{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T02:53:49Z","timestamp":1784429629965,"version":"3.55.0"},"reference-count":26,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Citrus fruit and olive leaves are a source of bioactive compounds such as biophenols which have been shown to ameliorate obesity-related conditions through their anti-hyperlipidemic and anti-inflammatory effect, and by regulating lipoproteins and cholesterol body levels. Citrolive\u2122 is a commercial extract which is obtained from the combination of both citrus fruit and olive leaf extracts; hence, it is hypothesised that Citrolive\u2122 may moderate metabolic disorders that are related to obesity and their complications. Initially, an in vitro study of the inhibition of pancreatic lipase activity was made, however, no effect was found. Both preliminary and long-term evaluations of Citrolive\u2122 on lipid metabolism were conducted in an animal model using Wistar rats. In the preliminary in vivo screening, Citrolive\u2122 was tested on postprandial plasma triglyceride level after the administration of an oil emulsion, and a significant reduction in postprandial triacylglycerol (TAG) levels was observed. In the long-term study, Citrolive\u2122 was administered for 60 days on Wistar rats that were fed a high-fat diet. During the study, several associated lipid metabolism indicators were analysed in blood and faeces. At the end of the experiment, the livers were removed and weighed for group comparison. Citrolive\u2122 treatment significantly reduced the liver-to-body-weight ratio, as supported by reduced plasma transaminases compared with control, but insignificantly reduced plasma low density lipoprotein (LDL) and postprandial TAG plasma levels. In addition, faecal analysis showed that the treatment significantly increased total cholesterol excretion. On the other hand, no effect was found on faecal TAG and pancreatic lipase in vitro. In conclusion, treatment ameliorates liver inflammation symptoms that are worsened by the effects of high fat diet.<\/jats:p>","DOI":"10.3390\/nu9101062","type":"journal-article","created":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T16:14:45Z","timestamp":1506442485000},"page":"1062","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The Effect of Consumption of Citrus Fruit and Olive Leaf Extract on Lipid Metabolism"],"prefix":"10.3390","volume":"9","author":[{"given":"Nicola","family":"Merola","sequence":"first","affiliation":[{"name":"Department of Food Technology, Nutrition and Food Science, Veterinary Faculty University of Murcia, Campus de Espinardo, 30100 Espinardo, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juli\u00e1n","family":"Castillo","sequence":"additional","affiliation":[{"name":"Research and Development Department of Nutrafur-Frutarom Group, Camino Viejo de Pliego s\/n, 80320 Alcantarilla, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Obdulio","family":"Benavente-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Research and Development Department of Nutrafur-Frutarom Group, Camino Viejo de Pliego s\/n, 80320 Alcantarilla, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8894-4481","authenticated-orcid":false,"given":"Gaspar","family":"Ros","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Nutrition and Food Science, Veterinary Faculty University of Murcia, Campus de Espinardo, 30100 Espinardo, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2349-2899","authenticated-orcid":false,"given":"Gema","family":"Nieto","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Nutrition and Food Science, Veterinary Faculty University of Murcia, Campus de Espinardo, 30100 Espinardo, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/j.psc.2011.08.005","article-title":"Obesity: Overview of an Epidemic","volume":"34","author":"Mitchell","year":"2011","journal-title":"Psychiatr. 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