{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:10:41Z","timestamp":1790104241639,"version":"4.0.1"},"reference-count":48,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2016,10,22]],"date-time":"2016-10-22T00:00:00Z","timestamp":1477094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU VII FP","award":["grant agreement no. 613912"],"award-info":[{"award-number":["grant agreement no. 613912"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Coeliac disease (CD) is associated with alterations of the intestinal microbiota. Although several Bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two Bifidobacterium breve strains (B632 and BR03) to restore the gut microbial balance in coeliac children on a gluten free diet (GFD). Microbial DNA was extracted from faeces of 40 coeliac children before and after probiotic or placebo administration and 16 healthy children (Control group). Sequencing of the amplified V3-V4 hypervariable region of 16S rRNA gene as well as qPCR of Bidobacterium spp., Lactobacillus spp., Bacteroides fragilis group Clostridium sensu stricto and enterobacteria were performed. The comparison between CD subjects and Control group revealed an alteration in the intestinal microbial composition of coeliacs mainly characterized by a reduction of the Firmicutes\/Bacteroidetes ratio, of Actinobacteria and Euryarchaeota. Regarding the effects of the probiotic, an increase of Actinobacteria was found as well as a re-establishment of the physiological Firmicutes\/Bacteroidetes ratio. Therefore, a three-month administration of B. breve strains helps in restoring the healthy percentage of main microbial components.<\/jats:p>","DOI":"10.3390\/nu8100660","type":"journal-article","created":{"date-parts":[[2016,10,24]],"date-time":"2016-10-24T10:46:39Z","timestamp":1477305999000},"page":"660","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":117,"title":["Effect of Bifidobacterium breve on the Intestinal Microbiota of Coeliac Children on a Gluten Free Diet: A Pilot Study"],"prefix":"10.3390","volume":"8","author":[{"given":"Andrea","family":"Quagliariello","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Anthropology, Centre for Genome Biology Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, via Selmi 3, Bologna 40126, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Aloisio","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, University of Bologna, viale Fanin 42, Bologna 40127, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Bozzi Cionci","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, University of Bologna, viale Fanin 42, Bologna 40127, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donata","family":"Luiselli","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Anthropology, Centre for Genome Biology Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, via Selmi 3, Bologna 40126, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0672-2541","authenticated-orcid":false,"given":"Giuseppe","family":"D\u2019Auria","sequence":"additional","affiliation":[{"name":"Sequencing and Bioinformatics Service, Fundaci\u00f3n para el Fomento de la Investigaci\u00f3n Sanitaria y Biom\u00e9dica de la Comunidad Valenciana (FISABIO-Salud P\u00fablica), Valencia 46020, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ll\u00facia","family":"Martinez-Priego","sequence":"additional","affiliation":[{"name":"Sequencing and Bioinformatics Service, Fundaci\u00f3n para el Fomento de la Investigaci\u00f3n Sanitaria y Biom\u00e9dica de la Comunidad Valenciana (FISABIO-Salud P\u00fablica), Valencia 46020, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"P\u00e9rez-Villarroya","sequence":"additional","affiliation":[{"name":"Sequencing and Bioinformatics Service, Fundaci\u00f3n para el Fomento de la Investigaci\u00f3n Sanitaria y Biom\u00e9dica de la Comunidad Valenciana (FISABIO-Salud P\u00fablica), Valencia 46020, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toma\u017e","family":"Langerholc","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, Ho\u010de 2311, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0161a","family":"Primec","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, Ho\u010de 2311, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Du\u0161anka","family":"Mi\u010deti\u0107-Turk","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine, University of Maribor, Taborska ulica 8, Maribor 2000, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Di Gioia","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, University of Bologna, viale Fanin 42, Bologna 40127, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.giec.2012.07.008","article-title":"Presentation of Celiac Disease","volume":"22","author":"Reilly","year":"2012","journal-title":"Gastrointest. 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