{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:04:48Z","timestamp":1787241888458,"version":"build-2736575974"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T00:00:00Z","timestamp":1484870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Wheat bran (WB) is a constituent of whole grain products with beneficial effects for human health. Within the human colon, such insoluble particles may be colonized by specific microbial teams which can stimulate cross-feeding, leading to a more efficient carbohydrate fermentation and an increased butyrate production. We investigated the extent to which WB fractions with different properties affect the fermentation of other carbohydrates in the colon. Ten healthy subjects performed four test days, during which they consumed a standard breakfast supplemented with 10 g 13C-inulin. A total of 20 g of a WB fraction (unmodified WB, wheat bran with a reduced particle size (WB RPS), or de-starched pericarp-enriched wheat bran (PE WB)) was also added to the breakfast, except for one test day, which served as a control. Blood samples were collected at regular time points for 14 h, in order to measure 13C-labeled short-chain fatty acid (SCFA; acetate, propionate and butyrate) concentrations. Fermentation of 13C-inulin resulted in increased plasma SCFA for about 8 h, suggesting that a sustained increase in plasma SCFA can be achieved by administering a moderate dose of carbohydrates, three times per day. However, the addition of a single dose of a WB fraction did not further increase the 13C-SCFA concentrations in plasma, nor did it stimulate cross-feeding (Wilcoxon signed ranks test).<\/jats:p>","DOI":"10.3390\/nu9010083","type":"journal-article","created":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T10:10:12Z","timestamp":1484907012000},"page":"83","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Wheat Bran Does Not Affect Postprandial Plasma Short-Chain Fatty Acids from 13C-inulin Fermentation in Healthy Subjects"],"prefix":"10.3390","volume":"9","author":[{"given":"Lise","family":"Deroover","sequence":"first","affiliation":[{"name":"Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6671-3121","authenticated-orcid":false,"given":"Joran","family":"Verspreet","sequence":"additional","affiliation":[{"name":"Centre for Food and Microbial Technology, KU Leuven, Leuven 3000, Belgium"},{"name":"Leuven Food Science and Nutrition Research Centre, KU Leuven, Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Luypaerts","sequence":"additional","affiliation":[{"name":"Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greet","family":"Vandermeulen","sequence":"additional","affiliation":[{"name":"Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1203-9063","authenticated-orcid":false,"given":"Christophe","family":"Courtin","sequence":"additional","affiliation":[{"name":"Centre for Food and Microbial Technology, KU Leuven, Leuven 3000, Belgium"},{"name":"Leuven Food Science and Nutrition Research Centre, KU Leuven, Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5352-7565","authenticated-orcid":false,"given":"Kristin","family":"Verbeke","sequence":"additional","affiliation":[{"name":"Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium"},{"name":"Leuven Food Science and Nutrition Research Centre, KU Leuven, Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1194\/jlr.R036012","article-title":"The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism","volume":"54","author":"Groen","year":"2013","journal-title":"J. Lipid Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"50","DOI":"10.5740\/jaoacint.SGE_Macfarlane","article-title":"Bacteria, colonic fermentation, and gastrointestinal health","volume":"95","author":"Macfarlane","year":"2012","journal-title":"J. AOAC Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/j.1365-2036.2007.03562.x","article-title":"Review article: The role of butyrate on colonic function","volume":"27","author":"Hamer","year":"2008","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"S120","DOI":"10.1097\/MCG.0b013e31822fecfe","article-title":"Fermentation in the human large intestine: Its physiologic consequences and the potential contribution of prebiotics","volume":"45","author":"Macfarlane","year":"2011","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/nrendo.2015.128","article-title":"Short-chain fatty acids in control of body weight and insulin sensitivity","volume":"11","author":"Canfora","year":"2015","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5092","DOI":"10.1210\/en.2005-0545","article-title":"Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43","volume":"146","author":"Hong","year":"2005","journal-title":"Endocrinology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1038\/mi.2014.44","article-title":"Short-chain fatty acids induce both effector and regulatory T cells by suppression of histone deacetylases and regulation of the mtor-s6k pathway","volume":"8","author":"Park","year":"2015","journal-title":"Mucosal. