{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T19:57:24Z","timestamp":1772308644185,"version":"3.50.1"},"reference-count":80,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2017,9,21]],"date-time":"2017-09-21T00:00:00Z","timestamp":1505952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003952","name":"Ministry for Food, Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","award":["313024-03-2-HD040"],"award-info":[{"award-number":["313024-03-2-HD040"]}],"id":[{"id":"10.13039\/501100003952","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Phyllodulcin is a natural sweetener found in Hydrangea macrophylla var. thunbergii. This study investigated whether phyllodulcin could improve metabolic abnormalities in high-fat diet (HFD)-induced obese mice. Animals were fed a 60% HFD for 6 weeks to induce obesity, followed by 7 weeks of supplementation with phyllodulcin (20 or 40 mg\/kg body weight (b.w.)\/day). Stevioside (40 mg\/kg b.w.\/day) was used as a positive control. Phyllodulcin supplementation reduced subcutaneous fat mass, levels of plasma lipids, triglycerides, total cholesterol, and low-density lipoprotein cholesterol and improved the levels of leptin, adiponectin, and fasting blood glucose. In subcutaneous fat tissues, supplementation with stevioside or phyllodulcin significantly decreased mRNA expression of lipogenesis-related genes, including CCAAT\/enhancer-binding protein \u03b1 (C\/EBP\u03b1), peroxisome proliferator activated receptor \u03b3 (PPAR\u03b3), and sterol regulatory element-binding protein-1C (SREBP-1c) compared to the high-fat group. Phyllodulcin supplementation significantly increased the expression of fat browning-related genes, including PR domain containing 16 (Prdm16), uncoupling protein 1 (UCP1), and peroxisome proliferator-activated receptor \u03b3 coactivator 1-\u03b1 (PGC-1\u03b1), compared to the high-fat group. Hypothalamic brain-derived neurotrophic factor-tropomyosin receptor kinase B (BDNF-TrkB) signaling was upregulated by phyllodulcin supplementation. In conclusion, phyllodulcin is a potential sweetener that could be used to combat obesity by regulating levels of leptin, fat browning-related genes, and hypothalamic BDNF-TrkB signaling.<\/jats:p>","DOI":"10.3390\/nu9101049","type":"journal-article","created":{"date-parts":[[2017,9,21]],"date-time":"2017-09-21T12:17:40Z","timestamp":1505996260000},"page":"1049","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Phyllodulcin, a Natural Sweetener, Regulates Obesity-Related Metabolic Changes and Fat Browning-Related Genes of Subcutaneous White Adipose Tissue in High-Fat Diet-Induced Obese Mice"],"prefix":"10.3390","volume":"9","author":[{"given":"Eunju","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo-Min","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang-Ho","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,9,21]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2016). 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