{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T08:02:53Z","timestamp":1768982573652,"version":"3.49.0"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2016,7,2]],"date-time":"2016-07-02T00:00:00Z","timestamp":1467417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The recruitment and infiltration of classical monocytes into damaged muscle is critical for optimal tissue remodeling. This study examined the effects of an amino acid supplement on classical monocyte recruitment following an acute bout of lower body resistance exercise. Ten resistance-trained men (24.7 \u00b1 3.4 years; 90.1 \u00b1 11.3 kg; 176.0 \u00b1 4.9 cm) ingested supplement (SUPP) or placebo (PL) immediately post-exercise in a randomized, cross-over design. Blood samples were obtained at baseline (BL), immediately (IP), 30-min (30P), 1-h (1H), 2-h (2H), and 5-h (5H) post-exercise to assess plasma concentrations of monocyte chemoattractant protein 1 (MCP-1), myoglobin, cortisol and insulin concentrations; and expressions of C-C chemokine receptor-2 (CCR2), and macrophage-1 antigen (CD11b) on classical monocytes. Magnitude-based inferences were used to provide inferences on the true effects of SUPP compared to PL. Changes in myoglobin, cortisol, and insulin concentrations were similar between treatments. Compared to PL, plasma MCP-1 was \u201cvery likely greater\u201d (98.1% likelihood effect) in SUPP at 2H. CCR2 expression was \u201clikely greater\u201d at IP (84.9% likelihood effect), \u201clikely greater\u201d at 1H (87.7% likelihood effect), \u201cvery likely greater\u201d at 2H (97.0% likelihood effect), and \u201clikely greater\u201d at 5H (90.1% likelihood effect) in SUPP, compared to PL. Ingestion of SUPP did not influence CD11b expression. Ingestion of an amino acid supplement immediately post-exercise appears to help maintain plasma MCP-1 concentrations and augment CCR2 expression in resistance trained men.<\/jats:p>","DOI":"10.3390\/nu8070409","type":"journal-article","created":{"date-parts":[[2016,7,4]],"date-time":"2016-07-04T10:04:21Z","timestamp":1467626661000},"page":"409","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The Effect of Post-Resistance Exercise Amino Acids on Plasma MCP-1 and CCR2 Expression"],"prefix":"10.3390","volume":"8","author":[{"given":"Adam","family":"Wells","sequence":"first","affiliation":[{"name":"Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Jajtner","sequence":"additional","affiliation":[{"name":"Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa","family":"Varanoske","sequence":"additional","affiliation":[{"name":"Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Church","sequence":"additional","affiliation":[{"name":"Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Health Professions, Hofstra University, Hempstead, NY 11549, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Townsend","sequence":"additional","affiliation":[{"name":"Department of Kinesiology, Lipscomb University, Nashville, TN 37204, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carleigh","family":"Boone","sequence":"additional","affiliation":[{"name":"Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayla","family":"Baker","sequence":"additional","affiliation":[{"name":"Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 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Rev. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1097\/JES.0b013e318190ebdb","article-title":"Dual and beneficial roles of macrophages during skeletal muscle regeneration","volume":"37","author":"Chazaud","year":"2009","journal-title":"Exerc. Sport Sci. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4118","DOI":"10.1111\/febs.12166","article-title":"Monocyte\/macrophage interactions with myogenic precursor cells during skeletal muscle regeneration","volume":"280","author":"Saclier","year":"2013","journal-title":"FEBS J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/nri2779","article-title":"Phagocyte partnership during the onset and resolution of inflammation","volume":"10","author":"Soehnlein","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1084\/jem.20070075","article-title":"Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis","volume":"204","author":"Arnold","year":"2007","journal-title":"J. Exp. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1084\/jem.187.4.601","article-title":"Abnormalities in monocyte recruitment and cytokine expression in monocyte chemoattractant protein 1-deficient mice","volume":"187","author":"Lu","year":"1998","journal-title":"J. Exp. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1096\/fj.10-171579","article-title":"Macrophages recruited via CCR2 produce insulin-like growth factor-1 to repair acute skeletal muscle injury","volume":"25","author":"Lu","year":"2011","journal-title":"FASEB J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1034\/j.1600-0838.2003.02477.x","article-title":"Recent advances in the understanding of the repeated bout effect: The protective effect against muscle damage from a single bout of eccentric exercise","volume":"13","author":"McHugh","year":"2003","journal-title":"Scand. J. Med. Sci. Sports"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"424","DOI":"10.3389\/fphys.2015.00424","article-title":"Skeletal muscle inflammation following repeated bouts of lengthening contractions in humans","volume":"6","author":"Deyhle","year":"2016","journal-title":"Front. Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/nri3901","article-title":"Regulation of innate immune cell function by mTOR","volume":"15","author":"Weichhart","year":"2015","journal-title":"Nat. Rev. