{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:55:41Z","timestamp":1780347341064,"version":"3.54.1"},"reference-count":55,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,19]],"date-time":"2021-01-19T00:00:00Z","timestamp":1611014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>During recent years; Xylella fastidiosa subsp. pauca (Xfp) has spread in Salento causing relevant damage to the olive groves. Measures to contain the spreading of the pathogen include the monitoring of the areas bordering the so-called \u201cinfected\u201d zone and the tree eradication in case of positive detection. In order to provide a control strategy aimed to maintain the tree productivity in the infected areas, we further evaluated the in vitro and in planta mid-term effectiveness of a zinc-copper-citric acid biocomplex. The compound showed an in vitro bactericidal activity and inhibited the biofilm formation in representative strains of X. fastidiosa subspecies, including Xfp isolated in Apulia from olive trees. The field mid-term evaluation of the control strategy assessed by quantitative real-time PCR in 41 trees of two olive groves of the \u201cinfected\u201d area revealed a low concentration of Xfp over the seasons upon the regular spraying of the biocomplex over 3 or 4 consecutive years. In particular, the bacterial concentration lowered in July and October with respect to March, after six consecutive treatments. The trend was not affected by the cultivar and it was similar either in the Xfp-sensitive cultivars Ogliarola salentina and Cellina di Nard\u00f2 or in the Xfp-resistant Leccino. Moreover, the scoring of the number of wilted twigs over the seasons confirmed the trend. The efficacy of the treatment in the management of olive groves subjected to a high pathogen pressure is highlighted by the yielded a good oil production<\/jats:p>","DOI":"10.3390\/pathogens10010085","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T03:34:25Z","timestamp":1611113665000},"page":"85","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Further In Vitro Assessment and Mid-Term Evaluation of Control Strategy of Xylella fastidiosa subsp. pauca in Olive Groves of Salento (Apulia, Italy)"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6257-1624","authenticated-orcid":false,"given":"Giuseppe","family":"Tatulli","sequence":"first","affiliation":[{"name":"Council for Agricultural Research and Economics (CREA), Research Centre for Plant Protection and Certification, 00156 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3435-4689","authenticated-orcid":false,"given":"Vanessa","family":"Modesti","sequence":"additional","affiliation":[{"name":"Council for Agricultural Research and Economics (CREA), Research Centre for Plant Protection and Certification, 00156 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7286-4287","authenticated-orcid":false,"given":"Nicoletta","family":"Pucci","sequence":"additional","affiliation":[{"name":"Council for Agricultural Research and Economics (CREA), Research Centre for Plant Protection and Certification, 00156 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9383-8766","authenticated-orcid":false,"given":"Valeria","family":"Scala","sequence":"additional","affiliation":[{"name":"Council for Agricultural Research and Economics (CREA), Research Centre for Plant Protection and Certification, 00156 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6698-6250","authenticated-orcid":false,"given":"Alessia","family":"L\u2019Aurora","sequence":"additional","affiliation":[{"name":"Council for Agricultural Research and Economics (CREA), Research Centre for Plant Protection and Certification, 00156 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7453-4599","authenticated-orcid":false,"given":"Simone","family":"Lucchesi","sequence":"additional","affiliation":[{"name":"Council for Agricultural Research and Economics (CREA), Research Centre for Plant Protection and Certification, 00156 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1088-9520","authenticated-orcid":false,"given":"Manuel","family":"Salustri","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Ambientale, Sapienza University of Rome, 00185 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Scortichini","sequence":"additional","affiliation":[{"name":"Council for Agricultural Research and Economics (CREA), Research Centre for Olive, Fruit and Citrus Crops, 00134 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7761-6186","authenticated-orcid":false,"given":"Stefania","family":"Loreti","sequence":"additional","affiliation":[{"name":"Council for Agricultural Research and Economics (CREA), Research Centre for Plant Protection and Certification, 00156 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,19]]},"reference":[{"key":"ref_1","first-page":"668","article-title":"Identification of DNA sequences related to Xylella fastidiosa in oleander, almond and olive trees exhibiting leaf scorch symptoms in Apulia (southern Italy)","volume":"95","author":"Saponari","year":"2013","journal-title":"J. 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