{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:03:22Z","timestamp":1789621402396,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Beijing Natural Science Foundation","award":["7262170"],"award-info":[{"award-number":["7262170"]}]},{"name":"Beijing Natural Science Foundation","award":["7222249"],"award-info":[{"award-number":["7222249"]}]},{"name":"Science and technology project of Tibet Autonomous Region","award":["XZ202401ZY0092"],"award-info":[{"award-number":["XZ202401ZY0092"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Skin photoaging is primarily caused by prolonged ultraviolet (UV) exposure, which manifests as dryness and wrinkle formation. Recent studies highlight that beneficial microorganisms have garnered considerable attention for their emerging role in modulating host health, especially in skin photoaging. Methods: To investigate the role of two strains, Bifidobacterium animalis subsp. lactis BL-G101 (BL-G101) and Lactobacillus acidophilus LA-G80 (LA-G80), in UVB-induced photoaging, we conducted experiments on human Hs68 dermal fibroblasts. Using untargeted metabolomics, we explored the potential mechanisms underlying BL-G101 treatment in Hs68 cells by profiling differential metabolites in the intracellular and extracellular metabolomes. Results: Our results demonstrated that BL-G101 significantly inhibited UVB-induced reduction in collagen and hyaluronic acid (HA), whereas LA-G80 showed no notable effects. BL-G101 treatment also protects against UVB-induced oxidative stress, which was evidenced by significantly decreased MDA levels and enhanced SOD activity. Moreover, BL-G101 suppressed UVB-induced inflammation by significantly decreasing IL-1\u03b2 and TNF-\u03b1 levels. SA-\u03b2-gal staining revealed a reduction in senescent fibroblasts following BL-G101 treatment. Untargeted metabolomics suggested that the anti-photoaging effects of BL-G101 may involve the modulation of metabolic pathways of biosynthesis of cofactors, glycine, serine and threonine metabolism, and arginine biosynthesis. Conclusions: These preclinical findings demonstrate that BL-G101 reduces dermal photoaging, supporting its potential as a functional food ingredient against skin aging.<\/jats:p>","DOI":"10.3390\/nu18183013","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:31:57Z","timestamp":1789464717000},"page":"3013","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bifidobacterium animalis subsp. lactis BL-G101 Protects Against UVB-Induced Photoaging in HS68 Dermal Fibroblasts"],"prefix":"10.3390","volume":"18","author":[{"given":"Jingya","family":"Guo","sequence":"first","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2094-8822","authenticated-orcid":false,"given":"Ruixuan","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8498-120X","authenticated-orcid":false,"given":"Seong-Gook","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Food Engineering and Solar Salt Research Center, Mokpo National University, Muangun 58554, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunlun","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"},{"name":"Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture, Beijing 100083, China"},{"name":"Beijing Laboratory for Food Quality and Safety, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9309-2893","authenticated-orcid":false,"given":"Tao","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"},{"name":"Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture, Beijing 100083, China"},{"name":"Beijing Laboratory for Food Quality and Safety, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1038\/s43587-025-00901-6","article-title":"Skin health and biological aging","volume":"5","author":"Furman","year":"2025","journal-title":"Nat. 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