{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T08:50:18Z","timestamp":1785919818657,"version":"3.56.0"},"reference-count":81,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"P&G Health Germany GmbH"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Peripheral neuropathy (PN) is one of the most common disorders of the peripheral nervous system, affecting around 10% of the general adult population. PN results in pain, paraesthesia, and sensory loss, negatively impacting patients\u2019 quality of life. The condition is characterised by damage to peripheral nerves and is often linked to risk factors, among which diabetes is the most common cause. The incidence rate of patients suffering from PN has significantly increased in the last three decades, yet there are currently no pharmacological treatments to reverse or prevent the development of PN. Therefore, neurotropic B vitamins, specifically vitamins B1, B6, and B12, which are essential for protecting nerves from damage, nerve regeneration, and maintaining nerve health, are promising treatment options. Other biofactors with therapeutic potential are vitamin D3, which has been described as a regulator of neuroplasticity and neuroinflammation with indications to promote neuronal regeneration following injury, as well as alpha-lipoic acid (ALA), a well-described treatment option in PN. Methods: The regenerative capacity of vitamins B1, B6, B12, and D3 and ALA was determined following neurite degeneration in vitro induced by either hydrogen peroxide (H2O2) or homocysteine. Results: In line with previous studies, the results show that a combination of vitamins B1, B6, and B12 has a significantly better effect on neurite extension following homocysteine-induced degeneration. In H2O2-insulted neural cells, the combination of all three neurotropic B vitamins was also shown to be superior to that of individual B vitamin treatment, demonstrated by longer neurite length after insult. Vitamin D3 also demonstrated a neuro-regenerative effect on neurites in healthy and insulted neural cells, whereas ALA only appears to provide a protective effect, preventing ongoing damage and supporting neurite outgrowth in healthy cells. No synergistic effects were seen with any treatment combinations. Conclusions: We have established an in vitro model of homocysteine-induced degeneration, and using this model alongside H2O2-induced degeneration, we provide evidence of the neurite regenerative capacity of vitamins B1, B6, B12, and D3.<\/jats:p>","DOI":"10.3390\/nu18152555","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T08:14:46Z","timestamp":1785917686000},"page":"2555","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Effects of Neurotropic B Vitamins, Vitamin D3, and Alpha-Lipoic Acid in In Vitro Models of Neurodegeneration"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8884-7411","authenticated-orcid":false,"given":"Christian","family":"Viel","sequence":"first","affiliation":[{"name":"German Innovation Center, P&G Health Germany GmbH, Sulzbacher Stra\u00dfe 40, 65824 Schwalbach am Taunus, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK"},{"name":"UCL Centre for Nerve Engineering, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0734-9240","authenticated-orcid":false,"given":"Ryan P.","family":"Trueman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9058-9303","authenticated-orcid":false,"given":"Arnaud J.","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0041-505X","authenticated-orcid":false,"given":"Melissa L. D.","family":"Rayner","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK"},{"name":"UCL Centre for Nerve Engineering, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1080\/03007995.2024.2352852","article-title":"Prevalence and Disease Burden of Peripheral Neuropathy in the General Population in Mexico City: A Cross-Sectional Epidemiological Study","volume":"40","author":"Mijangos","year":"2024","journal-title":"Curr. Med. Res. Opin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s42466-020-00064-2","article-title":"Diagnosis of Peripheral Neuropathy","volume":"2","author":"Lehmann","year":"2020","journal-title":"Neurol. Res. Pract."