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1161\/CIRCRESAHA.114.302313","article-title":"Disruption of mammalian target of rapamycin complex 1 in macrophages decreases chemokine gene expression and atherosclerosis","volume":"114","author":"Ai","year":"2014","journal-title":"Circ. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1083\/jcb.201102090","article-title":"Review series: TOR kinase complexes and cell migration","volume":"194","author":"Liu","year":"2011","journal-title":"J. Cell Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1519\/JSC.0b013e3181c7c655","article-title":"Amino acid supplements and recovery from high-intensity resistance training","volume":"24","author":"Sharp","year":"2010","journal-title":"J. Strength. Cond. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1007\/s00726-015-1937-x","article-title":"Key mediators of intracellular amino acids signaling to mTORC1 activation","volume":"47","author":"Duan","year":"2015","journal-title":"Amino Acids"},{"key":"ref_16","unstructured":"Gravettier, F.J., and Wallnau, L.B. (1996). Statistics for Behavioral Sciences, West Publishing Co.. [4th ed.]."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1007\/s00421-013-2693-9","article-title":"Effect of cryotherapy on muscle recovery and inflammation following a bout of damaging exercise","volume":"113","author":"Crystal","year":"2013","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1080\/07303084.1993.10606684","article-title":"Strength testing\u2014Predicting a one-rep max from reps-to-fatigue","volume":"64","author":"Brzycki","year":"1993","journal-title":"J. Phys. Educ. Recreat. Dance"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1152\/jappl.1974.37.2.247","article-title":"Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration","volume":"37","author":"Dill","year":"1974","journal-title":"J. Appl. Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3389\/fphys.2015.00048","article-title":"TNF-Alpha and TNFR1 responses to recovery therapies following acute resistance exercise","volume":"6","author":"Townsend","year":"2015","journal-title":"Front. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s12026-012-8297-3","article-title":"The three human monocyte subsets: Implications for health and disease","volume":"53","author":"Wong","year":"2012","journal-title":"Immunol. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.clim.2015.09.012","article-title":"Chemokine receptor expression on monocytes from healthy individuals","volume":"161","author":"Sandblad","year":"2015","journal-title":"Clin. Immunol."},{"key":"ref_23","unstructured":"Thalheimer, W., and Cook, S. How to Calculate Effect Sized from Published Research Articles: A Simplified Methodology. Available online: http:\/\/www.bwgriffin.com\/gsu\/courses\/edur9131\/content\/Effect_Sizes_pdf5.pdf."},{"key":"ref_24","unstructured":"Cohen, J. (1988). Statistical Power Analysis for the Behavioral Sciences, Lawrence Earlbaum Associates. [2nd ed.]."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1123\/ijspp.1.1.50","article-title":"Making meaningful inferences about magnitudes","volume":"1","author":"Batterham","year":"2006","journal-title":"Int. J. Sports Physiol. Perform."},{"key":"ref_26","first-page":"16","article-title":"A spreadsheet for deriving a confidence interval, mechanistic inference and clinical inference from a p value","volume":"11","author":"Hopkins","year":"2007","journal-title":"Sportscience"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e12249","DOI":"10.14814\/phy2.12249","article-title":"Effects of intravenous glucose and lipids on innate immune cell activation in healthy, obese, and type 2 diabetic subjects","volume":"3","author":"Horvath","year":"2015","journal-title":"Physiol. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1089\/jir.2008.0027","article-title":"Monocyte CHemoattractant protein-1 (MCP-1): An overview","volume":"29","author":"Deshmane","year":"2009","journal-title":"J. Interferon Cytokine Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Meijer, K., de Vries, M., Al-Lahham, S., Bruinenberg, M., Weening, D., Dijkstra, M., Kloosterhuis, N., van der Leij, R.J., van der Want, H., and Kroesen, B.J. (2011). Human primary adipocytes exhibit immune cell function: Adipocytes prime inflammation independent of macrophages. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017154"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1006\/cyto.1995.0078","article-title":"Expression of monocyte chemoattractant protein-1 in vascular tissue","volume":"7","author":"Seino","year":"1995","journal-title":"Cytokine"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1161\/01.RES.79.2.286","article-title":"Thrombin potently stimulates cytokine production in human vascular smooth muscle cells but not in mononuclear phagocytes","volume":"79","author":"Kranzhofer","year":"1996","journal-title":"Circ. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cyto.2012.06.018","article-title":"Monocyte chemoattractant protein 1 (MCP-1) in obesity and diabetes","volume":"60","author":"Panee","year":"2012","journal-title":"Cytokine"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1172\/JCI24335","article-title":"CCR2 modulates inflammatory and metabolic effects of high-fat feeding","volume":"116","author":"Weisberg","year":"2006","journal-title":"J. Clin. Investig."