{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:58:32Z","timestamp":1791284312204,"version":"4.1.0"},"reference-count":36,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008871","name":"Yunnan International Joint R&D Center Project for Intelligent Diagnosis and Prevention and Control of Transboundary Animal Diseases","doi-asserted-by":"publisher","award":["202503AP140043"],"award-info":[{"award-number":["202503AP140043"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial International Science and Technology Commissioners","doi-asserted-by":"publisher","award":["202403AK140037"],"award-info":[{"award-number":["202403AK140037"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008871","name":"Yunnan Province Foreign Talent Recruitment Program","doi-asserted-by":"publisher","award":["202505AP120023"],"award-info":[{"award-number":["202505AP120023"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yunnan Revitalization Talent Support Program in Yunnan Province"},{"DOI":"10.13039\/501100008871","name":"Rural Vitalization Science and technology Project-Gejiu Cattle Industry Science and Technology Mission in Yunnan Province","doi-asserted-by":"publisher","award":["202304BI090007"],"award-info":[{"award-number":["202304BI090007"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: \u03b1-Amanitin (\u03b1-AMA) is the primary lethal toxin found in poisonous mushrooms of the genus Amanita; accidental ingestion can lead to severe liver injury, and there is currently no specific antidote. Due to its significant anti-inflammatory and antioxidant activity, Sulforaphane (SFN) has attracted increasing attention for its role in preventing liver injury. However, it remains unclear whether SFN can alleviate \u03b1-AMA-induced liver injury. This study aimed to elucidate the protective effects of SFN against \u03b1-AMA-induced liver injury and its potential molecular mechanisms. Methods and Results: The results showed that SFN significantly reduced serum ALT and AST levels in \u03b1-AMA-intoxicated mice (p &lt; 0.01), alleviated pathological damage such as hepatic edema, lobular rupture, and inflammatory infiltration, reduced apoptosis, lowered MDA levels, and simultaneously increased CAT activity (p &lt; 0.01). In AML-12 cells, SFN effectively reversed \u03b1-AMA-induced cellular damage (including reduced cell viability and decreased mitochondrial membrane potential). This study further found that SFN significantly upregulated the expression of Pink1 and Parkin. Furthermore, the overexpression of Pink1 significantly alleviated \u03b1-AMA-induced oxidative stress, mitochondrial damage, and apoptosis. Conclusions: The above results indicate that SFN may alleviate \u03b1-AMA-induced liver damage by promoting the expression of Pink1 and Parkin, improving mitochondrial dysfunction, and inhibiting apoptosis.<\/jats:p>","DOI":"10.3390\/nu18193282","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:46:29Z","timestamp":1791283589000},"page":"3282","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sulforaphane Alleviates \u03b1-Amanitin-Induced Liver Injury by Modulating Pink1-Parkin Pathway"],"prefix":"10.3390","volume":"18","author":[{"given":"Jian","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No. 95, Panlong District, Kunming 650051, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Yangyang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No. 95, Panlong District, Kunming 650051, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Zehui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No. 95, Panlong District, Kunming 650051, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Zhijie","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No. 95, Panlong District, Kunming 650051, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Zhengwang","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No. 95, Panlong District, Kunming 650051, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Peng","family":"Wu","sequence":"additional","affiliation":[{"name":"Yunnan Plateau Characteristic Agricultural Industry Research Institute, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Juyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No. 95, Panlong District, Kunming 650051, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Weijie","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No. 95, Panlong District, Kunming 650051, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Bundit","family":"Tengjaroenkul","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Yi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No. 95, Panlong District, Kunming 650051, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Xiaolong","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No. 95, Panlong District, Kunming 650051, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mao, B., Ren, B., Wu, J., Tang, X., Zhang, Q., Zhao, J., Zhang, L., Chen, W., and Cui, S. 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