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/B978-0-12-802973-2.00015-X","article-title":"Peripheral neuropathies","volume":"138","author":"Hanewinckel","year":"2016","journal-title":"Handb. Clin. Neurol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1001\/jama.2015.13611","article-title":"Distal Symmetric Polyneuropathy: A Review","volume":"314","author":"Callaghan","year":"2015","journal-title":"JAMA"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.molmed.2016.03.008","article-title":"Sodium Channels, Mitochondria, and Axonal Degeneration in Peripheral Neuropathy","volume":"22","author":"Persson","year":"2016","journal-title":"Trends Mol. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1002\/dneu.22710","article-title":"Models and Treatments for Traumatic Optic Neuropathy and Demyelinating Optic Neuritis","volume":"79","author":"Bastakis","year":"2019","journal-title":"Dev. Neurobiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev.neuro.051508.135531","article-title":"Neuropathic Pain: A Maladaptive Response of the Nervous System to Damage","volume":"32","author":"Costigan","year":"2009","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.hcl.2013.04.002","article-title":"Nerve Physiology: Mechanisms of Injury and Recovery","volume":"29","author":"Menorca","year":"2013","journal-title":"Hand Clin."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1097\/AJP.0000000000001085","article-title":"Central Sensitization and Small-Fiber Neuropathy Are Associated in Patients with Fibromyalgia","volume":"39","author":"Dumolard","year":"2023","journal-title":"Clin. J. Pain"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Au, N.P.B., and Ma, C.H.E. (2022). Neuroinflammation, Microglia and Implications for Retinal Ganglion Cell Survival and Axon Regeneration in Traumatic Optic Neuropathy. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.860070"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s10286-019-00607-x","article-title":"Physical Activity and Dietary Interventions in Diabetic Neuropathy: A Systematic Review","volume":"29","author":"Zilliox","year":"2019","journal-title":"Clin. Auton. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/j.clinthera.2021.04.003","article-title":"Corneal Confocal Microscopy: A Biomarker for Diabetic Peripheral Neuropathy","volume":"43","author":"Petropoulos","year":"2021","journal-title":"Clin. Ther."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1.e1","DOI":"10.1016\/j.jaad.2013.06.055","article-title":"Diabetic Foot Ulcers: Part I. Pathophysiology and Prevention","volume":"70","author":"Alavi","year":"2014","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1111\/dme.13083","article-title":"Systematic Review of Treatments for Diabetic Peripheral Neuropathy","volume":"33","author":"Fakkel","year":"2016","journal-title":"Diabet. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.ejphar.2018.06.034","article-title":"Molecular Mechanism of Diabetic Neuropathy and Its Pharmacotherapeutic Targets","volume":"833","author":"Dewanjee","year":"2018","journal-title":"Eur. J. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e081217","DOI":"10.1136\/bmj-2024-081217","article-title":"Advances in the Pathophysiology and Treatment of Diabetic Peripheral Neuropathy","volume":"392","author":"Fridman","year":"2026","journal-title":"BMJ"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/bs.irn.2016.03.010","article-title":"Alternative Quantitative Tools in the Assessment of Diabetic Peripheral and Autonomic Neuropathy","volume":"127","author":"Vinik","year":"2016","journal-title":"Int. Rev. Neurobiol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Galiero, R., Caturano, A., Vetrano, E., Beccia, D., Brin, C., Alfano, M., Di Salvo, J., Epifani, R., Piacevole, A., and Tagliaferri, G. (2023). Peripheral Neuropathy in Diabetes Mellitus: Pathogenetic Mechanisms and Diagnostic Options. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24043554"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s40266-025-01267-5","article-title":"Diabetic Peripheral Neuropathy: New Diagnostics and Treatment Perspectives","volume":"43","author":"Jude","year":"2026","journal-title":"Drugs Aging"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1016\/S1474-4422(22)00188-0","article-title":"Towards Prevention of Diabetic Peripheral Neuropathy: Clinical Presentation, Pathogenesis, and New Treatments","volume":"21","author":"Elafros","year":"2022","journal-title":"Lancet Neurol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"165","DOI":"10.2337\/diabetes.52.1.165","article-title":"Oxidative Injury and Apoptosis of Dorsal Root Ganglion Neurons in Chronic Experimental Diabetic Neuropathy","volume":"52","author":"Schmeichel","year":"2003","journal-title":"Diabetes"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"881","DOI":"10.2337\/diabetes.48.4.881","article-title":"Aberrant Neurofilament Phosphorylation in Sensory Neurons of Rats with Diabetic Neuropathy","volume":"48","author":"Fernyhough","year":"1999","journal-title":"Diabetes"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3239","DOI":"10.2337\/diabetes.53.12.3239","article-title":"Effects of Polyol Pathway Hyperactivity on Protein Kinase C Activity, Nociceptive Peptide Expression, and Neuronal Structure in Dorsal Root Ganglia in Diabetic Mice","volume":"53","author":"Uehara","year":"2004","journal-title":"Diabetes"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"830","DOI":"10.2337\/diabetes.54.3.830","article-title":"Experimental Diabetic Neuropathy with Spontaneous Recovery: Is There Irreparable Damage?","volume":"54","author":"Kennedy","year":"2005","journal-title":"Diabetes"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0168-8227(95)01091-Q","article-title":"Natural Course of Diabetic Peripheral Neuropathy in Spontaneous-Onset Diabetic Chinese Hamsters","volume":"28","author":"Hirano","year":"1995","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.nbd.2006.01.017","article-title":"Mitochondria in DRG Neurons Undergo Hyperglycemic Mediated Injury through Bim, Bax and the Fission Protein Drp1","volume":"23","author":"Leinninger","year":"2006","journal-title":"Neurobiol. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0014-2999(99)00356-8","article-title":"Glucose-Induced Oxidative Stress and Programmed Cell Death in Diabetic Neuropathy","volume":"375","author":"Greene","year":"1999","journal-title":"Eur. J. Pharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"143","DOI":"10.4103\/0973-3930.57344","article-title":"A Brief Review of in Vitro Models of Diabetic Neuropathy","volume":"29","author":"Hattangady","year":"2009","journal-title":"Int. J. Diabetes Dev. Ctries."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Slanzi, A., Iannoto, G., Rossi, B., Zenaro, E., and Constantin, G. (2020). In Vitro Models of Neurodegenerative Diseases. Front. Cell Dev. Biol., 8.","DOI":"10.3389\/fcell.2020.00328"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Segeritz, C.P., and Vallier, L. (2017). Cell Culture: Growing Cells as Model Systems In Vitro. Basic Science Methods for Clinical Researchers, Academic Press.","DOI":"10.1016\/B978-0-12-803077-6.00009-6"},{"key":"ref_31","first-page":"1000667","article-title":"Exploring the Effect of Vitamins B1, B6 and B12 on Neurite Regeneration Using a 3D Co-Culture Model of Neurodegeneration","volume":"11","author":"Smith","year":"2023","journal-title":"Int. J. Phys. Med. Rehabil."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Chabas, J.-F., Stephan, D., Marqueste, T., Garcia, S., Lavaut, M.-N., Nguyen, C., Legre, R., Khrestchatisky, M., Decherchi, P., and Feron, F. (2013). Cholecalciferol (Vitamin D3) Improves Myelination and Recovery after Nerve Injury. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0065034"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S1043-2760(01)00547-1","article-title":"New Clues about Vitamin D Functions in the Nervous System","volume":"13","author":"Garcion","year":"2002","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Lu, X., Chen, Z., Lu, J., and Watsky, M.A. (2023). Effects of 1,25-Vitamin D3 and 24,25-Vitamin D3 on Corneal Nerve Regeneration in Diabetic Mice. Biomolecules, 13.","DOI":"10.3390\/biom13121754"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"7211","DOI":"10.1007\/s12035-024-03989-w","article-title":"Tremendous Fidelity of Vitamin D3 in Age-Related Neurological Disorders","volume":"61","author":"Skv","year":"2024","journal-title":"Mol. Neurobiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"323","DOI":"10.2174\/0113816128281314231219113942","article-title":"Vitamin D as a Modulator of Neuroinflammation: Implications for Brain Health","volume":"30","author":"Manucha","year":"2024","journal-title":"Curr. Pharm. Des."