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1126\/science.1241165","article-title":"The microbial metabolites, short-chain fatty acids, regulate colonic Treg cell homeostasis","volume":"341","author":"Smith","year":"2013","journal-title":"Science"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s11938-002-0054-6","article-title":"Probiotics and functional foods in gastrointestinal disorders","volume":"5","author":"Floch","year":"2002","journal-title":"Curr. Treat. Opt. Gastroenterol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1136\/gutjnl-2012-304240","article-title":"Butyrate-producing bacteria as pharmabiotics for inflammatory bowel disease","volume":"62","author":"Marteau","year":"2013","journal-title":"Gut"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1136\/gutjnl-2013-305293","article-title":"Progress towards butyrate-producing pharmabiotics: Butyricicoccus pullicaecorum capsule and efficacy in TNBS models in comparison with therapeutics","volume":"63","author":"Eeckhaut","year":"2014","journal-title":"Gut"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1136\/gutjnl-2012-303611","article-title":"Butyricicoccus pullicaecorum in inflammatory bowel disease","volume":"62","author":"Eeckhaut","year":"2013","journal-title":"Gut"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.mib.2013.06.003","article-title":"Faecalibacterium prausnitzii and human intestinal health","volume":"16","author":"Miquel","year":"2013","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_14","first-page":"1","article-title":"Dietary prebiotics: Current status and new definition","volume":"7","author":"Gibson","year":"2010","journal-title":"Food Sci. Technol. Bull."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1006\/anae.1999.0318","article-title":"Effect of oligosaccharides and fibre substitutes on short-chain fatty acid production by human faecal microflora","volume":"6","author":"Davies","year":"2000","journal-title":"Anaerobe"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0924-2244(02)00131-0","article-title":"Resistant starch and \u201cthe butyrate revolution\u201d","volume":"13","author":"Brouns","year":"2002","journal-title":"Trends Food Sci. Technol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.ijfoodmicro.2016.05.015","article-title":"Bifidobacterial inulin-type fructan degradation capacity determines cross-feeding interactions between bifidobacteria and faecalibacterium prausnitzii","volume":"231","author":"Moens","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7767","DOI":"10.1128\/AEM.02089-15","article-title":"Mutual cross-feeding interactions between bifidobacterium longum subsp. Longum NCC2705 and eubacterium rectale ATCC 33656 explain the bifidogenic and butyrogenic effects of arabinoxylan oligosaccharides","volume":"81","author":"Riviere","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/j.1462-2920.2006.01186.x","article-title":"Selective colonization of insoluble substrates by human faecal bacteria","volume":"9","author":"Leitch","year":"2007","journal-title":"Environ. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0021-9673(99)01064-X","article-title":"Determination of reducing end sugar residues in oligo- and polysaccharides by gas-liquid chromatography","volume":"866","author":"Courtin","year":"2000","journal-title":"J. Chromatogr. A"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.lwt.2013.03.015","article-title":"Single run hplc separation coupled to evaporative light scattering detection unravels wheat flour endogenous lipid redistribution during bread dough making","volume":"53","author":"Gerits","year":"2013","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.foodchem.2015.01.117","article-title":"Study of hydration properties of wheat bran as a function of particle size","volume":"179","author":"Jacobs","year":"2015","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1002\/jsfa.2470","article-title":"Large-scale production and characterisation of wheat bran arabinoxylooligosaccharides","volume":"86","author":"Swennen","year":"2006","journal-title":"J. Sci. Food Agric."},{"key":"ref_24","unstructured":"Urbaniak, G.U., and Plous, S. Research Randomizer. Available online: http:\/\/www.randomizer.org."