{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:32:59Z","timestamp":1760243579368,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2013,9,2]],"date-time":"2013-09-02T00:00:00Z","timestamp":1378080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Associations between Giardia lamblia infection and low serum concentrations of zinc have been reported in young children. Interestingly, relatively few studies have examined the effects of different dietary zinc levels on the parasite-infected host. The aims of this study were to compare the growth performance and zinc status in response to varying levels of dietary zinc and to measure the antibody-mediated response of mice during G. lamblia infection. Male CD-1 mice were fed using 1 of 4 experimental diets: adequate-zinc (ZnA), low-zinc (ZnL), high-zinc (ZnH) and supplemented-zinc (ZnS) diet containing 30, 10, 223 and 1383 mg Zn\/kg respectively. After a 10 days feeding period, mice were inoculated orally with 5 \u00d7 106 G. lamblia trophozoites and were maintained on the assigned diet during the course of infection (30 days). Giardia-free mice fed ZnL diets were able to attain normal growth and antibody-mediated response. Giardia-infected mice fed ZnL and ZnA diets presented a significant growth retardation compared to non-infected controls. Zinc supplementation avoided this weight loss during G. lamblia infection and up-regulated the host\u2019s humoral immune response by improving the production of specific antibodies. Clinical outcomes of zinc supplementation during giardiasis included significant weight gain, higher anti-G. lamblia IgG antibodies and improved serum zinc levels despite the ongoing infection. A maximum growth rate and antibody-mediated response were attained in mice fed ZnH diet. No further increases in body weight, zinc status and humoral immune capacity were noted by feeding higher zinc levels (ZnS) than the ZnH diet. These findings probably reflect biological effect of zinc that could be of public health importance in endemic areas of infection.<\/jats:p>","DOI":"10.3390\/nu5093447","type":"journal-article","created":{"date-parts":[[2013,9,2]],"date-time":"2013-09-02T11:38:05Z","timestamp":1378121885000},"page":"3447-3460","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Dietary Zinc Manipulation on Growth Performance, Zinc Status and Immune Response during Giardia lamblia Infection: A Study in CD-1 Mice"],"prefix":"10.3390","volume":"5","author":[{"given":"Gemma","family":"I\u00f1igo-Figueroa","sequence":"first","affiliation":[{"name":"Departamento de Nutrici\u00f3n y Metabolismo, Coordinaci\u00f3n de Nutrici\u00f3n, Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo, A.C. Carretera a La Victoria Km 0.6, Hermosillo, Sonora C.P. 83304, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"M\u00e9ndez-Estrada","sequence":"additional","affiliation":[{"name":"Departamento de Nutrici\u00f3n y Metabolismo, Coordinaci\u00f3n de Nutrici\u00f3n, Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo, A.C. Carretera a La Victoria Km 0.6, Hermosillo, Sonora C.P. 83304, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Quihui-Cota","sequence":"additional","affiliation":[{"name":"Departamento de Nutrici\u00f3n P\u00fablica y Salud, Coordinaci\u00f3n de Nutrici\u00f3n, Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo, A.C. Carretera a La Victoria Km 0.6, Hermosillo,  Sonora C.P. 83304, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Vel\u00e1squez-Contreras","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad de Sonora. Blvd. Luis Encinas y Rosales s\/n, Hermosillo, Sonora C.P. 83000, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Garibay-Escobar","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad de Sonora. Blvd. Luis Encinas y Rosales s\/n, Hermosillo, Sonora C.P. 83000, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Canett-Romero","sequence":"additional","affiliation":[{"name":"Departamento de Investigaci\u00f3n y Posgrado en Alimentos, Universidad de Sonora. Blvd. Luis Encinas y Rosales s\/n, Hermosillo, Sonora C.P. 83000, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2452-0057","authenticated-orcid":false,"given":"Humberto","family":"Astiazar\u00e1n-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Nutrici\u00f3n y Metabolismo, Coordinaci\u00f3n de Nutrici\u00f3n, Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo, A.C. Carretera a La Victoria Km 0.6, Hermosillo, Sonora C.P. 83304, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/sj.ejcn.1601492","article-title":"Nutrition and the immune system from birth to old age","volume":"56","author":"Chandra","year":"2002","journal-title":"Eur. J. Clin. 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