{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:19:56Z","timestamp":1760059196690,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nursing Reports"],"abstract":"<jats:p>Background\/Objectives: The COVID-19 pandemic, caused by SARS-CoV-2, required the rapid development of diagnostic tests. SARS-CoV-2, part of the betacoronavirus genus, shares characteristics with SARS-CoV-1, including its ability to survive on surfaces, facilitating the spread of the infection. This study analyzes the technique of nasopharyngeal secretion collection for SARS-CoV-2 diagnosis and compares the accuracy of rapid antigen and molecular tests. Methods: This study had two components: study A assessed the healthcare personnel training in collecting nasopharyngeal secretions and the discomfort associated with applying a questionnaire. Study B compared rapid antigen test accuracy with RT-PCR among children, through a retrospective analysis. The data were statistically analyzed to assess compliance with the testing protocols. Results: In study A, 88 healthcare workers achieved an average compliance score of 7.60 out of 10 regarding the collection procedure. Over 70% of participants correctly followed the fundamental steps of the procedure. Many patients who underwent sample collection reported pain and symptoms such as coughing or sneezing. In study B, 198 pediatric patients were tested using rapid antigen tests, collected simultaneously with RT-PCR. The rapid tests showed a 50% sensitivity and 97.5% specificity. Conclusions: This study indicates that nasopharyngeal specimen collection techniques are based on international recommendations, but improvements could be made to reduce discomfort. Rapid antigen tests are helpful for screening due to their high specificity and negative predictive value. Continuous healthcare personnel training and the monitoring of diagnostic techniques remain essential in managing SARS-CoV-2 and other viral infections.<\/jats:p>","DOI":"10.3390\/nursrep15060196","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T05:09:12Z","timestamp":1748840952000},"page":"196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Practical Challenges in the Diagnosis of SARS-CoV-2 Infection in Children"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4635-240X","authenticated-orcid":false,"given":"Alina Petronela","family":"Bouari-Cobli\u0219an","sequence":"first","affiliation":[{"name":"General Nursing Discipline, Faculty of Nursing and Health Sciences, Iuliu Ha\u021bieganu University of Medicine and Pharmacy, 400124 Cluj-Napoca, Romania"},{"name":"3rd Pediatric Clinic, Children\u2019s Emergency Clinical Hospital, 400315 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Felicia","family":"Pop","sequence":"additional","affiliation":[{"name":"General Nursing Discipline, Faculty of Nursing and Health Sciences, Iuliu Ha\u021bieganu University of Medicine and Pharmacy, 400124 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1405-9519","authenticated-orcid":false,"given":"Valentina","family":"Sas","sequence":"additional","affiliation":[{"name":"3rd Pediatric Clinic, Children\u2019s Emergency Clinical Hospital, 400315 Cluj-Napoca, Romania"},{"name":"Third Pediatric Discipline, Department 8, Mother and Child, Faculty of Medicine, Iuliu Ha\u021bieganu University of Medicine and Pharmacy, 400124 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adina Georgiana","family":"Borc\u0103u","sequence":"additional","affiliation":[{"name":"Ophthalmology Network Dr. Holho\u0219, 510009 Alba Iulia, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teodora Irina","family":"Bonci","sequence":"additional","affiliation":[{"name":"Pathophysiology, Department of Morphofunctional Sciences, Faculty of Medicine, Iuliu Ha\u021bieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7427-0191","authenticated-orcid":false,"given":"Paraschiva","family":"Chereche\u0219-Pan\u021ba","sequence":"additional","affiliation":[{"name":"3rd Pediatric Clinic, Children\u2019s Emergency Clinical Hospital, 400315 Cluj-Napoca, Romania"},{"name":"Third Pediatric Discipline, Department 8, Mother and Child, Faculty of Medicine, Iuliu Ha\u021bieganu University of Medicine and Pharmacy, 400124 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1038\/s41591-021-01381-y","article-title":"Health Systems Resilience in Managing the COVID-19 Pandemic: Lessons from 28 Countries","volume":"27","author":"Haldane","year":"2021","journal-title":"Nat. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.bj.2020.04.007","article-title":"COVID-19: The First Documented Coronavirus Pandemic in History","volume":"43","author":"Liu","year":"2020","journal-title":"Biomed. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/nrmicro2147","article-title":"Coronaviruses Post-SARS: Update on Replication and Pathogenesis","volume":"7","author":"Perlman","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s12985-019-1182-0","article-title":"Coronavirus Envelope Protein: Current Knowledge","volume":"16","author":"Schoeman","year":"2019","journal-title":"Virol. J."