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"8916","DOI":"10.3390\/nu7115440","article-title":"Quantification of in vivo colonic short chain fatty acid production from inulin","volume":"7","author":"Boets","year":"2015","journal-title":"Nutrients"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1365-2036.2005.02323.x","article-title":"In vivo evaluation of a colonic delivery system using isotope techniques","volume":"21","author":"Verbeke","year":"2005","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jchromb.2006.09.027","article-title":"Determination of short-chain fatty acids in serum by hollow fiber supported liquid membrane extraction coupled with gas chromatography","volume":"846","author":"Zhao","year":"2007","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.trsl.2012.10.007","article-title":"Short chain fatty acids and their receptors: New metabolic targets","volume":"161","author":"Layden","year":"2013","journal-title":"Transl. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S1388-1981(01)00117-2","article-title":"Production of acetate in the liver and its utilization in peripheral tissues","volume":"1532","author":"Yamashita","year":"2001","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1053\/j.gastro.2007.11.059","article-title":"Microbial influences in inflammatory bowel diseases","volume":"134","author":"Sartor","year":"2008","journal-title":"Gastroenterology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1128\/AEM.72.2.1006-1012.2006","article-title":"In vitro kinetic analysis of oligofructose consumption by Bacteroides and Bifidobacterium spp. Indicates different degradation mechanisms","volume":"72","author":"Makras","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1046\/j.1365-2036.2003.01750.x","article-title":"Inulin is an ideal substrate for a hydrogen breath test to measure the orocaecal transit time","volume":"18","author":"Geboes","year":"2003","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1111\/j.1365-2362.2007.01862.x","article-title":"The inulin hydrogen breath test accurately reflects orocaecal transit time","volume":"37","author":"Schneider","year":"2007","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1136\/gut.45.6.840","article-title":"Wheat bran affects the site of fermentation of resistant starch and luminal indexes related to colon cancer risk: A study in pigs","volume":"45","author":"Govers","year":"1999","journal-title":"Gut"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1093\/ajcn\/79.6.1020","article-title":"Combining wheat bran with resistant starch has more beneficial effects on fecal indexes than does wheat bran alone","volume":"79","author":"Muir","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"8952","DOI":"10.3748\/wjg.v21.i29.8952","article-title":"Effects of cereal fiber on bowel function: A systematic review of intervention trials","volume":"21","author":"Miller","year":"2015","journal-title":"World J. Gastroenterol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1136\/gut.41.2.245","article-title":"Increasing butyrate concentration in the distal colon by accelerating intestinal transit","volume":"41","author":"Lewis","year":"1997","journal-title":"Gut"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1007\/s002489900072","article-title":"Validation of a three-stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human colon","volume":"35","author":"Macfarlane","year":"1998","journal-title":"Microb. Ecol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"16093","DOI":"10.1038\/nmicrobiol.2016.93","article-title":"Colonic transit time is related to bacterial metabolism and mucosal turnover in the gut","volume":"1","author":"Roager","year":"2016","journal-title":"Nat. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/j.clnu.2009.05.011","article-title":"Short chain fatty acids exchange across the gut and liver in humans measured at surgery","volume":"28","author":"Bloemen","year":"2009","journal-title":"Clin. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1113\/JP272613","article-title":"Systemic availability and metabolism of colonic-derived short-chain fatty acids in healthy subjects\u2014A stable isotope study","volume":"595","author":"Boets","year":"2017","journal-title":"J. Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1136\/gut.33.9.1249","article-title":"Portal and peripheral blood short chain fatty acid concentrations after caecal lactulose instillation at surgery","volume":"33","author":"Peters","year":"1992","journal-title":"Gut"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/9\/1\/83\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:26:40Z","timestamp":1760207200000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/9\/1\/83"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,20]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,1]]}},"alternative-id":["nu9010083"],"URL":"https:\/\/doi.org\/10.3390\/nu9010083","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,20]]}}}