{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:13:10Z","timestamp":1790010790270,"version":"4.0.1"},"reference-count":38,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nurs. Rep.","Nursing Reports"],"abstract":"<jats:p>Background: The neonatal microbiome plays a fundamental role in immune, metabolic, and physiological development from the earliest stages of life. Its establishment is influenced by several perinatal factors, many of which are closely related to nursing care practices. Objective: This study aimed to identify the principal perinatal factors associated with neonatal microbiome development and, secondarily, to explore their implications for nursing practice based on the available evidence. Methods: An integrative review was conducted using PubMed, Scopus, Web of Science, and CINAHL. Studies published between 2016 and 2026 involving newborns or neonates and addressing factors influencing neonatal microbiome development were considered. Following screening, eligibility assessment, and methodological quality appraisal, 14 studies were included in the final synthesis. Results: The evidence consistently identified mode of delivery, breastfeeding, and perinatal antibiotic exposure as the perinatal factors most consistently associated with neonatal microbial colonization. Vaginal delivery and breastfeeding were associated with greater abundances of beneficial bacterial genera, including Bifidobacterium, Lactobacillus, and Bacteroides, whereas cesarean delivery and antibiotic exposure were associated with reduced microbial diversity and altered colonization patterns. Emerging interventions such as vaginal microbial transfer showed promising results, although current evidence remains limited. Nursing-related practices, particularly breastfeeding support, promotion of early skin-to-skin contact, and participation in antimicrobial stewardship, were identified as key strategies for supporting healthy microbiome development. Conclusions: Current evidence suggests that nursing care may play an important role in promoting healthy neonatal microbial colonization through interventions that support physiological birth processes, breastfeeding, and appropriate antibiotic use. Further research is needed to evaluate the direct impact of specific nursing interventions on neonatal microbiome development and subsequent health outcomes.<\/jats:p>","DOI":"10.3390\/nursrep16090341","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:06:51Z","timestamp":1790006811000},"page":"341","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Perinatal Factors Associated with Neonatal Microbiome Development and Their Implications for Nursing Practice: An Integrative Review"],"prefix":"10.3390","volume":"16","author":[{"given":"Martina","family":"Feliu","sequence":"first","affiliation":[{"name":"Faculty of Nursing and Physiotherapy Salus Infirmorum, Pontifical University of Salamanca, 28015 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8035-2439","authenticated-orcid":false,"given":"Maria","family":"Gallo-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Faculty of Nursing and Physiotherapy Salus Infirmorum, Pontifical University of Salamanca, 28015 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adri\u00e1n","family":"Bouzas","sequence":"additional","affiliation":[{"name":"Faculty of Nursing and Physiotherapy Salus Infirmorum, Pontifical University of Salamanca, 28015 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6990-8944","authenticated-orcid":false,"given":"Maria Isabel","family":"Buceta-Toro","sequence":"additional","affiliation":[{"name":"Faculty of Nursing and Physiotherapy Salus Infirmorum, Pontifical University of Salamanca, 28015 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8483-6766","authenticated-orcid":false,"given":"Clara","family":"Gomez-Urios","sequence":"additional","affiliation":[{"name":"Nutrition and Food Science Area, Faculty of Pharmacy and Food Science, University of Valencia, Av. Vicent Andr\u00e9s Estell\u00e9s 22, 46100 Burjassot, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Microbioma Humano","volume":"76","year":"2016","journal-title":"Rev. Obstet. Ginecol. Venez."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e258","DOI":"10.1542\/neo.20-5-e258","article-title":"The Neonatal Microbiome and Metagenomics: What Do We Know and What Is the Future?","volume":"20","author":"Valentine","year":"2019","journal-title":"Neoreviews"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.cnd.2024.04.006","article-title":"Microbiote Intestinal: De La St\u00e9rilit\u00e9 Chez Les Nouveau-N\u00e9s \u00e0 La Complexit\u00e9 Des Interactions Chez l\u2019adulte","volume":"59","author":"Chakoory","year":"2024","journal-title":"Cah. Nutr. Di\u00e9t\u00e9tique"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"157","DOI":"10.3233\/NHA-210129","article-title":"The Contrasting Human Gut Microbiota in Early and Late Life and Implications for Host Health and