{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T04:22:06Z","timestamp":1784866926668,"version":"3.55.0"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T00:00:00Z","timestamp":1614729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Leptospira borgpetersenii serovar Hardjo (LH) is an important infectious agent of reproduction pathologies and lactation decline in cattle, with a possible zoonotic role. To figure out the potential zoonotic risk for human raw-milk consumption, the present study aims at assessing the persistence and viability of LH in refrigerated raw milk over a 10-day period, which is set as the maximum time range for raw-milk domestic consumption. A negative sample of fresh raw milk was contaminated with an LH strain (2 \u00d7 108 Leptospires\/mL) and analyzed by a rrs (16S) gene targeting real-time PCR (rPCR) protocol for LH DNA at days 1, 2, 3, 6, 7, 9, and 10. Seven aliquots of the same sampling time were inoculated into a semisolid EMJH media for bacterial culture. All aliquots tested positive in both rPCR and culture, which demonstrates that raw milk does not alter the detectability and viability of LH, respectively. The analytical sensitivity (LoD, limit of detection) determined for the rPCR (103 Leptospires\/mL) was repeatable during the study, whereas it gradually decreased when it came to the bacterial culture. This study demonstrates that bovine raw milk might be a potential vehicle of infection by LH, even when storage conditions are strictly respected.<\/jats:p>","DOI":"10.3390\/pathogens10030291","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T20:33:57Z","timestamp":1614803637000},"page":"291","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Persistence of Leptospira borgpetersenii Serovar Hardjo in Refrigerated Raw Milk: A Transmission Risk of Leptospirosis to Humans"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2369-8467","authenticated-orcid":false,"given":"Elisa","family":"Mazzotta","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"},{"name":"Department of Animal Medicine, Productions and Health (MAPS), University of Padova, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Letizia","family":"Ceglie","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Giurisato","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7809-0331","authenticated-orcid":false,"given":"Laura","family":"Bellinati","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-9024","authenticated-orcid":false,"given":"Laura","family":"Lucchese","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Marchione","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2104-4531","authenticated-orcid":false,"given":"Alda","family":"Natale","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1007\/s00044-014-1251-2","article-title":"Structure-based virtual screening and docking studies for the identification of novel inhibitors against wild and drug resistance strains of HIV-1 RT","volume":"24","author":"Chander","year":"2014","journal-title":"Med. Chem. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.tmaid.2009.12.002","article-title":"Leptospirosis: An emerging disease in travellers","volume":"8","author":"Lau","year":"2010","journal-title":"Travel Med. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"702","DOI":"10.3201\/eid0906.020751","article-title":"Leptospirosis in \u201cEco-Challenge\u201d Athletes, Malaysian Borneo","volume":"9","author":"Sejvar","year":"2000","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Costa, F., Hagan, J.E., Calcagno, J., Kane, M., Torgerson, P., Martinez-Silveira, M.S., Stein, C., Abela-Ridder, B., and Ko, A.I. (2015). Global Morbidity and Mortality of Leptospirosis: A Systematic Review. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003898"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.ijid.2007.09.011","article-title":"The globalization of leptospirosis: Worldwide incidence trends","volume":"12","author":"Pappas","year":"2008","journal-title":"Int. J. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1038\/nrmicro2208","article-title":"Leptospira: The dawn of the molecular genetics era for an emerging zoonotic pathogen","volume":"7","author":"Ko","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_8","first-page":"65","article-title":"Leptospirosis in Humans","volume":"387","author":"Haake","year":"2015","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/tbed.12233","article-title":"Leptospirosis in Cattle: A Challenging Scenario for the Understanding of the Epidemiology","volume":"61","author":"Lilenbaum","year":"2014","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1136\/vr.99.20.387","article-title":"Bovine leptospirosis: A serological and clinical study","volume":"99","author":"Ellis","year":"1976","journal-title":"Vet. Rec."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1093\/infdis\/158.1.246","article-title":"Human-to-Human Transmission of Leptospira interrogans by Milk","volume":"158","author":"Bolin","year":"1988","journal-title":"J. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1478-7954-10-5","article-title":"Estimating global mortality from potentially foodborne diseases: An analysis using vital registration data","volume":"10","author":"Hanson","year":"2012","journal-title":"Popul. Health Metr."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Committee on Infectious Diseases, Committee on Nutrition, Brady, M.T., Byington, C.L., Dele Davies, H., Edwards, K.M., Glode, M.P., Jackson, M.A., Keyserling, H.L., and Murray, D.L. (2014). Consumption of Raw or Unpasteurized Milk and Milk Products by Pregnant Women and Children. Pediatrics, 133, 175\u2013179.","DOI":"10.1542\/peds.2013-3502"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"907","DOI":"10.4103\/njcp.njcp_211_17","article-title":"Prevalence of Brucella in raw milk: An example from Turkey","volume":"21","author":"Babaoglu","year":"2018","journal-title":"Niger. J. Clin. Pr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1097\/NT.0000000000000108","article-title":"Raw Milk Consumption","volume":"50","author":"Lucey","year":"2015","journal-title":"Nutr. Today"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1089\/fpd.2005.2.115","article-title":"Foodborne pathogens in milk and the dairy farm environment: Food safety and public health implications","volume":"2","author":"Olivier","year":"2005","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.4315\/0362-028X.JFP-13-213","article-title":"Four-Year Monitoring of Foodborne Pathogens in Raw Milk Sold by Vending Machines in Italy","volume":"76","author":"Giacometti","year":"2013","journal-title":"J. Food Prot."