{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T23:36:17Z","timestamp":1776728177487,"version":"3.51.2"},"reference-count":37,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2015,4,2]],"date-time":"2015-04-02T00:00:00Z","timestamp":1427932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background and Aims: Studies in South Asian population show that low maternal vitamin B12 associates with insulin resistance and small for gestational age in the offspring. Low vitamin B12 status is attributed to vegetarianism in these populations. It is not known whether low B12 status is associated with metabolic risk of the offspring in whites, where the childhood metabolic disorders are increasing rapidly. Here, we studied whether maternal B12 levels associate with metabolic risk of the offspring at birth. Methods: This is a  cross-sectional study of 91 mother-infant pairs (n = 182), of white Caucasian origin living in the UK. Blood samples were collected from white pregnant women at delivery and their newborns (cord blood). Serum vitamin B12, folate, homocysteine as well as the relevant metabolic risk factors were measured. Results: The prevalence of low serum vitamin B12 (&lt;191 ng\/L) and folate (&lt;4.6 \u03bcg\/L) were 40% and 11%, respectively. Maternal B12 was inversely associated with offspring\u2019s Homeostasis Model Assessment 2-Insulin Resistance (HOMA-IR), triglycerides, homocysteine and positively with HDL-cholesterol after adjusting for age and BMI. In regression analysis, after adjusting for likely confounders, maternal B12 is independently associated with neonatal HDL-cholesterol and homocysteine but not triglycerides or HOMA-IR. Conclusions: Our study shows that low B12 status  is common in white women and is independently associated with adverse cord  blood cholesterol.<\/jats:p>","DOI":"10.3390\/nu7042401","type":"journal-article","created":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T03:47:46Z","timestamp":1428378466000},"page":"2401-2414","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Low Maternal Vitamin B12 Status Is Associated with Lower Cord Blood HDL Cholesterol in White Caucasians Living  in the UK"],"prefix":"10.3390","volume":"7","author":[{"given":"Antonysunil","family":"Adaikalakoteswari","sequence":"first","affiliation":[{"name":"Warwick Medical School, University of Warwick, Coventry CV2 2DX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manu","family":"Vatish","sequence":"additional","affiliation":[{"name":"Nuffield Department of Obstetrics & Gynaecology, University of Oxford, Oxford OX3 9DU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Lawson","sequence":"additional","affiliation":[{"name":"Department of Pathology, Heartlands Hospital, Birmingham B9 5SS, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Wood","sequence":"additional","affiliation":[{"name":"Academic department of Diabetes and Metabolism, George Eliot Hospital, Nuneaton CV10 7DJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kavitha","family":"Sivakumar","sequence":"additional","affiliation":[{"name":"Warwick Medical School, University of Warwick, Coventry CV2 2DX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip","family":"McTernan","sequence":"additional","affiliation":[{"name":"Warwick Medical School, University of Warwick, Coventry CV2 2DX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig","family":"Webster","sequence":"additional","affiliation":[{"name":"Department of Pathology, Heartlands Hospital, Birmingham B9 5SS, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil","family":"Anderson","sequence":"additional","affiliation":[{"name":"Academic department of Diabetes and Metabolism, George Eliot Hospital, Nuneaton CV10 7DJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chittaranjan","family":"Yajnik","sequence":"additional","affiliation":[{"name":"Diabetes Research Centre, KEM Hospital, Pune 411011, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyanendra","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Warwick Medical School, University of Warwick, Coventry CV2 2DX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9566-2626","authenticated-orcid":false,"given":"Ponnusamy","family":"Saravanan","sequence":"additional","affiliation":[{"name":"Warwick Medical School, University of Warwick, Coventry CV2 2DX, UK"},{"name":"Academic department of Diabetes and Metabolism, George Eliot Hospital, Nuneaton CV10 7DJ, UK"},{"name":"WISDEM Centre, University Hospital Coventry and Warwickshire, Coventry CV2 2DX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1056\/NEJMoa1309753","article-title":"Incidence of childhood obesity in the united states","volume":"370","author":"Cunningham","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S310","DOI":"10.2337\/dc08-s273","article-title":"Metabolic complications of childhood obesity: Identifying and mitigating the risk","volume":"31","author":"Weiss","year":"2008","journal-title":"Diabetes Care"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1111\/aogs.12340","article-title":"Trends in the prevalence of gestational diabetes mellitus in southern