Disease","volume":"6","author":"Skillington","year":"2021","journal-title":"Nutr. Healthy Aging"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1038\/s41372-020-00829-2","article-title":"Neonatal Intestinal Dysbiosis","volume":"40","author":"Underwood","year":"2020","journal-title":"J. Perinatol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Al Jehani, A.N., Shuaib, M., Alsharif, A., Alsubaie, K.A., Khraisat, A., Alsharif, A., Altaf, M., Almasry, R.H., Kayali, A.M., and Abdallah, S.A. (2025). Impact of Maternal Microbiota Composition on Neonatal Immunity and Early Childhood Allergies: A Systematic Review. Pediatr. Rep., 17.","DOI":"10.3390\/pediatric17030067"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1186\/s40168-025-02186-8","article-title":"Microbiome in Heritage: How Maternal Microbiome Transmission Impacts next Generation Health","volume":"13","author":"Delaroque","year":"2025","journal-title":"Microbiome"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1097\/NMC.0000000000000373","article-title":"The Maternal Infant Microbiome: Considerations for Labor and Birth","volume":"42","author":"Dunn","year":"2017","journal-title":"MCN Am. J. Matern. Child Nurs."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"a023051","DOI":"10.1101\/cshperspect.a023051","article-title":"The Perinatal Microbiome and Pregnancy: Moving Beyond the Vaginal Microbiome","volume":"5","author":"Prince","year":"2015","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Kozyrskyj, A.L., Bridgman, S.L., and Tun, M.H. (2017). The Impact of Birth and Postnatal Medical Interventions on Infant Gut Microbiota. Microbiota in Health and Disease: From Pregnancy to Childhood, Wageningen Academic Publishers.","DOI":"10.3920\/978-90-8686-839-1_4"},{"key":"ref_11","first-page":"326","article-title":"Early Life Exposures and the Development of the Infant Gut Microbiome: A Review","volume":"89","author":"Li","year":"2021","journal-title":"Pediatr. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Bautista, J., and L\u00f3pez-Cort\u00e9s, A. (2025). Microbial Beginnings: Determinants and Disruptions of the Neonatal Microbiome. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1672780"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Rutayisire, E., Huang, K., Liu, Y., and Tao, F. (2016). The Mode of Delivery Affects the Diversity and Colonization Pattern of the Gut Microbiota during the First Year of Infants\u2019 Life: A Systematic Review. BMC Gastroenterol., 16.","DOI":"10.1186\/s12876-016-0498-0"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Stinson, L.F., Payne, M.S., and Keelan, J.A. (2018). A Critical Review of the Bacterial Baptism Hypothesis and the Impact of Cesarean Delivery on the Infant Microbiome. Front. Med., 5.","DOI":"10.3389\/fmed.2018.00135"},{"key":"ref_15","first-page":"S27","article-title":"Establishment of the Intestinal Microbiota of the Newborn during Breastfeeding","volume":"45","year":"2024","journal-title":"Acta Pediatr. Mex."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Gopalakrishna, K.P., and Hand, T.W. (2020). Influence of Maternal Milk on the Neonatal Intestinal Microbiome. Nutrients, 12.","DOI":"10.3390\/nu12030823"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Cukrowska, B., Bier\u0142a, J.B., Zakrzewska, M., Klukowski, M., and Maciorkowska, E. (2020). The Relationship between the Infant Gut Microbiota and Allergy. The Role of Bifidobacterium breve and Prebiotic Oligosaccharides in the Activation of Anti-Allergic Mechanisms in Early Life. Nutrients, 12.","DOI":"10.3390\/nu12040946"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/s41372-023-01810-5","article-title":"Microbiome Dysbiosis: A Modifiable State and Target to Prevent Staphylococcus Aureus Infections and Other Diseases in Neonates","volume":"44","author":"Aneja","year":"2024","journal-title":"J. Perinatol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Iqbal, F., Shenoy, P.A., Lewis, L.E.S., Siva, N., Purkayastha, J., and Eshwara, V.K. (2025). Influence of Perinatal Antibiotic on Neonatal Gut Microbiota: A Prospective Cohort Study. BMC Pediatr., 25.","DOI":"10.1186\/s12887-025-05907-y"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.siny.2017.07.002","article-title":"Prenatal and Postnatal Administration of Prebiotics and Probiotics","volume":"22","author":"Sohn","year":"2017","journal-title":"Semin. Fetal Neonatal Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1891\/NN-2023-0001","article-title":"Protect the Microbiome: Be HOLISTIC","volume":"42","author":"Wadhwani","year":"2023","journal-title":"Neonatal Netw."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1097\/NMC.0000000000000375","article-title":"The Neonatal Microbiome: Implications for Neonatal Intensive Care Unit Nurses","volume":"42","author":"Rodriguez","year":"2017","journal-title":"MCN Am. J. Matern. Child Nurs."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1159\/000496427","article-title":"Effect of Delivery Mode and Nutrition on Gut Microbiota in Neonates","volume":"74","author":"Akagawa","year":"2019","journal-title":"Ann. Nutr. Metab."