{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:28:14Z","timestamp":1780493294422,"version":"3.54.1"},"reference-count":42,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2017,3,11]],"date-time":"2017-03-11T00:00:00Z","timestamp":1489190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Previous investigations have determined that some individuals have minimal or even ergolytic performance effects after caffeine ingestion. The aim of this study was to analyze the influence of the genetic variations of the CYP1A2 gene on the performance enhancement effects of ingesting a moderate dose of caffeine. In a double-blind randomized experimental design, 21 healthy active participants (29.3 \u00b1 7.7 years) ingested 3 mg of caffeine per kg of body mass or a placebo in testing sessions separated by one week. Performance in the 30 s Wingate test, visual attention, and side effects were evaluated. DNA was obtained from whole blood samples and the CYP1A2 polymorphism was analyzed (rs762551). We obtained two groups: AA homozygotes (n = 5) and C-allele carriers (n = 16). Caffeine ingestion increased peak power (682 \u00b1 140 vs. 667 \u00b1 137 W; p = 0.008) and mean power during the Wingate test (527 \u00b1 111 vs. 518 \u00b1 111 W; p &lt; 0.001) with no differences between AA homozygotes and C-allele carriers (p &gt; 0.05). Reaction times were similar between caffeine and placebo conditions (276 \u00b1 31 vs. 269 \u00b1 71 milliseconds; p = 0.681) with no differences between AA homozygotes and C-allele carriers. However, 31.3% of the C-allele carriers reported increased nervousness after caffeine ingestion, while none of the AA homozygotes perceived this side effect. Genetic variations of the CYP1A2 polymorphism did not affect the ergogenic effects and drawbacks derived from the ingestion of a moderate dose of caffeine.<\/jats:p>","DOI":"10.3390\/nu9030269","type":"journal-article","created":{"date-parts":[[2017,3,13]],"date-time":"2017-03-13T10:26:18Z","timestamp":1489400778000},"page":"269","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["CYP1A2 Genotype Variations Do Not Modify the Benefits and Drawbacks of Caffeine during Exercise: A Pilot Study"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4153-5100","authenticated-orcid":false,"given":"Juan","family":"Salinero","sequence":"first","affiliation":[{"name":"Exercise Physiology Laboratory, Camilo Jos\u00e9 Cela University, Madrid 28692, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5516-4852","authenticated-orcid":false,"given":"Beatriz","family":"Lara","sequence":"additional","affiliation":[{"name":"Exercise Physiology Laboratory, Camilo Jos\u00e9 Cela University, Madrid 28692, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Ruiz-Vicente","sequence":"additional","affiliation":[{"name":"Exercise Physiology Laboratory, Camilo Jos\u00e9 Cela University, Madrid 28692, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Areces","sequence":"additional","affiliation":[{"name":"Exercise Physiology Laboratory, Camilo Jos\u00e9 Cela University, Madrid 28692, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Puente-Torres","sequence":"additional","affiliation":[{"name":"Exercise Physiology Laboratory, Camilo Jos\u00e9 Cela University, Madrid 28692, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar","family":"Gallo-Salazar","sequence":"additional","affiliation":[{"name":"Exercise Physiology Laboratory, Camilo Jos\u00e9 Cela University, Madrid 28692, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teodoro","family":"Pascual","sequence":"additional","affiliation":[{"name":"Exercise Physiology Laboratory, Camilo Jos\u00e9 Cela University, Madrid 28692, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5785-984X","authenticated-orcid":false,"given":"Juan","family":"Del Coso","sequence":"additional","affiliation":[{"name":"Exercise Physiology Laboratory, Camilo Jos\u00e9 Cela University, Madrid 28692, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1550-2783-7-5","article-title":"International society of sports nutrition position stand: Caffeine and performance","volume":"7","author":"Goldstein","year":"2010","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1017\/S0007114515002573","article-title":"Acute consumption of a caffeinated energy drink enhances aspects of performance in sprint swimmers","volume":"114","author":"Lara","year":"2015","journal-title":"Br. J. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1139\/apnm-2012-0339","article-title":"Caffeine-containing energy drink improves physical performance of elite rugby players during a simulated match","volume":"38","author":"Ramirez","year":"2013","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1007\/s00726-014-1709-z","article-title":"Caffeine-containing energy drink improves physical performance in female soccer players","volume":"46","author":"Lara","year":"2014","journal-title":"Amino Acids"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1249\/MSS.0000000000000455","article-title":"Caffeinated energy drinks improve volleyball performance in elite female players","volume":"47","author":"Salinero","year":"2015","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.jsams.2013.07.004","article-title":"Effect of caffeine on cycling time-trial performance in the heat","volume":"17","author":"Pitchford","year":"2014","journal-title":"J. Sci. Med. Sport"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Hodgson, A.B., Randell, R.K., and Jeukendrup, A.E. (2013). The metabolic and performance effects of caffeine compared to coffee during endurance exercise. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0059561"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1550-2783-9-21","article-title":"Dose response effects of a caffeine-containing energy drink on muscle performance: A repeated measures design","volume":"9","author":"Salinero","year":"2012","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1080\/17461391.2011.635811","article-title":"Acute caffeine ingestion enhances strength performance and reduces perceived exertion and muscle pain perception during resistance exercise","volume":"13","author":"Duncan","year":"2013","journal-title":"Eur. J. Sport Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.pbb.2012.07.004","article-title":"Differential cognitive effects of energy drink ingredients: Caffeine, taurine, and glucose","volume":"102","author":"Giles","year":"2012","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1007\/s00213-004-2104-3","article-title":"Cognitive and mood improvements of caffeine in habitual consumers and habitual non-consumers of caffeine","volume":"179","author":"Haskell","year":"2005","journal-title":"Psychopharmacology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.euroneuro.2015.03.001","article-title":"Genetic determinants of cognitive responses to caffeine drinking identified from a double-blind, randomized, controlled trial","volume":"25","author":"Renda","year":"2015","journal-title":"Eur. Neuropsychopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1007\/s00213-002-1217-9","article-title":"Effects of caffeine, sleep loss, and stress on cognitive performance and mood during US Navy seal training. Sea-air-land","volume":"164","author":"Lieberman","year":"2002","journal-title":"Psychopharmacology"},{"key":"ref_14","first-page":"647","article-title":"Caffeine maintains vigilance and improves run times during night operations for special forces","volume":"76","author":"McLellan","year":"2005","journal-title":"Aviat Space Environ. Med."},{"key":"ref_15","first-page":"871","article-title":"Caffeine effects on physical and cognitive performance during sustained operations","volume":"78","author":"McLellan","year":"2007","journal-title":"Aviat Space Environ. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1249\/MSS.0b013e31817bb8b7","article-title":"Caffeine improves physical and cognitive performance during exhaustive exercise","volume":"40","author":"Hogervorst","year":"2008","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1017\/S0007114514002189","article-title":"The use of energy drinks in sport: Perceived ergogenicity and side effects in male and female athletes","volume":"112","author":"Salinero","year":"2014","journal-title":"Br. J. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000322696","article-title":"Caffeine and creatine use in sport","volume":"57","author":"Tarnopolsky","year":"2010","journal-title":"Ann. Nutr. Metab."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"813","DOI":"10.2165\/11317770-000000000-00000","article-title":"Caffeine and anaerobic performance: Ergogenic value and mechanisms of action","volume":"39","author":"Davis","year":"2009","journal-title":"Sports Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1080\/1040-830491379245","article-title":"Caffeine use in sports, pharmacokinetics in man, and cellular mechanisms of action","volume":"45","author":"Magkos","year":"2005","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1249\/MSS.0b013e3181b6668b","article-title":"Dose response of caffeine on 2000-m rowing performance","volume":"42","author":"Skinner","year":"2010","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1152\/japplphysiol.00937.2004","article-title":"Caffeine increases time to fatigue by maintaining force and not by altering firing rates during submaximal isometric contractions","volume":"99","author":"Meyers","year":"2005","journal-title":"J. Appl. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1097\/00005768-200211000-00015","article-title":"Caffeine is ergogenic after supplementation of oral creatine monohydrate","volume":"34","author":"Doherty","year":"2002","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"667","DOI":"10.2174\/1389200211209050667","article-title":"Measurement of CYP1A2 activity: A focus on caffeine as a probe","volume":"13","author":"Perera","year":"2012","journal-title":"Curr. Drug Metab."