sweden, 2003\u20132012","volume":"93","author":"Ignell","year":"2014","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lawlor, D.A., Benfield, L., Logue, J., Tilling, K., Howe, L.D., Fraser, A., Cherry, L., Watt, P., Ness, A.R., and Davey Smith, G. (2010). Association between general and central adiposity in childhood, and change in these, with cardiovascular risk factors in adolescence: Prospective cohort study. BMJ, 341.","DOI":"10.1136\/bmj.c6224"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1007\/s12291-012-0252-5","article-title":"Correlation of cord blood lipid heterogeneity in neonates with their anthropometry at birth","volume":"28","author":"Nayak","year":"2013","journal-title":"Indian J. Clin. Biochem. IJCB"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2886","DOI":"10.1001\/jama.2008.886","article-title":"Birth weight and risk of type 2 diabetes: A systematic review","volume":"300","author":"Whincup","year":"2008","journal-title":"JAMA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"S147","DOI":"10.2337\/dc07-s207","article-title":"Low birth weight as a risk factor for gestational diabetes, diabetes, and impaired glucose tolerance during pregnancy","volume":"30","author":"Pettitt","year":"2007","journal-title":"Diabetes Care"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1080\/03014460902980295","article-title":"Growth and chronic disease: Findings in the Helsinki birth cohort","volume":"36","author":"Barker","year":"2009","journal-title":"Ann. Hum. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.maturitas.2011.06.017","article-title":"Hungry in the womb: What are the consequences? Lessons from the dutch famine","volume":"70","author":"Roseboom","year":"2011","journal-title":"Maturitas"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s00125-007-0793-y","article-title":"Vitamin B12 and folate concentrations during pregnancy and insulin resistance in the offspring: The pune maternal nutrition study","volume":"51","author":"Yajnik","year":"2008","journal-title":"Diabetologia"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.3945\/jn.111.144717","article-title":"Low maternal vitamin B-12 status is associated with offspring insulin resistance regardless of antenatal micronutrient supplementation in rural Nepal","volume":"141","author":"Stewart","year":"2011","journal-title":"J. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1007\/s00125-013-3086-7","article-title":"Association between maternal folate concentrations during pregnancy and insulin resistance in Indian children","volume":"57","author":"Krishnaveni","year":"2014","journal-title":"Diabetologia"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.3945\/ajcn.112.056382","article-title":"High folate and low vitamin B-12 intakes during pregnancy are associated with small-for-gestational age infants in South Indian women: A prospective observational cohort study","volume":"98","author":"Dwarkanath","year":"2013","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1177\/1474651409358015","article-title":"Role of maternal vitamin B12 on the metabolic health of the offspring: A contributor to the diabetes epidemic?","volume":"10","author":"Saravanan","year":"2010","journal-title":"Br. J. Diabetes Vasc. Disease"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/ajcn\/80.5.1312","article-title":"Low ratio of S-adenosylmethionine to S-adenosylhomocysteine is associated with vitamin deficiency in Brazilian pregnant women and newborns","volume":"80","author":"Morita","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/ejcn.2010.294","article-title":"Relationship of folate, vitamin B12 and methylation of insulin-like growth factor-II in maternal and cord blood","volume":"65","author":"Ba","year":"2011","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"19351","DOI":"10.1073\/pnas.0707258104","article-title":"DNA methylation, insulin resistance, and blood pressure in offspring determined by maternal periconceptional B vitamin and methionine status","volume":"104","author":"Sinclair","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1002\/path.2881","article-title":"Methyl donor deficiency induces cardiomyopathy through altered methylation\/acetylation of PGC-1alpha by PRMT1 and SIRT1","volume":"225","author":"Garcia","year":"2011","journal-title":"J. Pathol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.jnutbio.2012.01.004","article-title":"Maternal dietary folate and\/or vitamin B12 restrictions alter body composition (adiposity) and lipid metabolism in Wistar rat offspring","volume":"24","author":"Kumar","year":"2013","journal-title":"J. Nutr. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Adaikalakoteswari, A., Jayashri, R., Sukumar, N., Venkataraman, H., Pradeepa, R., Gokulakrishnan, K., Anjana, R., McTernan, P.G., Tripathi, G., and Patel, V. (2014). Vitamin B12 deficiency is associated with adverse lipid profile in Europeans and Indians with type 2 diabetes. Cardiovasc. Diabetol., 13.","DOI":"10.1186\/s12933-014-0129-4"},{"key":"ref_21","first-page":"37","article-title":"Population levels of B12 and folic acid: Do we need to consider B12 fortification to prevent gestational diabetes and cardiovascular risk?","volume":"27","author":"Saravanan","year":"2010","journal-title":"Diabet. Med."