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 Statement: An Updated Guideline for Reporting Systematic Reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Oma\u00f1a-Covarrubias, A., Gonz\u00e1lez-Olivares, L.G., L\u00f3pez P\u00f3ntigo, L., Nez-Castro, A.T., Cruz-Mart\u00ednez, R., and Hern\u00e1ndez-Ortega, M. (2025). The Role of Maternal Gut Firmicutes\/Bacteroidetes Ratio in Shaping Fetal Development and Neonatal Microbial Communities. Life, 15.","DOI":"10.3390\/life15070990"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"107458","DOI":"10.1016\/j.ijantimicag.2025.107458","article-title":"Transgenerational Gut Dysbiosis: Unveiling the Dynamics of Antibiotic Resistance through Mobile Genetic Elements from Mothers to Infants","volume":"65","author":"Farooq","year":"2025","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"002000","DOI":"10.1099\/jmm.0.002000","article-title":"Impact of Maternal Obesity and Mode of Delivery on the Newborn Skin and Oral Microbiomes","volume":"74","author":"Seifert","year":"2025","journal-title":"J. Med. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e00491-23","DOI":"10.1128\/mbio.00491-23","article-title":"Maternal Bacterial Engraftment in Multiple Body Sites of Cesarean Section Born Neonates after Vaginal Seeding-a Randomized Controlled Trial","volume":"14","author":"Mueller","year":"2023","journal-title":"mBio"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Matharu, D., Ponsero, A.J., Dikareva, E., Korpela, K., Kolho, K.L., de Vos, W.M., and Salonen, A. (2022). Bacteroides Abundance Drives Birth Mode Dependent Infant Gut Microbiota Developmental Trajectories. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.953475"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Raspini, B., Vacca, M., Porri, D., De Giuseppe, R., Calabrese, F.M., Chieppa, M., Liso, M., Cerbo, R.M., Civardi, E., and Garofoli, F. (2021). Early Life Microbiota Colonization at Six Months of Age: A Transitional Time Point. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.590202"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1007\/s12088-020-00901-7","article-title":"Do Maternal Microbes Shape Newborn Oral Microbes?","volume":"61","author":"Wu","year":"2021","journal-title":"Indian J. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1080\/19490976.2020.1730294","article-title":"Distinct Maternal Microbiota Clusters Are Associated with Diet during Pregnancy: Impact on Neonatal Microbiota and Infant Growth during the First 18 Months of Life","volume":"11","author":"Collado","year":"2020","journal-title":"Gut Microbes"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/mSphere.00984-19","article-title":"Perinatal Antibiotic Exposure Affects the Transmission between Maternal and Neonatal Microbiota and Is Associated with Early-Onset Sepsis","volume":"5","author":"Zhou","year":"2020","journal-title":"mSphere"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"353","DOI":"10.3920\/BM2016.0216","article-title":"Gut Dysbiosis Following C-Section Instigates Higher Colonisation of Toxigenic Clostridium Perfringens in Infants","volume":"8","author":"Nagpal","year":"2017","journal-title":"Benef. Microbes"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/nm.4039","article-title":"Partial Restoration of the Microbiota of Cesarean-Born Infants via Vaginal Microbial Transfer","volume":"22","author":"Shen","year":"2016","journal-title":"Nat. Med."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ajeeb, T.T., Gonzalez, E., Solomons, N.W., Vossenaar, M., and Koski, K.G. (2024). Human Milk Microbiome: Associations with Maternal Diet and Infant Growth. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1341777"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Cabrera-Rubio, R., Pickett-Nairne, K., Gonz\u00e1lez-Solares, S., Collado, M.C., and Venter, C. (2024). The Maternal Diet Index and Offspring Microbiota at 1 Month of Life: Insights from the Mediterranean Birth Cohort MAMI. Nutrients, 16.","DOI":"10.3390\/nu16020314"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Klymiuk, I., Singer, G., Castellani, C., Trajanoski, S., Oberm\u00fcller, B., and Till, H. (2021). Characterization of the Luminal and Mucosa-Associated Microbiome along the Gastrointestinal Tract: Results from Surgically Treated Preterm Infants and a Murine Model. Nutrients, 13.","DOI":"10.3390\/nu13031030"}],"container-title":["Nursing Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2039-4403\/16\/9\/341\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:46:59Z","timestamp":1790009219000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2039-4403\/16\/9\/341"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":38,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["nursrep16090341"],"URL":"https:\/\/doi.org\/10.3390\/nursrep16090341","relation":{},"ISSN":["2039-4403"],"issn-type":[{"value":"2039-4403","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]}}}