{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:34:50Z","timestamp":1785418490228,"version":"3.56.0"},"reference-count":37,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:00:00Z","timestamp":1784332800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Joint TCM Science & Technology Projects of National Demonstration Zones for Comprehensive TCM Reform","award":["GZY-KJS-ZJ-2026-012"],"award-info":[{"award-number":["GZY-KJS-ZJ-2026-012"]}]},{"name":"Zhejiang Province San-Nong-Jiu-Fang Science and Technology Cooperation Project","award":["2026SNJF068"],"award-info":[{"award-number":["2026SNJF068"]}]},{"DOI":"10.13039\/501100004863","name":"Zhejiang Chinese Medical University","doi-asserted-by":"crossref","award":["2025JKZDZC06"],"award-info":[{"award-number":["2025JKZDZC06"]}],"id":[{"id":"10.13039\/501100004863","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004863","name":"Zhejiang Chinese Medical University","doi-asserted-by":"crossref","award":["2025JKZKTS32"],"award-info":[{"award-number":["2025JKZKTS32"]}],"id":[{"id":"10.13039\/501100004863","id-type":"DOI","asserted-by":"crossref"}]},{"award":["2025JKZDZC06"],"award-info":[{"award-number":["2025JKZDZC06"]}],"id":[{"id":"https:\/\/ror.org\/04epb4p87","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2025JKZKTS32"],"award-info":[{"award-number":["2025JKZKTS32"]}],"id":[{"id":"https:\/\/ror.org\/04epb4p87","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074085"],"award-info":[{"award-number":["82074085"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["82074085"],"award-info":[{"award-number":["82074085"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005150","name":"Academy of Chinese Medical Sciences","doi-asserted-by":"crossref","award":["2025J04"],"award-info":[{"award-number":["2025J04"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background\/Objectives: Gouty arthritis (GA) is an inflammatory disease caused by increased purine metabolism. The limitations of current anti-GA therapies remain a major challenge. Astilbin, the main flavonoid in Smilax glabra Roxb., was found to exert potential anti-GA effects in our previous study. Methods: In this study, a mouse model of monosodium urate (MSU)-induced arthritis and an inflammatory model using mouse bone marrow-derived macrophages (BMDMs) were established. Results: Our data showed that astilbin reduced MSU-induced joint swelling and inflammatory infiltration in mice, restored lipopolysaccharide (LPS)\/MSU-induced reductions in cell viability, and inhibited the expression levels of inflammatory factors IL-1\u03b2, IL-6 and TNF-\u03b1. Further studies showed that astilbin significantly reduced MSU-induced increases in NLRP3 and P-p65 protein levels, as well as the expression of ASC, P-IKK\u03b1, P-I\u03baB\u03b1, and cleaved-caspase-1. Conclusions: This study suggests that astilbin may be a promising natural product for the treatment of GA by inhibiting the activation of the NLRP3 inflammasome and NF-\u03baB signaling pathway.<\/jats:p>","DOI":"10.3390\/nu18142360","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T02:32:08Z","timestamp":1784514728000},"page":"2360","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Astilbin Alleviates Gouty Arthritis via Regulating NLRP3 Inflammasome and NF-\u03baB Signaling Pathway: A Comprehensive Study on In Vitro and In Vivo Experimental Models"],"prefix":"10.3390","volume":"18","author":[{"given":"Xiaoxi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Food Science and Nutrition, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"},{"name":"Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoyang","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenfen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7902-5002","authenticated-orcid":false,"given":"Daozong","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lin, Y., Luo, T., Weng, A., Huang, X., Yao, Y., Fu, Z., Li, Y., Liu, A., Li, X., and Chen, D. 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