},{"key":"ref_22","first-page":"S151","article-title":"B12 deficiency is more common than folate deficiency in early pregnancy: Do we need to consider B12 fortification?","volume":"53","author":"Saravanan","year":"2010","journal-title":"Diabetologia"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1111\/j.0902-4441.2006.t01-1-EJH2550.x","article-title":"Cobalamin status during normal pregnancy and postpartum: A longitudinal study comprising 406 Danish women","volume":"76","author":"Milman","year":"2006","journal-title":"Eur. J. Haematol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1111\/j.1600-0609.2005.00606.x","article-title":"Erythrocyte folate, plasma folate and plasma homocysteine during normal pregnancy and postpartum: A longitudinal study comprising 404 Danish women","volume":"76","author":"Milman","year":"2006","journal-title":"Eur. J. Haematol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1093\/clinchem\/45.9.1517","article-title":"Method for the determination of total homocysteine in plasma and urine by stable isotope dilution and electrospray tandem mass spectrometry","volume":"45","author":"Magera","year":"1999","journal-title":"Clin. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1111\/dme.12390","article-title":"The role of the one-carbon cycle in the developmental origins of type 2 diabetes and obesity","volume":"31","author":"Finer","year":"2014","journal-title":"Diabet. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1093\/ajcn\/87.2.391","article-title":"Homocysteine concentration, related B vitamins, and betaine in pregnant women recruited to the Seychelles child development study","volume":"87","author":"Wallace","year":"2008","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_28","unstructured":"United States Department of Agriculture (2003). USDA Table of Nutrient Retention Factors, Release 5, Available online:http:\/\/www.ars.usda.gov\/Main\/docs.htm?docid=9448."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Adaikalakoteswari, A., Finer, S., Voyias, P.D., McCarthy, C.M., Vatish, M., Moore, J., Smart-Halajko, M., Bawazeer, N., Al-Daghri, N.M., and McTernan, P.G. (2015). Vitamin B12 insufficiency induces cholesterol biosynthesis by limiting S-adenosylmethionine and modulating the methylation of SREBF1 and LDLR genes. Clin. Epigenetics, 7.","DOI":"10.1186\/s13148-015-0046-8"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.2337\/dc08-0039","article-title":"Maternal lipids as strong determinants of fetal environment and growth in pregnancies with gestational diabetes mellitus","volume":"31","author":"Graf","year":"2008","journal-title":"Diabetes Care"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1111\/j.1464-5491.2011.03346.x","article-title":"Differences in the implications of maternal lipids on fetal metabolism and growth between gestational diabetes mellitus and control pregnancies","volume":"28","author":"Meitzner","year":"2011","journal-title":"Diabet. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1093\/ajcn\/82.4.836","article-title":"Choline and homocysteine interrelations in umbilical cord and maternal plasma at delivery","volume":"82","author":"Molloy","year":"2005","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1136\/bmj.325.7374.1202","article-title":"Homocysteine and cardiovascular disease: Evidence on causality from a meta-analysis","volume":"325","author":"Wald","year":"2002","journal-title":"BMJ"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1017\/S0029665111003338","article-title":"Maternal nutritional status, C(1) metabolism and offspring DNA methylation: A review of current evidence in human subjects","volume":"71","author":"Cox","year":"2012","journal-title":"Proc. Nutr. Soc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1042\/bj2320177","article-title":"Regulation of carnitine palmitoyltransferase activity by malonyl-CoA in mitochondria from sheep liver, a tissue with a low capacity for fatty acid synthesis","volume":"232","author":"Brindle","year":"1985","journal-title":"Biochem. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1079\/BJN2000209","article-title":"Longitudinal vitamin and homocysteine levels in normal pregnancy","volume":"85","author":"Cikot","year":"2001","journal-title":"Br. J. Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1056\/NEJMc1204070","article-title":"Failures of cobalamin assays in pernicious anemia","volume":"367","author":"Carmel","year":"2012","journal-title":"N. Engl. J. Med."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/7\/4\/2401\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:44:17Z","timestamp":1760215457000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/7\/4\/2401"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,2]]},"references-count":37,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2015,4]]}},"alternative-id":["nu7042401"],"URL":"https:\/\/doi.org\/10.3390\/nu7042401","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,2]]}}}