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.vetpar.2005.11.025","article-title":"Application of repeated bulk milk testing for identification of infection dynamics of Neospora caninum in Thai dairy herds","volume":"136","author":"Chanlun","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1093\/biostatistics\/kxp026","article-title":"Bayesian inference for within-herd prevalence of Leptospira interrogans serovar Hardjo using bulk milk antibody testing","volume":"10","author":"Lewis","year":"2009","journal-title":"Biostatistics"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.prevetmed.2012.10.002","article-title":"Temporal trends in bulk milk antibodies to Salmonella, Neospora caninum, and Leptospira interrogans serovar hardjo in Irish dairy herds","volume":"109","author":"Doherty","year":"2013","journal-title":"Prev. Vet. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1017\/S175173111400055X","article-title":"Management practices as risk factors for the presence of bulk milk antibodies to Salmonella, Neospora caninum and Leptospira interrogans serovar hardjo in Irish dairy herds","volume":"8","author":"Berry","year":"2014","journal-title":"Animal"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"9215","DOI":"10.3168\/jds.2016-11863","article-title":"Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain","volume":"100","author":"Velasova","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1186\/2046-0481-57-4-226","article-title":"Prevalence of antibodies to leptospira interrogans serovar hardjo in bulk tank milk from unvaccinated irish dairy herds","volume":"57","author":"Leonard","year":"2004","journal-title":"Ir. Vet. J."},{"key":"ref_24","first-page":"250","article-title":"Leptospirosis in Canada: IV an Atypical Mastitis in Cattle Due to Leptospira Pomona","volume":"23","author":"Mitchell","year":"1959","journal-title":"Can. J. Comp. Med. Vet. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1136\/vr.107.13.307","article-title":"Mastitis and abortion in dairy cattle associated with Leptospira of the serotype hardjo","volume":"107","author":"Higgins","year":"1980","journal-title":"Vet. Rec."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1007\/s12223-016-0452-0","article-title":"Is Leptospira able to survive in raw milk? Study on the inactivation at different storage times and temperatures","volume":"61","author":"Fratini","year":"2016","journal-title":"Folia Microbiol."},{"key":"ref_27","unstructured":"(2021, February 20). Commission Regulation (EU) No.605\/2010 2 July 2010. Official Journal of the European Union 1\u201324. Available online: https:\/\/eur-lex.europa.eu\/oj\/direct-access.html."},{"key":"ref_28","first-page":"560","article-title":"Antileptospiral Effect of Milk","volume":"54","author":"Kirschner","year":"1955","journal-title":"N. Z. Med. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/jb.89.2.545-545.1965","article-title":"Leptospiral Lysis by Lipids of Renal Tissue and Milk","volume":"89","author":"Stalheim","year":"1965","journal-title":"J. Bacteriol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.micpath.2017.03.032","article-title":"Bovine leptospirosis: Prevalence, associated risk factors for infection and their cause-effect relation","volume":"107","author":"Lilenbaum","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_31","unstructured":"Scatozza, R., and Farina, F. (2006). Spirochete. Trattato di Malattie Infettive Degli Animali, UTET."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1017\/S0950268800048196","article-title":"Zoonotic infections in Northern Ireland farmers","volume":"105","author":"Stanford","year":"1990","journal-title":"Epidemiol. Infect."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","article-title":"Leptospirosis: A zoonotic disease of global importance","volume":"3","author":"Bharti","year":"2003","journal-title":"Lancet Infect. Dis."},{"key":"ref_34","first-page":"24","article-title":"Risk of transmission of leptospirosis from infected cattle to dairy workers in southern Israel","volume":"6","author":"Belmaker","year":"2004","journal-title":"Isr. Med. Assoc. J. IMAJ"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1136\/bmj.1.5638.228","article-title":"Leptospirosis in Human Pregnancy followed by Death of the Foetus","volume":"1","author":"Coghlan","year":"1969","journal-title":"BMJ"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Haake, D.A., and Yang, X.F. (2019). Spirochetes. Encyclopedia of Microbiology, Elsevier.","DOI":"10.1016\/B978-0-12-801238-3.02303-5"},{"key":"ref_37","unstructured":"OIE (Office International des Epizooties) (2018). LEPTOSPIROSIS. Man. Diagn. Tests Vaccines Terr. Anim., 2018, 503\u2013516."},{"key":"ref_38","unstructured":"(Official Journal of the European Union, 2017). Commission Regulation (EU) No. 2017\/625, Official Journal of the European Union."},{"key":"ref_39","unstructured":"(Official Journal of the European Union, 2016). Commission Regulation (EU) No. 2016\/429, Official Journal of the European Union."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.1128\/jb.88.6.1618-1623.1964","article-title":"Differentiation of pathogenic and saprophytic leptospires with 8-Azaguanine","volume":"88","author":"Johnson","year":"1964","journal-title":"J. Bacteriol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1128\/jb.76.6.669-670.1958","article-title":"Simple method for separating leptospirae from contaminating microorganisms","volume":"76","author":"Rittenberg","year":"1958","journal-title":"J. Bacteriol."},{"key":"ref_42","first-page":"11","article-title":"Systematics of Leptospiraceae","volume":"387","author":"Levett","year":"2014","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1136\/bmj.1.5693.433-b","article-title":"Leptospirosis","volume":"1","author":"Turner","year":"1970","journal-title":"Br. Med. J."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Smythe, L.D., Smith, I.L., Smith, G.A., Dohnt, M.F., Symonds, M.L., Barnett, L.J., and McKay, D.B. (2002). A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp.. BMC Infect. Dis., 2.","DOI":"10.1186\/1471-2334-2-13"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/3\/291\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:32:14Z","timestamp":1760160734000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/3\/291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,3]]},"references-count":44,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["pathogens10030291"],"URL":"https:\/\/doi.org\/10.3390\/pathogens10030291","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,3]]}}}