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s00228-007-0288-2","article-title":"Comparisons of CYP1A2 genetic polymorphisms, enzyme activity and the genotype-phenotype relationship in Swedes and Koreans","volume":"63","author":"Ghotbi","year":"2007","journal-title":"Eur. J. Clin. Pharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1007\/s00228-010-0823-4","article-title":"Induction of CYP1A2 by heavy coffee consumption is associated with the CYP1A2 -163C>A polymorphism","volume":"66","author":"Djordjevic","year":"2010","journal-title":"Eur. J. Clin. Pharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1550-2783-9-7","article-title":"The influence of a CYP1A2 polymorphism on the ergogenic effects of caffeine","volume":"9","author":"Womack","year":"2012","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/sms.12501","article-title":"Caffeine and 3-km cycling performance: Effects of mouth rinsing, genotype, and time of day","volume":"26","author":"Pataky","year":"2016","journal-title":"Scand. J. Med. Sci. Sports"},{"key":"ref_29","first-page":"1","article-title":"The effects of a polymorphism in the cytochrome p450 CYP1A2 gene on performance enhancement with caffeine in recreational cyclists","volume":"6","author":"Algrain","year":"2015","journal-title":"J. Caffeine Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1123\/ijsnem.12.2.189","article-title":"Caffeine, body fluid-electrolyte balance, and exercise performance","volume":"12","author":"Armstrong","year":"2002","journal-title":"Int. J. Sport Nutr. Exerc. Metab."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Attia, A., Hachana, Y., Chaabene, H., Gaddour, A., Neji, Z., Shephard, R.J., and Chelly, M.S. (2014). Reliability and validity of a 20-s alternative to the wingate anaerobic test in team sport male athletes. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0114444"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"381","DOI":"10.2165\/00007256-198704060-00001","article-title":"The wingate anaerobic test. An update on methodology, reliability and validity","volume":"4","year":"1987","journal-title":"Sports Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1055\/s-2007-1024710","article-title":"Effects of caffeine ingestion on performance and anaerobic metabolism during the wingate test","volume":"12","author":"Collomp","year":"1991","journal-title":"Int. J. Sports Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1152\/jappl.1998.85.4.1502","article-title":"Caffeine, performance, and metabolism during repeated wingate exercise tests","volume":"85","author":"Greer","year":"1998","journal-title":"J. Appl. Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1007\/BF00210835","article-title":"The effects of low doses of caffeine on human performance and mood","volume":"92","author":"Lieberman","year":"1987","journal-title":"Psychopharmacology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"637","DOI":"10.3390\/nu6020637","article-title":"Caffeine reduces reaction time and improves performance in simulated-contest of taekwondo","volume":"6","author":"Santos","year":"2014","journal-title":"Nutrients"},{"key":"ref_37","first-page":"1153","article-title":"Effects of caffeine on simple reaction time and movement time","volume":"58","author":"Jacobson","year":"1987","journal-title":"Aviat Space Environ. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1123\/ijsnem.16.5.528","article-title":"Physiological and cognitive responses to caffeine during repeated, high-intensity exercise","volume":"16","author":"Crowe","year":"2006","journal-title":"Int. J. Sport Nutr. Exerc. Metab."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1007\/s00726-013-1473-5","article-title":"Caffeine-containing energy drink improves sprint performance during an international rugby sevens competition","volume":"44","author":"Portillo","year":"2013","journal-title":"Amino Acids"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1038\/sj.clpt.6100102","article-title":"A genetic variation in the adenosine A2A receptor gene (adora2a) contributes to individual sensitivity to caffeine effects on sleep","volume":"81","author":"Retey","year":"2007","journal-title":"Clin. Pharmacol. Ther."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1038\/sj.npp.1300232","article-title":"Association between A2A receptor gene polymorphisms and caffeine-induced anxiety","volume":"28","author":"Alsene","year":"2003","journal-title":"Neuropsychopharmacology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/S0009-9236(98)90105-9","article-title":"Quantitation of three-month intraindividual variability and influence of sex and menstrual cycle phase on CYP1A2, N-acetyltransferase-2, and xanthine oxidase activity determined with caffeine phenotyping","volume":"63","author":"Kashuba","year":"1998","journal-title":"Clin. Pharmacol. Ther."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/9\/3\/269\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:30:16Z","timestamp":1760207416000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/9\/3\/269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,11]]},"references-count":42,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2017,3]]}},"alternative-id":["nu9030269"],"URL":"https:\/\/doi.org\/10.3390\/nu9030269","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,11]]}}}