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/S0022-2275(20)33509-4","article-title":"Expression of the monocyte chemoattractant protein-1 receptor CCR2 is increased in hypercholesterolemia. Differential effects of plasma lipoproteins on monocyte function","volume":"40","author":"Han","year":"1999","journal-title":"J. Lipid Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1161\/hq0102.102278","article-title":"Local overexpression of monocyte chemoattractant protein-1 at vessel wall induces infiltration of macrophages and formation of atherosclerotic lesion: Synergism with hypercholesterolemia","volume":"22","author":"Namiki","year":"2002","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3032","DOI":"10.1182\/blood.V95.10.3032","article-title":"Chemokine receptors and their role in inflammation and infectious diseases","volume":"95","author":"Murdoch","year":"2000","journal-title":"Blood"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1249\/01.MSS.0000161804.05399.3B","article-title":"Exercise-induced muscle damage, plasma cytokines, and markers of neutrophil activation","volume":"37","author":"Peake","year":"2005","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1007\/s00421-005-0035-2","article-title":"Plasma cytokine changes in relation to exercise intensity and muscle damage","volume":"95","author":"Peake","year":"2005","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1249\/MSS.0000000000000878","article-title":"Monocyte recruitment following high-intensity and high-volume resistance exercise","volume":"48","author":"Wells","year":"2016","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1002\/art.27183","article-title":"Muscle resident macrophages control the immune cell reaction in a mouse model of notexin-induced myoinjury","volume":"62","author":"Brigitte","year":"2010","journal-title":"Arthritis Rheum."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1189\/jlb.0506356","article-title":"MCP-1 deficiency causes altered inflammation with impaired skeletal muscle regeneration","volume":"81","author":"Shireman","year":"2007","journal-title":"J. Leukoc. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0960-9822(99)00268-7","article-title":"Serine phosphorylation and maximal activation of STAT3 during CNTF signaling is mediated by the rapamycin target mTOR","volume":"10","author":"Yokogami","year":"2000","journal-title":"Curr. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"35425","DOI":"10.1074\/jbc.M109.051516","article-title":"Signal transducer and activator of transcription 3 (STAT3) mediates amino acid inhibition of insulin signaling through serine 727 phosphorylation","volume":"284","author":"Kim","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"S47","DOI":"10.1123\/ijsnem.17.s1.s47","article-title":"Role of amino acids and peptides in the molecular signaling in skeletal muscle after resistance exercise","volume":"17","author":"Koopman","year":"2007","journal-title":"Int. J. Sport Nutr. Exerc. Metabl."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1096\/fj.04-2640fje","article-title":"Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle","volume":"19","author":"Cuthbertson","year":"2005","journal-title":"FASEB J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1056\/NEJMra052723","article-title":"The many roles of chemokines and chemokine receptors in inflammation","volume":"354","author":"Charo","year":"2006","journal-title":"N. Engl. J. Med."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Volpe, S., Cameroni, E., Moepps, B., Thelen, S., Apuzzo, T., and Thelen, M. (2012). CCR2 acts as scavenger for CCL2 during monocyte chemotaxis. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0037208"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Kumase, F., Takeuchi, K., Morizane, Y., Suzuki, J., Matsumoto, H., Kataoka, K., Al-Moujahed, A., Maidana, D.E., Miller, J.W., and Vavvas, D.G. (2016). AMPK-activated protein kinase suppresses Ccr2 expression by inhibiting the NF-kappaB pathway in RAW264.7 macrophages. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0147279"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"919","DOI":"10.2527\/jas.2006-342","article-title":"Leucine stimulates mammalian target of rapamycin signaling in C2C12 myoblasts in part through inhibition of adenosine monophosphate-activated protein kinase","volume":"85","author":"Du","year":"2007","journal-title":"J. Anim. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1038\/nri2156","article-title":"Getting to the site of inflammation: The leukocyte adhesion cascade updated","volume":"7","author":"Ley","year":"2007","journal-title":"Nat. Rev. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"R483","DOI":"10.1152\/ajpregu.00542.2013","article-title":"Effects of beta-hydroxy-beta-methylbutyrate free acid and cold water immersion on expression of CR3 and MIP-1beta following resistance exercise","volume":"306","author":"Gonzalez","year":"2014","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1017\/S000711450769936X","article-title":"Amino acids and immune function","volume":"98","author":"Li","year":"2007","journal-title":"Br. J. Nutr."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/8\/7\/409\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:25:20Z","timestamp":1760210720000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/8\/7\/409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,2]]},"references-count":52,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2016,7]]}},"alternative-id":["nu8070409"],"URL":"https:\/\/doi.org\/10.3390\/nu8070409","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,2]]}}}