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"El Soury, M., Fornasari, B.E., Carta, G., Zen, F., Haastert-Talini, K., and Ronchi, G. (2021). The Role of Dietary Nutrients in Peripheral Nerve Regeneration. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22147417"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1900\/RDS.2009.6.230","article-title":"Alpha-Lipoic Acid and Diabetic Neuropathy","volume":"6","author":"Vallianou","year":"2009","journal-title":"Rev. Diabet. Stud."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1053\/meta.2001.24914","article-title":"The Effects of Treatment with Alpha-Lipoic Acid or Evening Primrose Oil on Vascular Hemostatic and Lipid Risk Factors, Blood Flow, and Peripheral Nerve Conduction in the Streptozotocin-Diabetic Rat","volume":"50","author":"Ford","year":"2001","journal-title":"Metabolism"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1111\/j.1464-5491.2004.01109.x","article-title":"Treatment of Symptomatic Diabetic Polyneuropathy with the Antioxidant Alpha-Lipoic Acid: A Meta-Analysis","volume":"21","author":"Ziegler","year":"2004","journal-title":"Diabet. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2365","DOI":"10.2337\/dc06-1216","article-title":"Oral Treatment with Alpha-Lipoic Acid Improves Symptomatic Diabetic Polyneuropathy: The SYDNEY 2 Trial","volume":"29","author":"Ziegler","year":"2006","journal-title":"Diabetes Care"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2994","DOI":"10.1016\/j.febslet.2006.04.088","article-title":"Mechanisms of Homocysteine Neurotoxicity in Neurodegenerative Diseases with Special Reference to Dementia","volume":"580","author":"Obeid","year":"2006","journal-title":"FEBS Lett."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"58","DOI":"10.17756\/jnen.2020-076","article-title":"Homocysteine and Peripheral Neuropathy","volume":"6","author":"Hsu","year":"2020","journal-title":"J. Neurol. Exp. Neurosci."},{"key":"ref_44","first-page":"e101955","article-title":"Relationship Between Elevated Plasma Homocysteine Levels and Severity of Peripheral Neuropathy in Patients with Type 2 Diabetes Mellitus: A Cross-Sectional Analysis","volume":"18","author":"Toqeer","year":"2026","journal-title":"Cureus"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"133","DOI":"10.2217\/nmt-2015-0008","article-title":"The Relationship between Hyperhomocysteinemia and Neurodegeneration","volume":"6","author":"Bonetti","year":"2016","journal-title":"Neurodegener. Dis. Manag."},{"key":"ref_46","first-page":"1000001","article-title":"Transcriptome Changes and Neuronal Degeneration in an In Vitro Model of B Vitamin Depletion","volume":"10","author":"Banek","year":"2022","journal-title":"Int. J. Phys. Med. Rehabil."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Cuyubamba, O., Braga, C.P., Swift, D., Stickney, J.T., and Viel, C. (2025). The Combination of Neurotropic Vitamins B1, B6, and B12 Enhances Neural Cell Maturation and Connectivity Superior to Single B Vitamins. Cells, 14.","DOI":"10.3390\/cells14070477"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1007\/s11626-025-01024-3","article-title":"The Combination of Neurotropic B Vitamins (B1, B6, and B12) Is Superior to Individual B Vitamins in Promoting Neurite Growth in Vitro","volume":"61","author":"Rayner","year":"2025","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Faye, P.A., Poumeaud, F., Miressi, F., Lia, A.S., Demiot, C., Magy, L., Favreau, F., and Sturtz, F.G. (2019). Focus on 1,25-Dihydroxyvitamin D3 in the Peripheral Nervous System. Front. Neurosci., 13.","DOI":"10.3389\/fnins.2019.00348"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1038\/s41419-020-03355-3","article-title":"The Redox Language in Neurodegenerative Diseases: Oxidative Post-Translational Modifications by Hydrogen Peroxide","volume":"12","author":"Lee","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1089\/ars.2021.0152","article-title":"Oxidative Stress and Mitochondrial Dysfunction Associated with Peripheral Neuropathy in Type 1 Diabetes","volume":"37","author":"Eftekharpour","year":"2022","journal-title":"Antioxid. Redox Signal."