{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T17:55:41Z","timestamp":1793382941501,"version":"4.5.0"},"reference-count":26,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T00:00:00Z","timestamp":1462924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>State of the art. Osteoarthritis (OA) is a chronic articular disease characterized by cartilage degradation and osteophyte formation. OA physiopathology is multifactorial and involves mechanical and hereditary factors. So far, there is neither preventive medicine to delay cartilage breakdown nor curative treatment. Objectives. To investigate pro-inflammatory paracrine interactions between human primary chondrocytes and macrophages following interleukin-1-\u03b2 (IL-1\u03b2) treatment; to evaluate the molecular mechanism responsible for the inhibitory effect of resveratrol. Results. The activation of NF-\u03baB in chondrocytes by IL-1\u03b2 induced IL-6 secretion. The latter will then activate STAT3 protein in macrophages. Moreover, STAT3 was able to positively regulate IL-6 secretion, as confirmed by the doubling level of IL-6 in the coculture compared to macrophage monoculture. These experiments confirm the usefulness of the coculture model in the inflammatory arthritis-linked process as a closer biological situation to the synovial joint than separated chondrocytes and macrophages. Il also demonstrated the presence of an inflammatory amplification loop induced by IL-1\u03b2. Resveratrol showed a strong inhibitory effect on the pro-inflammatory marker secretion. The decrease of IL-6 secretion is dependent on the NF\u03baB inhibition in the chondrocytes. Such reduction of the IL-6 level can limit STAT3 activation in the macrophages, leading to the interruption of the inflammatory amplification loop. Conclusion. These results increase our understanding of the anti-inflammatory actions of resveratrol and open new potential approaches to prevent and treat osteoarthritis.<\/jats:p>","DOI":"10.3390\/nu8050280","type":"journal-article","created":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T10:53:59Z","timestamp":1462964039000},"page":"280","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Resveratrol Interferes with IL1-\u03b2-Induced Pro-Inflammatory Paracrine Interaction between Primary Chondrocytes and Macrophages"],"prefix":"10.3390","volume":"8","author":[{"given":"Emeric","family":"Limagne","sequence":"first","affiliation":[{"name":"Laboratoire de Lipides, Nutrition, Cancer, Universit\u00e9 de Bourgogne-Franche Comt\u00e9, Dijon F21000, France"},{"name":"Laboratoire de Biochimie du Peroxysome, Inflammation et M\u00e9tabolisme des Lipides (BioPeroxIL EA 7270), Facult\u00e9 des Sciences Gabriel, Dijon F21000, France"},{"name":"\u201cChemotherapy, Lipid Metabolism and Antitumoral Immune Response\u201d Team, Faculty of Health Sciences, INSERM (Institut National de la Sant\u00e9 et de la recherch\u00e9 M\u00e9dicale) Research Center U866, Dijon F21000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan","family":"Lan\u00e7on","sequence":"additional","affiliation":[{"name":"Laboratoire de Lipides, Nutrition, Cancer, Universit\u00e9 de Bourgogne-Franche Comt\u00e9, Dijon F21000, France"},{"name":"Laboratoire de Biochimie du Peroxysome, Inflammation et M\u00e9tabolisme des Lipides (BioPeroxIL EA 7270), Facult\u00e9 des Sciences Gabriel, Dijon F21000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Delmas","sequence":"additional","affiliation":[{"name":"Laboratoire de Lipides, Nutrition, Cancer, Universit\u00e9 de Bourgogne-Franche Comt\u00e9, Dijon F21000, France"},{"name":"\u201cChemotherapy, Lipid Metabolism and Antitumoral Immune Response\u201d Team, Faculty of Health Sciences, INSERM (Institut National de la Sant\u00e9 et de la recherch\u00e9 M\u00e9dicale) Research Center U866, Dijon F21000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustapha","family":"Cherkaoui-Malki","sequence":"additional","affiliation":[{"name":"Laboratoire de Lipides, Nutrition, Cancer, Universit\u00e9 de Bourgogne-Franche Comt\u00e9, Dijon F21000, France"},{"name":"Laboratoire de Biochimie du Peroxysome, Inflammation et M\u00e9tabolisme des Lipides (BioPeroxIL EA 7270), Facult\u00e9 des Sciences Gabriel, Dijon F21000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6794-2690","authenticated-orcid":false,"given":"Norbert","family":"Latruffe","sequence":"additional","affiliation":[{"name":"Laboratoire de Lipides, Nutrition, Cancer, Universit\u00e9 de Bourgogne-Franche Comt\u00e9, Dijon F21000, France"},{"name":"Laboratoire de Biochimie du Peroxysome, Inflammation et M\u00e9tabolisme des Lipides (BioPeroxIL EA 7270), Facult\u00e9 des Sciences Gabriel, Dijon F21000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,5,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1002\/art.38401","article-title":"Activation of matrix metalloproteinases 2, 9, and 13 by activated protein C in human osteoarthritic cartilage chondrocytes","volume":"66","author":"Jackson","year":"2014","journal-title":"Arthritis Rheum."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.joca.2012.11.017","article-title":"SIRT1, a class III histone deacetylase, regulates TNF-\u03b1-induced inflammation in human chondrocytes","volume":"21","author":"Moon","year":"2013","journal-title":"Osteoarthr. 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