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Vallamkondu, J., John, A., Wani, W.Y., Ramadevi, S.P., Jella, K.K., Reddy, P.H., and Kandimalla, R. (2020). SARS-CoV-2 Pathophysiology and Assessment of Coronaviruses in CNS Diseases with a Focus on Therapeutic Targets. Biochim. Biophys. Acta Mol. Basis Dis., 1866.","DOI":"10.1016\/j.bbadis.2020.165889"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"549","DOI":"10.3906\/sag-2004-127","article-title":"Coronaviruses and SARS-CoV-2","volume":"50","year":"2020","journal-title":"Turk. J. Med. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Payne, S. (2017). Family Coronaviridae. Viruses, 149\u2013158.","DOI":"10.1016\/B978-0-12-803109-4.00017-9"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"119","DOI":"10.3345\/cep.2020.00493","article-title":"Epidemiology, Virology, and Clinical Features of Severe Acute Respiratory Syndrome -Coronavirus-2 (SARS-CoV-2; Coronavirus Disease-19)","volume":"63","author":"Park","year":"2020","journal-title":"Clin. Exp. Pediatr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1056\/NEJMc2004973","article-title":"Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1","volume":"382","author":"Bushmaker","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"551","DOI":"10.5694\/mja2.52151","article-title":"COVID-19 Rapid Antigen Tests Approved for Self-Testing in Australia: Published Diagnostic Test Accuracy Studies and Manufacturer-Supplied Information. A Systematic Review","volume":"219","author":"Bell","year":"2023","journal-title":"Med. J. Aust."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1080\/22221751.2020.1745095","article-title":"Laboratory Diagnosis of Emerging Human Coronavirus Infections\u2014The State of the Art","volume":"9","author":"Loeffelholz","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S0140-6736(21)02346-1","article-title":"Diagnostics for COVID-19: Moving from Pandemic Response to Control","volume":"399","author":"Peeling","year":"2022","journal-title":"Lancet Lond. Engl."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1080\/22221751.2021.1898291","article-title":"Lessons Learned 1 Year after SARS-CoV-2 Emergence Leading to COVID-19 Pandemic","volume":"10","author":"To","year":"2021","journal-title":"Emerg. Microbes Infect."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Y\u00fcce, M., Filiztekin, E., and \u00d6zkaya, K.G. (2021). COVID-19 Diagnosis -A Review of Current Methods. Biosens. Bioelectron., 172.","DOI":"10.1016\/j.bios.2020.112752"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e76","DOI":"10.1056\/NEJMvcm2010260","article-title":"How to Obtain a Nasopharyngeal Swab Specimen","volume":"382","author":"Marty","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_16","unstructured":"(2025, February 26). CDC Interim Guidelines for Collecting and Handling of Clinical Specimens for COVID-19 Testing, Available online: https:\/\/www.cdc.gov\/covid\/hcp\/clinical-care\/clinical-specimen-guidelines.html."},{"key":"ref_17","unstructured":"World Health Organization (2020). Laboratory Testing for Coronavirus Disease 2019 (COVID-19) in Suspected Human Cases: Interim Guidance, 2 March 2020, World Health Organization. Available online: https:\/\/apps.who.int\/iris\/bitstream\/handle\/10665\/331329\/WHO-COVID-19-laboratory-2020.4-eng.pdf."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"495","DOI":"10.34197\/ats-scholar.2020-0109VO","article-title":"Proper Procedures for Performing Nasopharyngeal and Oropharyngeal Swabs for COVID-19","volume":"1","author":"Fazio","year":"2020","journal-title":"ATS Sch."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1177\/0194599820926473","article-title":"The Prevalence of Olfactory and Gustatory Dysfunction in COVID-19 Patients: A Systematic Review and Meta-Analysis","volume":"163","author":"Tong","year":"2020","journal-title":"Otolaryngol. Head Neck Surg. Off. J. Am. Acad. Otolaryngol.-Head Neck Surg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"102777","DOI":"10.1016\/j.amjoto.2020.102777","article-title":"Optimization of COVID-19 Testing Accuracy with Nasal Anatomy Education","volume":"42","author":"Li","year":"2021","journal-title":"Am. J. Otolaryngol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Li, W., Zhou, H., Guo, Q., and Li, G. (2021). The Nasopharynx Swab Test for Coronavirus Disease-2019 Is Mild and Will Not Cause Significant Pain and Anxiety: A Cross-Sectional Study Based on Psychiatrists. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.592092"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.jpsychires.2011.03.006","article-title":"Gender Differences in Anxiety Disorders: Prevalence, Course of Illness, Comorbidity and Burden of Illness","volume":"45","author":"McLean","year":"2011","journal-title":"J. Psychiatr. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"953","DOI":"10.17269\/s41997-020-00434-y","article-title":"Changes in Health Behaviours during Early COVID-19 and Socio-Demographic Disparities: A Cross-Sectional Analysis","volume":"111","author":"Zajacova","year":"2020","journal-title":"Can. J. Public Health"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jhealeco.2009.10.003","article-title":"Understanding Differences in Health Behaviors by Education","volume":"29","author":"Cutler","year":"2010","journal-title":"J. Health Econ."