{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T10:04:10Z","timestamp":1784282650317,"version":"3.55.0"},"reference-count":22,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Antidiabetic Food Centre","award":["2013\/46"],"award-info":[{"award-number":["2013\/46"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background\/Objectives: Non-caloric sweeteners are increasingly used as alternatives to sugar to reduce energy intake, yet their metabolic effects remain controversial. This study aimed to evaluate the effects of sucrose, saccharin, and steviol glycosides on glucose regulation and cardiometabolic risk markers in healthy adults. Methods: In a randomized, double-blind, crossover trial, 39 healthy, normal-weight adults consumed beverages containing sucrose (66 g\/day), saccharin (220 mg\/day), or steviol glycosides (220 mg\/day) for 14 days, with washout periods between interventions. Metabolic outcomes were assessed at baseline and after each intervention under fasting conditions and during a 2 h postprandial test. Primary outcomes were glucose and insulin responses; secondary outcomes included gut hormones, lipids, inflammatory markers, and subjective appetite. Results: Compared with baseline, fasting glucose increased after sucrose and saccharin, and fasting insulin increased after stevia (p &lt; 0.01). All interventions increased postprandial insulin responses and reduced indices of insulin sensitivity (p &lt; 0.05). Fasting PYY and GLP-2 increased following all treatments (p &lt; 0.001), without differences between sweeteners. Triglycerides were higher after sucrose than saccharin (p &lt; 0.05), while no differences were seen for cholesterol, apolipoproteins, or CRP. Appetite ratings were unchanged, with a trend (p = 0.053) towards a reduced desire to eat after stevia in the late postprandial phase. Conclusions: Short-term intake of sucrose and non-caloric sweeteners resulted in broadly similar metabolic responses, with no significant differences in glucose regulation. However, triglyceride concentrations were higher following sucrose than saccharin, whereas no consistent differences were observed across the remaining outcomes. Observed deviations from baseline should be interpreted with caution. Further long-term studies in diverse populations are warranted.<\/jats:p>","DOI":"10.3390\/nu18142337","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:55:59Z","timestamp":1784249759000},"page":"2337","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Short-Term (14-Day) Intake of Sucrose and Non-Caloric Sweeteners on Glucose Regulation, Blood Lipids, Gut Hormones, Inflammation Markers, and Appetite in Healthy Adults: A Randomized Controlled Trial"],"prefix":"10.3390","volume":"18","author":[{"given":"Anne","family":"Nilsson","sequence":"first","affiliation":[{"name":"Division Food and Pharma, Department of Process and Life Science Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/s41574-021-00627-6","article-title":"The role of sugar-sweetened beverages in the global epidemics of obesity and chronic diseases","volume":"18","author":"Malik","year":"2022","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.3945\/ajcn.110.007922","article-title":"Sugar-sweetened and artificially sweetened beverage consumption and risk of type 2 diabetes in men","volume":"93","author":"Malik","year":"2011","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1017\/S1368980021001580","article-title":"Trends and patterns in sugar-sweetened beverage consumption among children and adults by race and\/or ethnicity, 2003-2018","volume":"24","author":"Dai","year":"2021","journal-title":"Public Health Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/s41430-018-0170-6","article-title":"Glycemic impact of non-nutritive sweeteners: A systematic review and meta-analysis of randomized controlled trials","volume":"72","author":"Nichol","year":"2018","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1038\/ijo.2015.177","article-title":"Does low-energy sweetener consumption affect energy intake and body weight? A systematic review, including meta-analyses, of the evidence from human and animal studies","volume":"40","author":"Rogers","year":"2016","journal-title":"Int. J. Obes."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.physbeh.2009.12.021","article-title":"High-intensity sweeteners and energy balance","volume":"100","author":"Swithers","year":"2010","journal-title":"Physiol. Behav."},{"key":"ref_7","first-page":"101","article-title":"Gain weight by \u201cgoing diet?\u201d Artificial sweeteners and the neurobiology of sugar cravings: Neuroscience 2010","volume":"83","author":"Yang","year":"2010","journal-title":"Yale J. Biol. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"688","DOI":"10.2337\/dc08-1799","article-title":"Diet soda intake and risk of incident metabolic syndrome and type 2 diabetes in the Multi-Ethnic Study of Atherosclerosis (MESA)","volume":"32","author":"Nettleton","year":"2009","journal-title":"Diabetes Care"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nature13793","article-title":"Artificial sweeteners induce glucose intolerance by altering the gut microbiota","volume":"514","author":"Suez","year":"2014","journal-title":"Nature"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"k4718","DOI":"10.1136\/bmj.k4718","article-title":"Association between intake of non-sugar sweeteners and health outcomes: Systematic review and meta-analyses of randomised and non-randomised controlled trials and observational studies","volume":"364","author":"Toews","year":"2019","journal-title":"BMJ"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1093\/ajcn\/nqy381","article-title":"A randomized controlled trial contrasting the effects of 4 low-calorie sweeteners and sucrose on body weight in adults with overweight or obesity","volume":"109","author":"Higgins","year":"2019","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Leibowitz, A., Bier, A., Gilboa, M., Peleg, E., Barshack, I., and Grossman, E. (2018). Saccharin Increases Fasting Blood Glucose but Not Liver Insulin Resistance in Comparison to a High Fructose-Fed Rat Model. Nutrients, 10.","DOI":"10.3390\/nu10030341"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3908","DOI":"10.1039\/D3FO04695H","article-title":"Effect of steviol glycosides as natural sweeteners on glucose metabolism in adult participants","volume":"15","author":"Bai","year":"2024","journal-title":"Food Funct."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1097\/MOL.0000000000000034","article-title":"Non-nutritive sweeteners: Evidence for benefit vs. risk","volume":"25","author":"Gardner","year":"2014","journal-title":"Curr. Opin. Lipidol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"21106","DOI":"10.1038\/s41598-025-00612-3","article-title":"The effects of non-nutritive sweeteners on lipid profile in adults: A systematic review and meta-analysis of randomized controlled clinical trials","volume":"15","author":"Najafi","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_16","unstructured":"World Health Organization (1999). Definition, Diagnosis and Classification of Diabetes Mellitus and its Complications; Report of a WHO Consultation; Part 1: Diagnosis and Classification of Diabetes Mellitus, WHO."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.fct.2017.06.046","article-title":"Sweeteners as food additives in the XXI century: A review of what is known, and what is to come","volume":"107","author":"Carocho","year":"2017","journal-title":"Food Chem. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1093\/clinchem\/18.6.499","article-title":"Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge","volume":"18","author":"Friedewald","year":"1972","journal-title":"Clin. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.2337\/diacare.22.9.1462","article-title":"Insulin sensitivity indices obtained from oral glucose tolerance testing: Comparison with the euglycemic insulin clamp","volume":"22","author":"Matsuda","year":"1999","journal-title":"Diabetes Care"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.2337\/diacare.27.6.1487","article-title":"Use and Abuse of HOMA Modeling","volume":"27","author":"Wallace","year":"2004","journal-title":"Diabetes Care"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1017\/S0007114515002524","article-title":"Increased gut hormones and insulin sensitivity index following a 3-d intervention with a barley kernel-based product: A randomised cross-over study in healthy middle-aged subjects","volume":"114","author":"Nilsson","year":"2015","journal-title":"Br. J. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1161\/01.ATV.0000021150.63480.CD","article-title":"Circulating oxidized LDL is associated with subclinical atherosclerosis development and inflammatory cytokines (AIR Study)","volume":"22","author":"Hulthe","year":"2002","journal-title":"Arterioscler. Thromb. Vasc. Biol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/14\/2337\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T09:15:52Z","timestamp":1784279752000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/14\/2337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,16]]},"references-count":22,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["nu18142337"],"URL":"https:\/\/doi.org\/10.3390\/nu18142337","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,16]]}}}