{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:38:02Z","timestamp":1780346282474,"version":"3.54.1"},"reference-count":34,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,13]],"date-time":"2021-03-13T00:00:00Z","timestamp":1615593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Intraocular inflammation following mycobacterial dissemination to the eye is common in tuberculosis (TB)-endemic countries. However, the early host\u2013pathogen interactions during ocular dissemination are unknown. In this study, we investigated the early events during mycobacterial invasion of the blood-retinal barriers (BRBs) with fluorescent-tagged Mycobacterium marinum (Mm), host macrophages, and retinal vasculature in a zebrafish model of ocular TB. We found that Mm invaded the vascular endothelium in either the extracellular or intracellular (inside phagocytes) state, typically 3\u20134 days post-injection (dpi). Extracellular Mm are phagocytosed in the retinal tissue and progress to form a compact granuloma around 6 dpi. Intracellular Mm crossing the BRBs are likely to be less virulent and either persist inside solitary macrophages (in most cases) or progress to loosely arranged granuloma (rarely). The early interactions between mycobacteria and host immune cells can thus determine the course of disease during mycobacterial dissemination to the eye.<\/jats:p>","DOI":"10.3390\/pathogens10030333","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T23:52:06Z","timestamp":1615765926000},"page":"333","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Role of Extracellular Mycobacteria in Blood-Retinal Barrier Invasion in a Zebrafish Model of Ocular TB"],"prefix":"10.3390","volume":"10","author":[{"given":"Santhosh Kumar","family":"Damera","sequence":"first","affiliation":[{"name":"Allhadini Mishra Zebrafish Facility, Brien Holden Eye Research Centre, L V Prasad Eye Institute (MTC Campus), Bhubaneswar 751024, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjan Kumar","family":"Panigrahi","sequence":"additional","affiliation":[{"name":"Allhadini Mishra Zebrafish Facility, Brien Holden Eye Research Centre, L V Prasad Eye Institute (MTC Campus), Bhubaneswar 751024, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2773-3568","authenticated-orcid":false,"given":"Sanchita","family":"Mitra","sequence":"additional","affiliation":[{"name":"Ocular Microbiology Service, L V Prasad Eye Institute (MTC Campus), Bhubaneswar 751024, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soumyava","family":"Basu","sequence":"additional","affiliation":[{"name":"Allhadini Mishra Zebrafish Facility, Brien Holden Eye Research Centre, L V Prasad Eye Institute (MTC Campus), Bhubaneswar 751024, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S46","DOI":"10.1080\/09273948.2016.1255761","article-title":"Epidemiology of Uveitis in a Tertiary-care Referral Institute in North India","volume":"25","author":"Dogra","year":"2017","journal-title":"Ocul. 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