{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:50:51Z","timestamp":1792479051860,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Xingdian Industrial Innovation Talent Project","award":["yfgrc202536"],"award-info":[{"award-number":["yfgrc202536"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: In this study, a cultivated mushroom (Buchwaldoboletus sp.) polysaccharide (BSP60) was prepared. Methods: The protective effect of BSP60 on ulcerative colitis (UC) was evaluated using a dextran sulphate sodium (DSS)-induced murine model of UC. Results: The results showed that BSP60 significantly alleviated colonic inflammation and oxidant stress in DSS-treated mice. BSP60 reduced the overproduction of TNF-\u03b1, IL-1\u03b2, and IL-6; lowered the accumulation of MDA and LPS in serum and colon; and increased the activities of antioxidant enzymes SOD and GSH-Px in colonic tissues. Histological observations revealed that BSP60 significantly alleviated DSS-induced colonic tissue damage in mice by reducing inflammatory cell infiltration and mucosal injury. Immunofluorescence staining revealed that BSP60 increased the expression of intestinal barrier-associated proteins ZO-1, occludin, and claudin-1 in DSS-induced UC mice, thereby alleviating inflammation. Analysis of 16S rRNA gene sequencing indicated that BSP60 maintained gut microbiota homeostasis in UC mice and inhibited the excessive growth of inflammation-associated microorganisms. BSP60 also enhanced the production of short-chain fatty acids in the mice feces. The protective effect of BSP60 on UC was dose dependent. Conclusions: BSP60 exerts anti-UC effects, providing a scientific basis for its application as a functional food ingredient to improve intestinal health.<\/jats:p>","DOI":"10.3390\/nu18142225","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:40:09Z","timestamp":1783525209000},"page":"2225","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of a Polysaccharide from Cultivated Buchwaldoboletus sp. on DSS-Induced Ulcerative Colitis in Mice"],"prefix":"10.3390","volume":"18","author":[{"given":"Liyuan","family":"Ba","sequence":"first","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Tu","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yirong","family":"Mao","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihui","family":"Li","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Sun","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"145074","DOI":"10.1016\/j.ijbiomac.2025.145074","article-title":"Structural elucidation of polysaccharide from fresh Areca catechu L. and its mitigation effect on DSS-induced colitis in mice","volume":"318","author":"Wang","year":"2025","journal-title":"Int. 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