007\/978-981-10-4017-7"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"George, B.T., Jhancy, M., Dube, R., Kar, S.S., and Annamma, L.M. (2023). The Molecular Basis of Male Infertility in Obesity: A Literature Review. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25010179"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1097\/01.ede.0000229953.76862.e5","article-title":"Reduced Fertility Among Overweight and Obese Men","volume":"17","author":"Sandler","year":"2006","journal-title":"Epidemiology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"79","DOI":"10.5173\/ceju.2015.01.435","article-title":"Mechanisms Linking Obesity to Male Infertility","volume":"68","author":"Katib","year":"2015","journal-title":"Cent. Eur. J. Urol."},{"key":"ref_8","first-page":"e30770","article-title":"Obesity: A Doorway to a Molecular Path Leading to Infertility","volume":"14","author":"Ahmad","year":"2022","journal-title":"Cureus"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"102393","DOI":"10.1016\/j.bpobgyn.2023.102393","article-title":"Obesity and Male Infertility","volume":"90","author":"Ameratunga","year":"2023","journal-title":"Best Pract. Res. Clin. Obs. Gynaecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s43043-022-00099-2","article-title":"Obesity and Male Infertility: Multifaceted Reproductive Disruption","volume":"27","author":"Chaudhuri","year":"2022","journal-title":"Middle East Fertil. Soc. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1210\/er.2014-1101","article-title":"The Mammalian Blood-Testis Barrier: Its Biology and Regulation","volume":"36","author":"Mruk","year":"2015","journal-title":"Endocr. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/aja.2013.24","article-title":"Breast Cancer Resistance Protein (Bcrp) and the Testis\u2014An Unexpected Turn of Events","volume":"15","author":"Qian","year":"2013","journal-title":"Asian J. Androl."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Szatm\u00e1ri, P., and Ducza, E. (2023). Changes in Expression and Function of Placental and Intestinal P-Gp and BCRP Transporters during Pregnancy. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241713089"},{"key":"ref_14","first-page":"164","article-title":"ATP-Binding Cassette Transporters in Reproduction: A New Frontier","volume":"22","author":"Bloise","year":"2015","journal-title":"Hum. Reprod. Update"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s10020-023-00776-6","article-title":"Small Noncoding RNAs and Sperm Nuclear Basic Proteins Reflect the Environmental Impact on Germ Cells","volume":"30","author":"Ferrero","year":"2024","journal-title":"Mol. Med."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Szatm\u00e1ri, P., Kem\u00e9ny, K.K., Sur\u00e1nyi, A., Rachamim, Y., and Ducza, E. (2025). Alterations in P-Glycoprotein Expression in the Placenta of Obese Rats and Humans. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26146976"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.bbrc.2019.09.067","article-title":"Testosterone and Androstenedione Are Endogenous Substrates of P-Glycoprotein","volume":"520","author":"Yano","year":"2019","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.xphs.2023.10.034","article-title":"Impact of P-Glycoprotein-Mediated Drug-Endogenous Substrate Interactions on Androgen and Blood-Brain Barrier Permeability","volume":"113","author":"Sunakawa","year":"2024","journal-title":"J. Pharm. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Liu, Y., Chen, Y., Wei, B., Li, H., Peng, Y., and Luo, Z. (2024). Impacts of ABCG2 Loss of Function Variant (p. Gln141Lys, c.421 C > A, Rs2231142) on Lipid Levels and Statin Efficiency: A Systematic Review and Meta-Analysis. BMC Cardiovasc. Disord., 24.","DOI":"10.1186\/s12872-024-03821-2"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1124\/mol.111.075143","article-title":"Characterization of the Role of ABCG2 as a Bile Acid Transporter in Liver and Placenta","volume":"81","author":"Blazquez","year":"2012","journal-title":"Mol. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3773","DOI":"10.1007\/s10528-024-10902-6","article-title":"Association of Functional Genetic Variations in Uric Acid Transporters with the Risk of Idiopathic Male Infertility: A Genetic Association Study and Bioinformatic Analysis","volume":"63","author":"Karimian","year":"2025","journal-title":"Biochem. Genet."},{"key":"ref_22","first-page":"6412","article-title":"Drug Transport across the Blood-Testis Barrier","volume":"14","author":"Meng","year":"2022","journal-title":"Am. J. Transl. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0092-8674(94)90212-7","article-title":"Disruption of the Mouse Mdr1a P-Glycoprotein Gene Leads to a Deficiency in the Blood-Brain Barrier and to Increased Sensitivity to Drugs","volume":"77","author":"Schinkel","year":"1994","journal-title":"Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1007\/s11095-014-1609-7","article-title":"Brain and Testis Accumulation of Regorafenib Is Restricted by Breast Cancer Resistance Protein (BCRP\/ABCG2) and P-Glycoprotein (P-GP\/ABCB1)","volume":"32","author":"Kort","year":"2015","journal-title":"Pharm. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1039\/C5TX00058K","article-title":"The Role of Breast Cancer Resistance Protein (Bcrp\/Abcg2) in Triptolide-Induced Testis Toxicity","volume":"4","author":"Li","year":"2015","journal-title":"Toxicol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.etap.2008.03.011","article-title":"Effect of Ivermectin on Male Fertility and Its Interaction with P-Glycoprotein Inhibitor (Verapamil) in Rats","volume":"26","year":"2008","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_27","unstructured":"Dias, M.S. (2022). Spermatogenesis Protection by P-Glycoprotein Modulation, University of Porto."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.bbrc.2008.11.058","article-title":"ABCG2 Is Expressed in Late Spermatogenesis and Is Associated with the Acrosome","volume":"378","author":"Scharenberg","year":"2009","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1093\/toxsci\/kfq318","article-title":"Regulation and Characterization of the ATP-Binding Cassette Transporter-B1 in the Epididymis and Epididymal Spermatozoa of the Rat","volume":"119","author":"Jones","year":"2011","journal-title":"Toxicol. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"653","DOI":"10.3906\/vet-1404-55","article-title":"Cholesterol Efflux from Sperm: Approaches and Applications","volume":"38","author":"Naseer","year":"2014","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Xue, S.-H., Xu, B.-B., Yan, X.-C., Zhang, J.-X., and Su, R. (2025). Sperm Membrane Stability: In-Depth Analysis from Structural Basis to Functional Regulation. Vet. Sci., 12.","DOI":"10.3390\/vetsci12070658"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/S1734-1140(09)70121-5","article-title":"Association of the MDR1 (ABCB1) Gene 3435C> T Polymorphism with Male Infertility","volume":"61","author":"Stefankiewicz","year":"2009","journal-title":"Pharmacol. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1080\/17425255.2020.1701653","article-title":"Modulation of Expression\/Function of Intestinal P-Glycoprotein under Disease States","volume":"16","author":"Murakami","year":"2020","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.bcp.2019.05.002","article-title":"Diet-Induced Obese Alters the Expression and Function of Hepatic Drug-Metabolizing Enzymes and Transporters in Rats","volume":"164","author":"Zhang","year":"2019","journal-title":"Biochem. Pharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1007\/s43440-024-00665-7","article-title":"Obesity-Related Drug Transporter Expression Alterations in Human Liver and Kidneys","volume":"76","author":"Sheng","year":"2024","journal-title":"Pharmacol. Rep."},{"key":"ref_36","first-page":"370","article-title":"Influence of Sex Hormones on the ABCG2 Transport Protein in Caco-2 Cells","volume":"40","author":"Slepnev","year":"2023","journal-title":"Biol. Membr."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"G\u00e1sp\u00e1r, R., Hajagos-T\u00f3th, J., Schaffer, A., Kothencz, A., Siska-Szab\u00f3, L., Ducza, E., Cs\u00e1nyi, A., T\u00e1bi, T., Bagam\u00e9ry, F., and Sz\u00f6k\u0151, \u00c9. (2022). High Fat High Sucrose Diet Modifies Uterine Contractility and Cervical Resistance in Pregnant Rats: The Roles of Sex Hormones, Adipokines and Cytokines. Life, 12.","DOI":"10.3390\/life12060794"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e42435","DOI":"10.1016\/j.heliyon.2025.e42435","article-title":"Investigation of Calonysterone and 20-Hydroxyecdysone Effects in High-Fat, High-Sugar Diet-Induced Obesity Rat Model","volume":"11","author":"Osman","year":"2025","journal-title":"Heliyon"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1095\/biolreprod.108.071951","article-title":"Estrogen-Induced Developmental Disorders of the Rat Penis Involve Both Estrogen Receptor (ESR)- and Androgen Receptor (AR)-Mediated Pathways1","volume":"81","author":"Goyal","year":"2009","journal-title":"Biol. Reprod."