{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T02:04:24Z","timestamp":1781229864643,"version":"3.54.1"},"reference-count":29,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T00:00:00Z","timestamp":1611100800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U1901208, 31820103014"],"award-info":[{"award-number":["U1901208, 31820103014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013314","name":"Higher Education Discipline Innovation Project","doi-asserted-by":"publisher","award":["D20008"],"award-info":[{"award-number":["D20008"]}],"id":[{"id":"10.13039\/501100013314","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innovation Team Project of Guangdong University","award":["2019KCXTD001"],"award-info":[{"award-number":["2019KCXTD001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.<\/jats:p>","DOI":"10.3390\/pathogens10020089","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T12:16:18Z","timestamp":1611144978000},"page":"89","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China"],"prefix":"10.3390","volume":"10","author":[{"given":"Jiayu","family":"Li","sequence":"first","affiliation":[{"name":"Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuxian","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruobing","family":"Liang","sequence":"additional","affiliation":[{"name":"Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Guo","sequence":"additional","affiliation":[{"name":"Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqiong","family":"Guo","sequence":"additional","affiliation":[{"name":"Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8380-1246","authenticated-orcid":false,"given":"Yaoyu","family":"Feng","sequence":"additional","affiliation":[{"name":"Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Wushan Road, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Xiao","sequence":"additional","affiliation":[{"name":"Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Wushan Road, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Wushan Road, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S1473-3099(14)70772-8","article-title":"A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for Cryptosporidium","volume":"15","author":"Checkley","year":"2015","journal-title":"Lancet Infect. 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