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1097\/j.pain.0000000000000762","article-title":"Sociodemographic Disparities in Chronic Pain, Based on 12-Year Longitudinal Data","volume":"158","year":"2017","journal-title":"Pain"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1093\/geronb\/gbx162","article-title":"Persistent, Consistent, and Extensive: The Trend of Increasing Pain Prevalence in Older Americans","volume":"75","author":"Zimmer","year":"2020","journal-title":"J. Gerontol. B Psychol. Sci. Soc. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e020207","DOI":"10.1136\/bmjopen-2017-020207","article-title":"Education, Job Position, Income or Multidimensional Indices? Associations between Different Socioeconomic Status Indicators and Chronic Low Back Pain in a German Sample: A Longitudinal Field Study","volume":"8","author":"Fliesser","year":"2018","journal-title":"BMJ Open"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1016\/j.jpain.2017.07.005","article-title":"Racial and Socioeconomic Disparities in Disabling Chronic Pain: Findings from the Health and Retirement Study","volume":"18","author":"Janevic","year":"2017","journal-title":"J. Pain"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1097\/j.pain.0000000000000964","article-title":"The Relationship of Sociodemographic and Psychological Variables with Chronic Pain Variables in a Low-Income Population","volume":"158","author":"Newman","year":"2017","journal-title":"Pain"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1093\/cid\/cix461","article-title":"Rapid Tests for Influenza, Respiratory Syncytial Virus, and Other Respiratory Viruses: A Systematic Review and Meta-Analysis","volume":"65","author":"Bruning","year":"2017","journal-title":"Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.jinf.2023.03.005","article-title":"Rapid Multiplex PCR for Respiratory Viruses Reduces Time to Result and Improves Clinical Care: Results of a Systematic Review and Meta-Analysis","volume":"86","author":"Clark","year":"2023","journal-title":"J. Infect."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e2234588","DOI":"10.1001\/jamanetworkopen.2022.34588","article-title":"Diagnostic Accuracy of a Bacterial and Viral Biomarker Point-of-Care Test in the Outpatient Setting","volume":"5","author":"Shapiro","year":"2022","journal-title":"JAMA Netw. Open"},{"key":"ref_33","first-page":"CD015618","article-title":"Laboratory-Based Molecular Test Alternatives to RT-PCR for the Diagnosis of SARS-CoV-2 Infection","volume":"10","author":"Dinnes","year":"2024","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Bashir, I.M., Al-Waleedi, A.A., Al-Shaibani, S.M., Rajamanar, M., Al-Akbari, S., Al-Harazi, A., Salim Aliwah, L., Ahmed Salem, N., Al-Ademi, D., and Barakat, A. (2024). Strengthening Laboratories in Response to Outbreaks in Humanitarian Emergencies and Conflict Settings: Results, Challenges and Lessons from Expanding PCR Diagnostic Capacities for COVID-19 Testing in Yemen. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0298603"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2000763","DOI":"10.1183\/13993003.00763-2020","article-title":"Serology Characteristics of SARS-CoV-2 Infection after Exposure and Post-Symptom Onset","volume":"56","author":"Lou","year":"2020","journal-title":"Eur. Respir. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"11948","DOI":"10.1080\/07391102.2021.1966509","article-title":"Covid-19 Rapid Test by Combining a Random Forest-Based Web System and Blood Tests","volume":"40","author":"Barbosa","year":"2022","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_37","first-page":"CD013705","article-title":"Rapid, Point-of-Care Antigen and Molecular-Based Tests for Diagnosis of SARS-CoV-2 Infection","volume":"3","author":"Dinnes","year":"2021","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.11124\/JBIES-23-00291","article-title":"Comparison of Diagnostic Accuracy of Rapid Antigen Tests for COVID-19 Compared to the Viral Genetic Test in Adults: A Systematic Review and Meta-Analysis","volume":"22","author":"Hirabayashi","year":"2024","journal-title":"JBI Evid. Synth."},{"key":"ref_39","first-page":"e72954","article-title":"Strategies to Overcome Erroneous Outcomes in Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Testing: Insights From the COVID-19 Pandemic","volume":"16","author":"Sajal","year":"2024","journal-title":"Cureus"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e000282","DOI":"10.1136\/bmjph-2023-000282","article-title":"Excess Mortality across Countries in the Western World since the COVID-19 Pandemic: \u2018Our World in Data\u2019 Estimates of January 2020 to December 2022","volume":"2","author":"Mostert","year":"2024","journal-title":"BMJ Public Health"}],"container-title":["Nursing Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2039-4403\/15\/6\/196\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:44:21Z","timestamp":1760031861000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2039-4403\/15\/6\/196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,30]]},"references-count":40,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["nursrep15060196"],"URL":"https:\/\/doi.org\/10.3390\/nursrep15060196","relation":{},"ISSN":["2039-4403"],"issn-type":[{"type":"electronic","value":"2039-4403"}],"subject":[],"published":{"date-parts":[[2025,5,30]]}}}