{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:06:06Z","timestamp":1791273966526,"version":"4.1.0"},"reference-count":62,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2016,12,7]],"date-time":"2016-12-07T00:00:00Z","timestamp":1481068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Leuven","award":["Methusalem grant"],"award-info":[{"award-number":["Methusalem grant"]}]},{"name":"Agency for Innovation by Science and Technology","award":["IWT scholarship"],"award-info":[{"award-number":["IWT scholarship"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Carbohydrate administration decreases plasma levels of the \u2018hunger hormone\u2019 ghrelin. The ghrelin cell is co-localized with the sweet taste receptor subunit, TAS1R3, and the gustatory G-protein, gustducin, both involved in the sensing of sweeteners by entero-endocrine cells. This study investigated the role of gustducin-mediated sweet taste receptor signaling on ghrelin secretion in a gastric ghrelinoma cell line, tissue segments and mice. The monosaccharide d-glucose and low-intensity sweetener oligofructose (OFS) decreased (p &lt; 0.001) ghrelin secretion while the high-intensity sweetener sucralose increased (p &lt; 0.001) ghrelin secretion in vitro. These effects were not mediated via the sweet taste receptor or glucose transporters (the sodium-dependent glucose cotransporter SGLT-1 and GLUT2). The effect of these compounds was mimicked ex vivo in gastric and jejunal segments from both wild type (WT) and \u03b1-gustducin knockout (\u03b1-gust\u2212\/\u2212) mice. In vivo, the sensing of d-glucose was polarized since intragastric but not intravenous administration of d-glucose decreased (p &lt; 0.05) ghrelin levels in an \u03b1-gustducin independent manner which involved inhibition of duodenal ghrelin release. In contrast, neither OFS nor sucralose affected ghrelin secretion in vivo. In conclusion, \u03b1-gustducin-mediated sweet taste receptor signaling does not play a functional role in the sensing of carbohydrates, or low- or high-intensity sweeteners by the ghrelin cell.<\/jats:p>","DOI":"10.3390\/nu8120795","type":"journal-article","created":{"date-parts":[[2016,12,8]],"date-time":"2016-12-08T10:39:01Z","timestamp":1481193541000},"page":"795","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The Sweetener-Sensing Mechanisms of the Ghrelin Cell"],"prefix":"10.3390","volume":"8","author":[{"given":"Sandra","family":"Steensels","sequence":"first","affiliation":[{"name":"Gut Peptide Lab, Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven\u2014KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurien","family":"Vancleef","sequence":"additional","affiliation":[{"name":"Gut Peptide Lab, Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven\u2014KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge","family":"Depoortere","sequence":"additional","affiliation":[{"name":"Gut Peptide Lab, Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven\u2014KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"NCD Risk Factor Collaboration (NCD-RisC) (2016). 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