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1016\/j.mayocp.2024.02.024","article-title":"Diagnosing Peripheral Neuropathy in Patients with Alcohol Use Disorder","volume":"99","author":"Paul","year":"2024","journal-title":"Mayo Clin. Proc."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Starobova, H., and Vetter, I. (2017). Pathophysiology of Chemotherapy-Induced Peripheral Neuropathy. Front. Mol. Neurosci., 10.","DOI":"10.3389\/fnmol.2017.00174"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1001\/jamaneurol.2016.3745","article-title":"Association Between Metabolic Syndrome Components and Polyneuropathy in an Obese Population","volume":"73","author":"Callaghan","year":"2016","journal-title":"JAMA Neurol."},{"key":"ref_56","first-page":"165","article-title":"Effect of Lifestyle Interventions on Diabetic Peripheral Neuropathy in Patients with Type 2 Diabetes, Result of a Randomized Clinical Trial","volume":"30","author":"Ghavami","year":"2018","journal-title":"J. Turk. Soc. Algol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/S2213-8587(19)30081-6","article-title":"Diabetic Peripheral Neuropathy: Advances in Diagnosis and Strategies for Screening and Early Intervention","volume":"7","author":"Selvarajah","year":"2019","journal-title":"Lancet Diabetes Endocrinol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"156521","DOI":"10.1155\/2012\/156521","article-title":"Mitigation of H(2)O(2)-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua Kunstleri (King) Kosterm","volume":"2012","author":"Chan","year":"2012","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.tiv.2017.07.009","article-title":"The Vitamin B6 Paradox: Supplementation with High Concentrations of Pyridoxine Leads to Decreased Vitamin B6 Function","volume":"44","author":"Vrolijk","year":"2017","journal-title":"Toxicol. In Vitro"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"111738","DOI":"10.1016\/j.nut.2022.111738","article-title":"Problematic Rise of Vitamin B6 Supplementation Overuse and Potential Risk to Bariatric Surgery Patients","volume":"102","author":"Bossard","year":"2022","journal-title":"Nutrition"},{"key":"ref_61","first-page":"100004","article-title":"Neurogenic Voiding Dysfunction Induced by Vitamin B6 Overdose","volume":"1","author":"Crul","year":"2022","journal-title":"Cont. Rep."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"7888243","DOI":"10.1155\/bn\/7888243","article-title":"Toxic Effects of Excess Vitamins A, B6, and Folic Acid on the Nervous System","volume":"2025","author":"Sun","year":"2025","journal-title":"Behav. Neurol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"97","DOI":"10.2147\/DHPS.S499941","article-title":"Expert Consensus on Vitamin B6 Therapeutic Use for Patients: Guidance on Safe Dosage, Duration and Clinical Management","volume":"17","author":"Schellack","year":"2025","journal-title":"Drug Healthc. Patient Saf."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"20587392211014052","DOI":"10.1177\/20587392211014053","article-title":"Interferon Alpha Serum Level Association with Low Vitamin D Levels in Chinese Patients with Primary Sj\u00f6gren\u2019s Syndrome","volume":"19","author":"Zheng","year":"2021","journal-title":"Eur. J. Inflamm."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1111\/nan.12020","article-title":"Review: The Role of Vitamin D in Nervous System Health and Disease","volume":"39","author":"DeLuca","year":"2013","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Sun, X., Yang, X., Zhu, X., Ma, Y., Li, X., Zhang, Y., Liu, Q., Fan, C., Zhang, M., and Xu, B. (2024). Association of Vitamin D Deficiency and Subclinical Diabetic Peripheral Neuropathy in Type 2 Diabetes Patients. Front. Endocrinol., 15.","DOI":"10.3389\/fendo.2024.1354511"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/dme.13798","article-title":"Reduced Vitamin D Levels in Painful Diabetic Peripheral Neuropathy","volume":"36","author":"Shillo","year":"2019","journal-title":"Diabet. Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"7928083","DOI":"10.1155\/2017\/7928083","article-title":"Improvement in Neuropathy Specific Quality of Life in Patients with Diabetes after Vitamin D Supplementation","volume":"2017","author":"Alam","year":"2017","journal-title":"J. Diabetes Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.dsx.2018.12.014","article-title":"Dose Vitamin D Supplementations Improve Peripheral Diabetic Neuropathy? A before-after Clinical Trial","volume":"13","author":"Mozafari","year":"2019","journal-title":"Diabetes Metab. Syndr. Clin. Res. Rev."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.dsx.2016.08.026","article-title":"Homocysteine Is a Marker for Metabolic Syndrome and Atherosclerosis","volume":"11","author":"Sreckovic","year":"2017","journal-title":"Diabetes Metab. Syndr. Clin. Res. Rev."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/S0895-7061(01)02165-3","article-title":"Prevalence of Hyperhomocysteinemia in an Elderly Population","volume":"15","author":"Janson","year":"2002","journal-title":"Am. J. Hypertens."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"5923","DOI":"10.1073\/pnas.94.11.5923","article-title":"Neurotoxicity Associated with Dual Actions of Homocysteine at the N-Methyl-D-Aspartate Receptor","volume":"94","author":"Lipton","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1007\/s11064-014-1338-7","article-title":"Toxic Effects of Mildly Elevated Homocysteine Concentrations in Neuronal-like Cells","volume":"39","author":"Gugliandolo","year":"2014","journal-title":"Neurochem. Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"S137","DOI":"10.1046\/j.1523-1755.63.s84.33.x","article-title":"Possible Mechanisms of Homocysteine Toxicity","volume":"63","author":"Perna","year":"2003","journal-title":"Kidney Int."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1476-511X-5-1","article-title":"Mining Literature for a Comprehensive Pathway Analysis: A Case Study for Retrieval of Homocysteine Related Genes for Genetic and Epigenetic Studies","volume":"5","author":"Sharma","year":"2006","journal-title":"Lipids Health Dis."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1139\/cjpp-2014-0353","article-title":"Alpha-Lipoic Acid: Molecular Mechanisms and Therapeutic Potential in Diabetes","volume":"93","author":"Rochette","year":"2015","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/cns.13207","article-title":"B Vitamins in the Nervous System: Current Knowledge of the Biochemical Modes of Action and Synergies of Thiamine, Pyridoxine, and Cobalamin","volume":"26","year":"2020","journal-title":"CNS Neurosci. Ther."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"2970","DOI":"10.1016\/j.clnu.2020.01.022","article-title":"A Systematic Review on the Efficacy of Vitamin D Supplementation on Diabetic Peripheral Neuropathy","volume":"39","author":"Yammine","year":"2020","journal-title":"Clin. Nutr."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2721","DOI":"10.1517\/14656566.2014.972935","article-title":"Efficacy of \u03b1-Lipoic Acid in Diabetic Neuropathy","volume":"15","author":"Papanas","year":"2014","journal-title":"Expert Opin. Pharmacother."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Tian, J., An, J., Song, L., Zhang, W., Chen, D., Zhang, T., and Ben, Y. (2026). Effect of ALA on Preventing Diabetic Peripheral Neuropathy in Rats through Mitochondrial Axonal Transport. PLoS ONE, 21.","DOI":"10.1371\/journal.pone.0346297"},{"key":"ref_81","first-page":"34","article-title":"Interaction between Thiamine Hydrochloride and Lipoic Acid","volume":"32","author":"Ammar","year":"1977","journal-title":"Pharmazie"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/15\/2555\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T08:24:44Z","timestamp":1785918284000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/18\/15\/2555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,5]]},"references-count":81,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["nu18152555"],"URL":"https:\/\/doi.org\/10.3390\/nu18152555","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,5]]}}}