{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:19:49Z","timestamp":1791112789121,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2014,2,21]],"date-time":"2014-02-21T00:00:00Z","timestamp":1392940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>New and sustainable sources of long-chain (LC, \u2265C20) omega-3 oils containing DHA (docosahexaenoic acid, 22:6\u03c93) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG) as the major lipid class in hexane extracts (96% of total lipid). Subsequent chloroform-methanol (CM) extraction recovered further lipid (~50% polar lipid, comprising glycolipids and phospholipids) and residual TAG. The main phospholipid species were phosphatidyl choline and phosphatidyl ethanolamine. The % DHA was: 6.8% (of total fatty acids) in the TAG-rich hexane extract and 4.2% in the polar lipid-rich CM extract. The relative level of ALA (\u03b1-linolenic acid, 18:3\u03c93) in  DHA-camelina seed was higher than the control. Major sterols in both DHA- and control camelina seeds were: sitosterol, campesterol, cholesterol, brassicasterol and isofucosterol. C16\u2013C22 fatty alcohols, including iso-branched and odd-chain alcohols were present, including high levels of iso-17:0, 17:0 and 19:0. Other alcohols present were: 16:0,  iso-18:0, 18:0 and 18:1 and the proportions varied between the hexane and CM extracts. These iso-branched odd-chain fatty alcohols, to our knowledge, have not been previously reported. These components may be derived from wax esters, or free fatty alcohols.<\/jats:p>","DOI":"10.3390\/nu6020776","type":"journal-article","created":{"date-parts":[[2014,2,21]],"date-time":"2014-02-21T11:55:11Z","timestamp":1392983711000},"page":"776-789","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Characterization of Oilseed Lipids from \u201cDHA-Producing Camelina sativa\u201d: A New Transformed Land Plant  Containing Long-Chain Omega-3 Oils"],"prefix":"10.3390","volume":"6","author":[{"given":"Maged","family":"Mansour","sequence":"first","affiliation":[{"name":"CSIRO Food Futures Flagship, Marine and Atmospheric Research, Castray Esplanade Hobart, Tasmania 7000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pushkar","family":"Shrestha","sequence":"additional","affiliation":[{"name":"CSIRO Food Futures Flagship, Plant Industry, P.O. Box 1600, Canberra ACT 2601, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srinivas","family":"Belide","sequence":"additional","affiliation":[{"name":"CSIRO Food Futures Flagship, Plant Industry, P.O. Box 1600, Canberra ACT 2601, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Petrie","sequence":"additional","affiliation":[{"name":"CSIRO Food Futures Flagship, Plant Industry, P.O. Box 1600, Canberra ACT 2601, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Nichols","sequence":"additional","affiliation":[{"name":"CSIRO Food Futures Flagship, Marine and Atmospheric Research, Castray Esplanade Hobart, Tasmania 7000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surinder","family":"Singh","sequence":"additional","affiliation":[{"name":"CSIRO Food Futures Flagship, Plant Industry, P.O. Box 1600, Canberra ACT 2601, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1159\/000228999","article-title":"Effects of fat and fatty acid intake on inflammatory and immune responses: A critical review","volume":"55","author":"Galli","year":"2009","journal-title":"Ann. Nutr. Metab."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1111\/nure.12066","article-title":"Omega-3 fatty acids influence mood in healthy and depressed individuals","volume":"71","author":"Giles","year":"2013","journal-title":"Nutr. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/nbu.12021","article-title":"Fish in the diet: A review","volume":"38","author":"Weichselbaum","year":"2013","journal-title":"Nutr. 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