{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T21:14:00Z","timestamp":1792271640589,"version":"4.4.0"},"reference-count":31,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["RS2022RD010270"],"award-info":[{"award-number":["RS2022RD010270"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]},{"award":["RS2022RD010270"],"award-info":[{"award-number":["RS2022RD010270"]}],"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background\/Objectives: Atopic dermatitis (AD) is a chronic inflammatory skin disorder requiring sustainable therapeutic alternatives. Cannabis sativa L. is a valuable industrial crop rich in bioactive secondary metabolites; its potential as a standardized functional ingredient for promoting skin health has not yet been fully investigated. This study aimed to evaluate the therapeutic effects of a chemically characterized C. sativa inflorescence ethanol extract (CSE) on AD. Methods: To evaluate the efficacy of CSE, phytochemical profiling was performed using UPLC, and its underlying molecular mechanisms were investigated in a DNCB-induced mouse model. Results: UPLC analysis was employed to establish the phytochemical profile, identifying 15 cannabinoids and quantifying 8 major components. CBDA was the most abundant component, with a content of 261.79 mg\/g in the extract. In a DNCB-induced mouse model, CSE significantly reduced mast cell infiltration and serum IgE levels while downregulating Th2-associated cytokines. At the molecular level, CSE inhibited the activation of the MAPK, NLRP3 inflammasome, and JAK1\/STAT6 signaling pathways. Crucially, CSE treatment substantially increased the expression of skin barrier proteins, such as filaggrin and involucrin, thereby enhancing skin hydration. Conclusions: These findings suggest CSE as a high-value functional ingredient capable of ameliorating AD by modulating multi-target immune responses. This study provides a robust scientific basis for utilizing standardized C. sativa inflorescence as a potent functional ingredient or a nutraceutical agent for the management of chronic skin inflammatory conditions.<\/jats:p>","DOI":"10.3390\/nu18142382","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:17:52Z","timestamp":1784647072000},"page":"2382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cannabidiol-Dominant Cannabis sativa L. Inflorescence Extract Ameliorates Atopic Dermatitis by Modulating NLRP3 Inflammasome and JAK1\/STAT6 Signaling in DNCB-Induced Mice"],"prefix":"10.3390","volume":"18","author":[{"given":"Ji-Ye","family":"Han","sequence":"first","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan 54538, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do-Won","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan 54538, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osoung","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan 54538, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9347-8606","authenticated-orcid":false,"given":"Yun Jung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan 54538, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minji","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan 54538, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bori","family":"Lee","sequence":"additional","affiliation":[{"name":"The One Health Design, Iksan 54575, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3743-5030","authenticated-orcid":false,"given":"Soohyang","family":"Noh","sequence":"additional","affiliation":[{"name":"Graduate School of Techno Design, Kookmin University, Seoul 02707, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansoo","family":"Cho","sequence":"additional","affiliation":[{"name":"Graduate School of Techno Design, Kookmin University, Seoul 02707, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Mi","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan 54538, Republic of Korea"},{"name":"The One Health Design, Iksan 54575, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Salehi, A., Puchalski, K., Shokoohinia, Y., Zolfaghari, B., and Asgary, S. 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