},{"key":"ref_40","first-page":"384","article-title":"Testicular Morphology of Male Rats Exposed to Phaleria Macrocarpa (Mahkota Dewa) Aqueous Extract","volume":"17","author":"Parhizkar","year":"2014","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1006\/abio.1987.9999","article-title":"Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate\u2013Phenol\u2013Chloroform Extraction","volume":"162","author":"Chomzynski","year":"1987","journal-title":"Anal. Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Jahangir, M., Nazari, M., Babakhanzadeh, E., and Manshadi, S.D. (2024). Where Do Obesity and Male Infertility Collide?. BMC Med. Genom., 17.","DOI":"10.1186\/s12920-024-01897-5"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/ijo.2013.159","article-title":"The Snacking Rat as Model of Human Obesity: Effects of a Free-Choice High-Fat High-Sugar Diet on Meal Patterns","volume":"38","author":"Luijendijk","year":"2014","journal-title":"Int. J. Obes."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Matias, A.M., Estevam, W.M., Coelho, P.M., Haese, D., Kobi, J.B.B.S., Lima-Leopoldo, A.P., and Leopoldo, A.S. (2018). Differential Effects of High Sugar, High Lard or a Combination of Both on Nutritional, Hormonal and Cardiovascular Metabolic Profiles of Rodents. Nutrients, 10.","DOI":"10.3390\/nu10081071"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"215","DOI":"10.3325\/cmj.2021.62.215","article-title":"Sex Differences in Oxidative Stress Level and Antioxidative Enzymes Expression and Activity in Obese Pre-Diabetic Elderly Rats Treated with Metformin or Liraglutide","volume":"62","author":"Heffer","year":"2021","journal-title":"Croat. Med. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/1477-7827-9-32","article-title":"Diet-Induced Obesity in Rats Leads to a Decrease in Sperm Motility","volume":"9","author":"Fernandez","year":"2011","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Falvo, S., Minucci, S., Santillo, A., Senese, R., Chieffi Baccari, G., and Venditti, M. (2023). A Short-Term High-Fat Diet Alters Rat Testicular Activity and Blood-Testis Barrier Integrity through the SIRT1\/NRF2\/MAPKs Signaling Pathways. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1274035"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e171141049830","DOI":"10.33448\/rsd-v14i10.49830","article-title":"Negative Effects of Sucrose Ingestion in Testis Weight and Epididymal Fat of Adult Wistar Rats","volume":"14","author":"Silva","year":"2025","journal-title":"Res. Soc. Dev."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.1210\/clinem\/dgab535","article-title":"Obesity Is Strongly Associated with Low Testosterone and Reduced Penis Growth During Development","volume":"106","author":"Mancini","year":"2021","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"189","DOI":"10.4111\/icu.20230287","article-title":"Pediatric Obesity and Development of the Penis and Testis","volume":"65","author":"Lee","year":"2024","journal-title":"Investig. Clin. Urol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.tips.2010.11.007","article-title":"Emerging Role for Drug Transporters at the Blood\u2013Testis Barrier","volume":"32","author":"Mruk","year":"2011","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e3038","DOI":"10.1038\/cddis.2017.435","article-title":"Abcb1a and Abcb1b Genes Function Differentially in Blood\u2013Testis Barrier Dynamics in the Rat","volume":"8","author":"Su","year":"2017","journal-title":"Cell Death Dis."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Jia, Y.-F., Feng, Q., Ge, Z.-Y., Guo, Y., Zhou, F., Zhang, K.-S., Wang, X.-W., Lu, W.-H., Liang, X.-W., and Gu, Y.-Q. (2018). Obesity Impairs Male Fertility through Long-Term Effects on Spermatogenesis. BMC Urol., 18.","DOI":"10.1186\/s12894-018-0360-5"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Pini, T., Raubenheimer, D., Simpson, S.J., and Crean, A.J. (2021). Obesity and Male Reproduction; Placing the Western Diet in Context. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.622292"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Ruiz-Valderrama, L., Mendoza-S\u00e1nchez, J.E., Rodr\u00edguez-Tob\u00f3n, E., Arrieta-Cruz, I., Gonz\u00e1lez-M\u00e1rquez, H., Salame-M\u00e9ndez, P.A., Tarrag\u00f3-Castellanos, R., Cort\u00e9s-Barberena, E., Rodr\u00edguez-Tob\u00f3n, A., and Arenas-R\u00edos, E. (2025). High-Fat Diets Disturb Rat Epididymal Sperm Maturation. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26051850"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1016\/j.fertnstert.2014.07.1248","article-title":"Acrosome Reaction Is Impaired in Spermatozoa of Obese Men: A Preliminary Study","volume":"102","author":"Samavat","year":"2014","journal-title":"Fertil. Steril."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/978-3-030-77779-1_9","article-title":"Androgen Actions in the Testis and the Regulation of Spermatogenesis","volume":"Volume 1381","author":"Cheng","year":"2021","journal-title":"Molecular Mechanisms in Spermatogenesis"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1590\/S1677-5538.IBJU.2014.0244","article-title":"Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult Rats","volume":"41","author":"Furriel","year":"2015","journal-title":"Int. Braz. J. Urol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Donner, D.G., Elliott, G.E., Beck, B.R., Bulmer, A.C., and Du Toit, E.F. (2015). Impact of Diet-Induced Obesity and Testosterone Deficiency on the Cardiovascular System: A Novel Rodent Model Representative of Males with Testosterone-Deficient Metabolic Syndrome (TDMetS). PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0138019"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1177\/0192623318803557","article-title":"The Effect of Diet-Induced Obesity on Toxicological Parameters in the Polygenic Sprague-Dawley Rat Model","volume":"46","author":"Rojas","year":"2018","journal-title":"Toxicol. Pathol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e23656","DOI":"10.1096\/fj.202301864RR","article-title":"Susceptibility to Recombinant SARS-CoV-2 Spike Protein Entry in the Lungs of High-Fat Diet-Induced Obese Mice","volume":"38","author":"Gotoh","year":"2024","journal-title":"FASEB J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1016\/0959-8049(95)00130-B","article-title":"Multidrug Resistance and the Role of P-Glycoprotein Knockout Mice","volume":"31","author":"Schinkel","year":"1995","journal-title":"Eur. J. Cancer"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4028","DOI":"10.1073\/pnas.94.8.4028","article-title":"Normal Viability and Altered Pharmacokinetics in Mice Lacking Mdr1-Type (Drug-Transporting) P-Glycoproteins","volume":"94","author":"Schinkel","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1124\/mol.107.034751","article-title":"Effect of Breast Cancer Resistance Protein (Bcrp\/Abcg2) on the Disposition of Phytoestrogens","volume":"72","author":"Enokizono","year":"2007","journal-title":"Mol. Pharmacol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1124\/dmd.107.019257","article-title":"Quantitative Investigation of the Role of Breast Cancer Resistance Protein (Bcrp\/Abcg2) in Limiting Brain and Testis Penetration of Xenobiotic Compounds","volume":"36","author":"Enokizono","year":"2008","journal-title":"Drug Metab. Dispos."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1158\/1535-7163.MCT-20-0705","article-title":"ABCB1 and ABCG2 Restrict Brain and Testis Accumulation and, Alongside CYP3A, Limit Oral Availability of the Novel TRK Inhibitor Selitrectinib","volume":"20","author":"Li","year":"2021","journal-title":"Mol. Cancer Ther."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Kapoor, A., Iqbal, M., Petropoulos, S., Ho, H.L., Gibb, W., and Matthews, S.G. (2013). Effects of Sertraline and Fluoxetine on P-Glycoprotein at Barrier Sites: In Vivo and In Vitro Approaches. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0056525"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"e70027","DOI":"10.1111\/cts.70027","article-title":"Is There a Possibility That P-Glycoprotein Reduces Reproductive Toxicity in Males but Breast Cancer Resistance Protein Does Not?","volume":"17","author":"Yu","year":"2024","journal-title":"Clin. Transl. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1093\/jac\/dkw046","article-title":"Antiretroviral Drug Transporters and Metabolic Enzymes in Human Testicular Tissue: Potential Contribution to HIV-1 Sanctuary Site","volume":"71","author":"Huang","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"104791","DOI":"10.1016\/j.jddst.2023.104791","article-title":"Importance of P-Gp Inhibitors and Nanoengineered Approaches for Effective Delivery of Anti-Retroviral Drugs across Barriers in HIV Management","volume":"87","author":"Aashish","year":"2023","journal-title":"J. Drug Deliv. Sci. 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