{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T17:57:09Z","timestamp":1781373429880,"version":"3.54.1"},"reference-count":61,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,13]],"date-time":"2021-03-13T00:00:00Z","timestamp":1615593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Cryptosporidiosis is one of the most important causes of gastroenteritis in the world, especially in low- and middle-income countries. It is caused by the Apicomplexan parasite Cryptosporidium spp., and mainly affects children and immunocompromised people, in whom it can pose a serious threat to their health, or even be life threatening. In Honduras, there are no data on parasite species or on molecular diversity or Cryptosporidium subtypes. Therefore, a cross-sectional study was conducted between September 2019 and March 2020 for the molecular identification of Cryptosporidium spp. in 102 patients living with HIV who attended a national hospital in Tegucigalpa. Stool samples were analyzed by direct microscopy, acid-fast stained smears, and a rapid lateral flow immunochromatographic test. All samples that tested positive were molecularly analyzed to identify the species and subtype of the parasite using three different markers: gp60, cowp, and 18Sr. PCR products were also sequenced. Four out of 102 samples (3.92%) were positive for Cryptosporidiumparvum, and all were assigned to subtype IIa. These findings suggest a possible zoonotic transmission in this population.<\/jats:p>","DOI":"10.3390\/pathogens10030336","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T23:52:06Z","timestamp":1615765926000},"page":"336","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras"],"prefix":"10.3390","volume":"10","author":[{"given":"Sergio","family":"Betancourth","sequence":"first","affiliation":[{"name":"Microbiology Research Institute, Universidad Nacional Aut\u00f3noma de Honduras, Tegucigalpa 11101, Honduras"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osman","family":"Archaga","sequence":"additional","affiliation":[{"name":"Microbiology Research Institute, Universidad Nacional Aut\u00f3noma de Honduras, Tegucigalpa 11101, Honduras"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy","family":"Moncada","sequence":"additional","affiliation":[{"name":"Servicio de Atenci\u00f3n Integral para Pacientes que Viven con VIH\/SIDA-Instituto Nacional Cardiopulmonar, Tegucigalpa 11101, Honduras"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vilma","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Servicio de Atenci\u00f3n Integral para Pacientes que Viven con VIH\/SIDA-Instituto Nacional Cardiopulmonar, Tegucigalpa 11101, Honduras"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9756-4520","authenticated-orcid":false,"given":"Gustavo","family":"Fontecha","sequence":"additional","affiliation":[{"name":"Microbiology Research Institute, Universidad Nacional Aut\u00f3noma de Honduras, Tegucigalpa 11101, Honduras"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,13]]},"reference":[{"key":"ref_1","unstructured":"UNAIDS (2021, January 24). 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